Cargando…
ISOLATION AND CHARACTERIZATION OF CHEMICAL CONSTITUENTS FROM CHRYSOPHYLLUM ALBIDUM G. DON-HOLL. STEM-BARK EXTRACTS AND THEIR ANTIOXIDANT AND ANTIBACTERIAL PROPERTIES
BACKGROUND: The plant, Chrysophyllum albidum is indigenous to Nigeria and its stem-bark has wide application in traditional medicine for the treatment of infections and oxidative stress related diseases. The aim of the study was to isolate the chemical constituents responsible for the antioxidant an...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
African Traditional Herbal Medicine Supporters Initiative (ATHMSI)
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5416639/ https://www.ncbi.nlm.nih.gov/pubmed/28487910 http://dx.doi.org/10.21010/ajtcam.v13i5.24 |
_version_ | 1783233786311344128 |
---|---|
author | Idowu, T.O. Ogundaini, A. O. Adesanya, S. A. Onawunmi, G.O. Osungunna, M. O. Obuotor, E. M. Abegaz, B. M. |
author_facet | Idowu, T.O. Ogundaini, A. O. Adesanya, S. A. Onawunmi, G.O. Osungunna, M. O. Obuotor, E. M. Abegaz, B. M. |
author_sort | Idowu, T.O. |
collection | PubMed |
description | BACKGROUND: The plant, Chrysophyllum albidum is indigenous to Nigeria and its stem-bark has wide application in traditional medicine for the treatment of infections and oxidative stress related diseases. The aim of the study was to isolate the chemical constituents responsible for the antioxidant and antibacterial activity from the stem-bark of the plant in order to justify some of its ethnomedicinal uses. MATERIALS AND METHODS: Crude extract of stem-bark of Chrysophyllum albidum obtained from 80% ethanol was successively partitioned with ethyl acetate (EtOAc) and n-butanol. The solvent fractions and isolated compounds were tested for antioxidant property using 2-2-diphenyl-1-picrylhydrazyl. Antibacterial activities were also assessed by means of agar-diffusion and broth micro dilution methods. EtOAc fraction was repeatedly fractionated on column chromatography to afford four compounds and their chemical structures were established using NMR (1D and 2D) and MS. RESULTS: Chromatographic fractionation of EtOAc fraction with the highest antioxidant and antimicrobial activities afforded stigmasterol (1),: epicatechin (2),: epigallocatechin (3): and procyanidin B5 (4).: Procyanidin B5 isolated for the first time from genus Chrysophyllum demonstrated the highest antioxidant activity with IC50 values of 8.8 μM and 11.20 μM in DPPH and nitric oxide assays respectively and equally demonstrated the highest inhibitory activity against Escherichia coli (MIC 156.25 μg/mL), Staphylococcus aureus (MIC 156.25 μg/mL), Pseudomonas aeruginosa (MIC 625 μg/mL) and Bacillus subtilis (MIC 156.25 μg/mL). CONCLUSION: The antibacterial and antioxidant activities of epicatechin, epigallocatechin and procyanidin B5 isolated from Chrysophyllum albidum stem-bark validate the folkloric uses. |
format | Online Article Text |
id | pubmed-5416639 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | African Traditional Herbal Medicine Supporters Initiative (ATHMSI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-54166392017-08-12 ISOLATION AND CHARACTERIZATION OF CHEMICAL CONSTITUENTS FROM CHRYSOPHYLLUM ALBIDUM G. DON-HOLL. STEM-BARK EXTRACTS AND THEIR ANTIOXIDANT AND ANTIBACTERIAL PROPERTIES Idowu, T.O. Ogundaini, A. O. Adesanya, S. A. Onawunmi, G.O. Osungunna, M. O. Obuotor, E. M. Abegaz, B. M. Afr J Tradit Complement Altern Med Article BACKGROUND: The plant, Chrysophyllum albidum is indigenous to Nigeria and its stem-bark has wide application in traditional medicine for the treatment of infections and oxidative stress related diseases. The aim of the study was to isolate the chemical constituents responsible for the antioxidant and antibacterial activity from the stem-bark of the plant in order to justify some of its ethnomedicinal uses. MATERIALS AND METHODS: Crude extract of stem-bark of Chrysophyllum albidum obtained from 80% ethanol was successively partitioned with ethyl acetate (EtOAc) and n-butanol. The solvent fractions and isolated compounds were tested for antioxidant property using 2-2-diphenyl-1-picrylhydrazyl. Antibacterial activities were also assessed by means of agar-diffusion and broth micro dilution methods. EtOAc fraction was repeatedly fractionated on column chromatography to afford four compounds and their chemical structures were established using NMR (1D and 2D) and MS. RESULTS: Chromatographic fractionation of EtOAc fraction with the highest antioxidant and antimicrobial activities afforded stigmasterol (1),: epicatechin (2),: epigallocatechin (3): and procyanidin B5 (4).: Procyanidin B5 isolated for the first time from genus Chrysophyllum demonstrated the highest antioxidant activity with IC50 values of 8.8 μM and 11.20 μM in DPPH and nitric oxide assays respectively and equally demonstrated the highest inhibitory activity against Escherichia coli (MIC 156.25 μg/mL), Staphylococcus aureus (MIC 156.25 μg/mL), Pseudomonas aeruginosa (MIC 625 μg/mL) and Bacillus subtilis (MIC 156.25 μg/mL). CONCLUSION: The antibacterial and antioxidant activities of epicatechin, epigallocatechin and procyanidin B5 isolated from Chrysophyllum albidum stem-bark validate the folkloric uses. African Traditional Herbal Medicine Supporters Initiative (ATHMSI) 2016-08-12 /pmc/articles/PMC5416639/ /pubmed/28487910 http://dx.doi.org/10.21010/ajtcam.v13i5.24 Text en Copyright: © 2016 Afr. J. Traditional Complementary and Alternative Medicines http://creativecommons.org/licenses/CC-BY/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License |
spellingShingle | Article Idowu, T.O. Ogundaini, A. O. Adesanya, S. A. Onawunmi, G.O. Osungunna, M. O. Obuotor, E. M. Abegaz, B. M. ISOLATION AND CHARACTERIZATION OF CHEMICAL CONSTITUENTS FROM CHRYSOPHYLLUM ALBIDUM G. DON-HOLL. STEM-BARK EXTRACTS AND THEIR ANTIOXIDANT AND ANTIBACTERIAL PROPERTIES |
title | ISOLATION AND CHARACTERIZATION OF CHEMICAL CONSTITUENTS FROM CHRYSOPHYLLUM ALBIDUM G. DON-HOLL. STEM-BARK EXTRACTS AND THEIR ANTIOXIDANT AND ANTIBACTERIAL PROPERTIES |
title_full | ISOLATION AND CHARACTERIZATION OF CHEMICAL CONSTITUENTS FROM CHRYSOPHYLLUM ALBIDUM G. DON-HOLL. STEM-BARK EXTRACTS AND THEIR ANTIOXIDANT AND ANTIBACTERIAL PROPERTIES |
title_fullStr | ISOLATION AND CHARACTERIZATION OF CHEMICAL CONSTITUENTS FROM CHRYSOPHYLLUM ALBIDUM G. DON-HOLL. STEM-BARK EXTRACTS AND THEIR ANTIOXIDANT AND ANTIBACTERIAL PROPERTIES |
title_full_unstemmed | ISOLATION AND CHARACTERIZATION OF CHEMICAL CONSTITUENTS FROM CHRYSOPHYLLUM ALBIDUM G. DON-HOLL. STEM-BARK EXTRACTS AND THEIR ANTIOXIDANT AND ANTIBACTERIAL PROPERTIES |
title_short | ISOLATION AND CHARACTERIZATION OF CHEMICAL CONSTITUENTS FROM CHRYSOPHYLLUM ALBIDUM G. DON-HOLL. STEM-BARK EXTRACTS AND THEIR ANTIOXIDANT AND ANTIBACTERIAL PROPERTIES |
title_sort | isolation and characterization of chemical constituents from chrysophyllum albidum g. don-holl. stem-bark extracts and their antioxidant and antibacterial properties |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5416639/ https://www.ncbi.nlm.nih.gov/pubmed/28487910 http://dx.doi.org/10.21010/ajtcam.v13i5.24 |
work_keys_str_mv | AT idowuto isolationandcharacterizationofchemicalconstituentsfromchrysophyllumalbidumgdonhollstembarkextractsandtheirantioxidantandantibacterialproperties AT ogundainiao isolationandcharacterizationofchemicalconstituentsfromchrysophyllumalbidumgdonhollstembarkextractsandtheirantioxidantandantibacterialproperties AT adesanyasa isolationandcharacterizationofchemicalconstituentsfromchrysophyllumalbidumgdonhollstembarkextractsandtheirantioxidantandantibacterialproperties AT onawunmigo isolationandcharacterizationofchemicalconstituentsfromchrysophyllumalbidumgdonhollstembarkextractsandtheirantioxidantandantibacterialproperties AT osungunnamo isolationandcharacterizationofchemicalconstituentsfromchrysophyllumalbidumgdonhollstembarkextractsandtheirantioxidantandantibacterialproperties AT obuotorem isolationandcharacterizationofchemicalconstituentsfromchrysophyllumalbidumgdonhollstembarkextractsandtheirantioxidantandantibacterialproperties AT abegazbm isolationandcharacterizationofchemicalconstituentsfromchrysophyllumalbidumgdonhollstembarkextractsandtheirantioxidantandantibacterialproperties |