Cargando…

Identification of Four ATP-Binding Cassette Transporter Genes in Cnaphalocrocis medinalis and Their Expression in Response to Insecticide Treatment

The ATP-binding cassette (ABC) transporters belong to a superfamily of genes involved in the transport of specific molecules across lipid membranes, as well as insecticide resistance, present in all living organisms. In this study, we combined the Cnaphalocrocis medinals transcriptome database with...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Hai-Zhong, Xu, Jia-Ping, Wang, Xue-Yang, Ma, Yan, Yu, Dong, Fei, Dong-Qiong, Zhang, Shang-Zhi, Wang, Wan-Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5416764/
https://www.ncbi.nlm.nih.gov/pubmed/28365766
http://dx.doi.org/10.1093/jisesa/iex017
_version_ 1783233815602266112
author Yu, Hai-Zhong
Xu, Jia-Ping
Wang, Xue-Yang
Ma, Yan
Yu, Dong
Fei, Dong-Qiong
Zhang, Shang-Zhi
Wang, Wan-Ling
author_facet Yu, Hai-Zhong
Xu, Jia-Ping
Wang, Xue-Yang
Ma, Yan
Yu, Dong
Fei, Dong-Qiong
Zhang, Shang-Zhi
Wang, Wan-Ling
author_sort Yu, Hai-Zhong
collection PubMed
description The ATP-binding cassette (ABC) transporters belong to a superfamily of genes involved in the transport of specific molecules across lipid membranes, as well as insecticide resistance, present in all living organisms. In this study, we combined the Cnaphalocrocis medinals transcriptome database with a bioinformatics approach to identify four C. medinals ABCs (CmABCs), including CmABCG1, CmABCG4, CmABCC2 and CmABCC3. Tissue expression analysis showed that these genes had a tissue-specific expression pattern. CmABCG1 had significantly higher expression in the haemolymph and head compared to the other tissues. The expression of CmABCG4, CmABCC2 and CmABCC3 was highest in the midgut, followed by expression in the fat body. The developmental stage expression analysis showed that CmABCG1, CmABCG4, CmABCC2 and CmABCC3 were mainly expressed in adults. The transcription of CmABCG1, CmABCG4 and CmABCC2 was significantly induced by chlorpyrifos. Taken together, the results of our study provided useful information for understanding of the detoxification system of C. medinalis.
format Online
Article
Text
id pubmed-5416764
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-54167642017-05-05 Identification of Four ATP-Binding Cassette Transporter Genes in Cnaphalocrocis medinalis and Their Expression in Response to Insecticide Treatment Yu, Hai-Zhong Xu, Jia-Ping Wang, Xue-Yang Ma, Yan Yu, Dong Fei, Dong-Qiong Zhang, Shang-Zhi Wang, Wan-Ling J Insect Sci Research Article The ATP-binding cassette (ABC) transporters belong to a superfamily of genes involved in the transport of specific molecules across lipid membranes, as well as insecticide resistance, present in all living organisms. In this study, we combined the Cnaphalocrocis medinals transcriptome database with a bioinformatics approach to identify four C. medinals ABCs (CmABCs), including CmABCG1, CmABCG4, CmABCC2 and CmABCC3. Tissue expression analysis showed that these genes had a tissue-specific expression pattern. CmABCG1 had significantly higher expression in the haemolymph and head compared to the other tissues. The expression of CmABCG4, CmABCC2 and CmABCC3 was highest in the midgut, followed by expression in the fat body. The developmental stage expression analysis showed that CmABCG1, CmABCG4, CmABCC2 and CmABCC3 were mainly expressed in adults. The transcription of CmABCG1, CmABCG4 and CmABCC2 was significantly induced by chlorpyrifos. Taken together, the results of our study provided useful information for understanding of the detoxification system of C. medinalis. Oxford University Press 2017-03-20 /pmc/articles/PMC5416764/ /pubmed/28365766 http://dx.doi.org/10.1093/jisesa/iex017 Text en © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Article
Yu, Hai-Zhong
Xu, Jia-Ping
Wang, Xue-Yang
Ma, Yan
Yu, Dong
Fei, Dong-Qiong
Zhang, Shang-Zhi
Wang, Wan-Ling
Identification of Four ATP-Binding Cassette Transporter Genes in Cnaphalocrocis medinalis and Their Expression in Response to Insecticide Treatment
title Identification of Four ATP-Binding Cassette Transporter Genes in Cnaphalocrocis medinalis and Their Expression in Response to Insecticide Treatment
title_full Identification of Four ATP-Binding Cassette Transporter Genes in Cnaphalocrocis medinalis and Their Expression in Response to Insecticide Treatment
title_fullStr Identification of Four ATP-Binding Cassette Transporter Genes in Cnaphalocrocis medinalis and Their Expression in Response to Insecticide Treatment
title_full_unstemmed Identification of Four ATP-Binding Cassette Transporter Genes in Cnaphalocrocis medinalis and Their Expression in Response to Insecticide Treatment
title_short Identification of Four ATP-Binding Cassette Transporter Genes in Cnaphalocrocis medinalis and Their Expression in Response to Insecticide Treatment
title_sort identification of four atp-binding cassette transporter genes in cnaphalocrocis medinalis and their expression in response to insecticide treatment
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5416764/
https://www.ncbi.nlm.nih.gov/pubmed/28365766
http://dx.doi.org/10.1093/jisesa/iex017
work_keys_str_mv AT yuhaizhong identificationoffouratpbindingcassettetransportergenesincnaphalocrocismedinalisandtheirexpressioninresponsetoinsecticidetreatment
AT xujiaping identificationoffouratpbindingcassettetransportergenesincnaphalocrocismedinalisandtheirexpressioninresponsetoinsecticidetreatment
AT wangxueyang identificationoffouratpbindingcassettetransportergenesincnaphalocrocismedinalisandtheirexpressioninresponsetoinsecticidetreatment
AT mayan identificationoffouratpbindingcassettetransportergenesincnaphalocrocismedinalisandtheirexpressioninresponsetoinsecticidetreatment
AT yudong identificationoffouratpbindingcassettetransportergenesincnaphalocrocismedinalisandtheirexpressioninresponsetoinsecticidetreatment
AT feidongqiong identificationoffouratpbindingcassettetransportergenesincnaphalocrocismedinalisandtheirexpressioninresponsetoinsecticidetreatment
AT zhangshangzhi identificationoffouratpbindingcassettetransportergenesincnaphalocrocismedinalisandtheirexpressioninresponsetoinsecticidetreatment
AT wangwanling identificationoffouratpbindingcassettetransportergenesincnaphalocrocismedinalisandtheirexpressioninresponsetoinsecticidetreatment