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First report and molecular identification of Opisthorchis viverrini infection in human communities from Lower Myanmar

Opisthorchis viverrini is endemic in the South East Asian region, especially in Cambodia, Lao People's Democratic Republic, Vietnam and Thailand, but there have been no previous records from Myanmar. During stool surveys of rural populations in three regions of Lower Myanmar, Opisthorchis-like...

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Autores principales: Aung, Win Pa Pa, Htoon, Thi Thi, Tin, Htay Htay, Thinn, Kyi Kyi, Sanpool, Oranuch, Jongthawin, Jurairat, Sadaow, Lakkhana, Phosuk, Issarapong, Rodpai, Rutchanee, Intapan, Pewpan M., Maleewong, Wanchai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5417708/
https://www.ncbi.nlm.nih.gov/pubmed/28472153
http://dx.doi.org/10.1371/journal.pone.0177130
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author Aung, Win Pa Pa
Htoon, Thi Thi
Tin, Htay Htay
Thinn, Kyi Kyi
Sanpool, Oranuch
Jongthawin, Jurairat
Sadaow, Lakkhana
Phosuk, Issarapong
Rodpai, Rutchanee
Intapan, Pewpan M.
Maleewong, Wanchai
author_facet Aung, Win Pa Pa
Htoon, Thi Thi
Tin, Htay Htay
Thinn, Kyi Kyi
Sanpool, Oranuch
Jongthawin, Jurairat
Sadaow, Lakkhana
Phosuk, Issarapong
Rodpai, Rutchanee
Intapan, Pewpan M.
Maleewong, Wanchai
author_sort Aung, Win Pa Pa
collection PubMed
description Opisthorchis viverrini is endemic in the South East Asian region, especially in Cambodia, Lao People's Democratic Republic, Vietnam and Thailand, but there have been no previous records from Myanmar. During stool surveys of rural populations in three regions of Lower Myanmar, Opisthorchis-like eggs were found in 34 out of 364 (9.3%) participants by stool microscopy after using the modified formalin-ether concentration technique. DNA was extracted from these positive stool samples and a portion of the mitochondrial cytochrome c oxidase subunit I (cox1) gene was amplified using the polymerase chain reaction and then sequenced. DNA sequences, successfully obtained from 18 of 34 positive samples (Bago Region, n = 13; Mon State, n = 3; Yangon Region, n = 2), confirmed that the eggs were of O. viverrini. Sequences showed 99.7% identity with O. viverrini mitochondrial cox1 (GenBank accession no. JF739555) but 95%, 88.7%, 82.6% and 81.4% identities with those of Opisthorchis lobatus from Lao People's Democratic Republic (GenBank accession nos. HQ328539-HQ328541), Metorchis orientalis from China (KT239342), Clonorchis sinensis from China (JF729303) and Opisthorchis felineus from Russia (EU921260), respectively. When alignement with other Opisthorchiidae trematodes, 81% similarity with Metorchis bilis from Czech Republic (GenBank accession nos. KT740966, KT740969, KT740970) and Slovakia (GenBank accession nos. KT740971-KT740973), 84.6% similarity with Metorchis xanthosomus from Czech Republic (GenBank accession no. KT740974), 78.6% similarity with M. xanthosomus from Poland (GenBank accession no. KT740968) and 82.2% similarity with Euamphimerus pancreaticus from Czech Republic (GenBank accession no. KT740975) were revealed. This study demonstrated, for the first time, O. viverrini from rural people in Myanmar using molecular methods and is an urgent call for surveillance and control activities against opisthorchiasis in Myanmar.
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spelling pubmed-54177082017-05-14 First report and molecular identification of Opisthorchis viverrini infection in human communities from Lower Myanmar Aung, Win Pa Pa Htoon, Thi Thi Tin, Htay Htay Thinn, Kyi Kyi Sanpool, Oranuch Jongthawin, Jurairat Sadaow, Lakkhana Phosuk, Issarapong Rodpai, Rutchanee Intapan, Pewpan M. Maleewong, Wanchai PLoS One Research Article Opisthorchis viverrini is endemic in the South East Asian region, especially in Cambodia, Lao People's Democratic Republic, Vietnam and Thailand, but there have been no previous records from Myanmar. During stool surveys of rural populations in three regions of Lower Myanmar, Opisthorchis-like eggs were found in 34 out of 364 (9.3%) participants by stool microscopy after using the modified formalin-ether concentration technique. DNA was extracted from these positive stool samples and a portion of the mitochondrial cytochrome c oxidase subunit I (cox1) gene was amplified using the polymerase chain reaction and then sequenced. DNA sequences, successfully obtained from 18 of 34 positive samples (Bago Region, n = 13; Mon State, n = 3; Yangon Region, n = 2), confirmed that the eggs were of O. viverrini. Sequences showed 99.7% identity with O. viverrini mitochondrial cox1 (GenBank accession no. JF739555) but 95%, 88.7%, 82.6% and 81.4% identities with those of Opisthorchis lobatus from Lao People's Democratic Republic (GenBank accession nos. HQ328539-HQ328541), Metorchis orientalis from China (KT239342), Clonorchis sinensis from China (JF729303) and Opisthorchis felineus from Russia (EU921260), respectively. When alignement with other Opisthorchiidae trematodes, 81% similarity with Metorchis bilis from Czech Republic (GenBank accession nos. KT740966, KT740969, KT740970) and Slovakia (GenBank accession nos. KT740971-KT740973), 84.6% similarity with Metorchis xanthosomus from Czech Republic (GenBank accession no. KT740974), 78.6% similarity with M. xanthosomus from Poland (GenBank accession no. KT740968) and 82.2% similarity with Euamphimerus pancreaticus from Czech Republic (GenBank accession no. KT740975) were revealed. This study demonstrated, for the first time, O. viverrini from rural people in Myanmar using molecular methods and is an urgent call for surveillance and control activities against opisthorchiasis in Myanmar. Public Library of Science 2017-05-04 /pmc/articles/PMC5417708/ /pubmed/28472153 http://dx.doi.org/10.1371/journal.pone.0177130 Text en © 2017 Aung et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Aung, Win Pa Pa
Htoon, Thi Thi
Tin, Htay Htay
Thinn, Kyi Kyi
Sanpool, Oranuch
Jongthawin, Jurairat
Sadaow, Lakkhana
Phosuk, Issarapong
Rodpai, Rutchanee
Intapan, Pewpan M.
Maleewong, Wanchai
First report and molecular identification of Opisthorchis viverrini infection in human communities from Lower Myanmar
title First report and molecular identification of Opisthorchis viverrini infection in human communities from Lower Myanmar
title_full First report and molecular identification of Opisthorchis viverrini infection in human communities from Lower Myanmar
title_fullStr First report and molecular identification of Opisthorchis viverrini infection in human communities from Lower Myanmar
title_full_unstemmed First report and molecular identification of Opisthorchis viverrini infection in human communities from Lower Myanmar
title_short First report and molecular identification of Opisthorchis viverrini infection in human communities from Lower Myanmar
title_sort first report and molecular identification of opisthorchis viverrini infection in human communities from lower myanmar
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5417708/
https://www.ncbi.nlm.nih.gov/pubmed/28472153
http://dx.doi.org/10.1371/journal.pone.0177130
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