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Complete Freund's adjuvant induces experimental autoimmune myocarditis by enhancing IL‐6 production during initiation of the immune response

INTRODUCTION: Complete Freund's Adjuvant (CFA) emulsified with an antigen is a widely used method to induce autoimmune disease in animal models, yet the contribution of CFA to the immune response is not well understood. We compared the effectiveness of CFA with Incomplete Freund's Adjuvant...

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Autores principales: Fontes, Jillian A., Barin, Jobert G., Talor, Monica V., Stickel, Natalie, Schaub, Julie, Rose, Noel R., Čiháková, Daniela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5418134/
https://www.ncbi.nlm.nih.gov/pubmed/28474508
http://dx.doi.org/10.1002/iid3.155
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author Fontes, Jillian A.
Barin, Jobert G.
Talor, Monica V.
Stickel, Natalie
Schaub, Julie
Rose, Noel R.
Čiháková, Daniela
author_facet Fontes, Jillian A.
Barin, Jobert G.
Talor, Monica V.
Stickel, Natalie
Schaub, Julie
Rose, Noel R.
Čiháková, Daniela
author_sort Fontes, Jillian A.
collection PubMed
description INTRODUCTION: Complete Freund's Adjuvant (CFA) emulsified with an antigen is a widely used method to induce autoimmune disease in animal models, yet the contribution of CFA to the immune response is not well understood. We compared the effectiveness of CFA with Incomplete Freund's Adjuvant (IFA) or TiterMax Gold Adjuvant (TMax) in experimental autoimmune myocarditis (EAM) in male mice. METHODS: EAM was induced in A/J, BALB/c, and IL6KO BALB/c male mice by injection of the myocarditogenic peptide in CFA, IFA, or TMax on days 0 and 7. EAM severity was analyzed by histology on day 21. In addition, specific flow cytometry outcomes were evaluated on day 21. RESULTS: Only mice immunized with CFA and myocarditogenic peptide on both days 0 and 7 developed substantial myocarditis as measured by histology. We observed a significantly increased level of IL6 in the spleen 3 days after CFA immunization. In the spleen and heart on day 21, there was an expansion of myeloid cells in CFA‐immunized mice, as compared to IFA or TMax‐immunized animals. Recombinant IL‐6 at the time of IFA immunization partially restored susceptibility of the mice to EAM. We also treated EAM‐resistant IL‐6 knockout mice with recombinant IL‐6 around the time of the first immunization, on days −1 to 2, completely restoring disease susceptibility, showing that the requirement for IL‐6 coincides with primary immunization. Examining APC populations in the lymph node draining the immunization site evidenced the contribution of IL‐6 to the CFA‐dependence of EAM was through controlling local dendritic cell (DC) trafficking. CONCLUSIONS: CFA used with myocarditogenic peptide twice is required to induce EAM in both A/J and Balb/c mice. Although IFA and TiterMax induce antibody responses, only CFA preferentially induced autoantigen‐specific responses. CFA expands monocytes in the heart and in the spleen. IL‐6 signaling is required during short window around primary immunization to induce EAM. In addition, IL‐6 deficient mice resistance to EAM could be reversed by injecting IL‐6 around first immunization. IL‐6 expands dendritic cell and monocytic populations and ultimately leads to a robust T‐cell driven immune response in CFA immunized mice.
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spelling pubmed-54181342017-05-05 Complete Freund's adjuvant induces experimental autoimmune myocarditis by enhancing IL‐6 production during initiation of the immune response Fontes, Jillian A. Barin, Jobert G. Talor, Monica V. Stickel, Natalie Schaub, Julie Rose, Noel R. Čiháková, Daniela Immun Inflamm Dis Original Research INTRODUCTION: Complete Freund's Adjuvant (CFA) emulsified with an antigen is a widely used method to induce autoimmune disease in animal models, yet the contribution of CFA to the immune response is not well understood. We compared the effectiveness of CFA with Incomplete Freund's Adjuvant (IFA) or TiterMax Gold Adjuvant (TMax) in experimental autoimmune myocarditis (EAM) in male mice. METHODS: EAM was induced in A/J, BALB/c, and IL6KO BALB/c male mice by injection of the myocarditogenic peptide in CFA, IFA, or TMax on days 0 and 7. EAM severity was analyzed by histology on day 21. In addition, specific flow cytometry outcomes were evaluated on day 21. RESULTS: Only mice immunized with CFA and myocarditogenic peptide on both days 0 and 7 developed substantial myocarditis as measured by histology. We observed a significantly increased level of IL6 in the spleen 3 days after CFA immunization. In the spleen and heart on day 21, there was an expansion of myeloid cells in CFA‐immunized mice, as compared to IFA or TMax‐immunized animals. Recombinant IL‐6 at the time of IFA immunization partially restored susceptibility of the mice to EAM. We also treated EAM‐resistant IL‐6 knockout mice with recombinant IL‐6 around the time of the first immunization, on days −1 to 2, completely restoring disease susceptibility, showing that the requirement for IL‐6 coincides with primary immunization. Examining APC populations in the lymph node draining the immunization site evidenced the contribution of IL‐6 to the CFA‐dependence of EAM was through controlling local dendritic cell (DC) trafficking. CONCLUSIONS: CFA used with myocarditogenic peptide twice is required to induce EAM in both A/J and Balb/c mice. Although IFA and TiterMax induce antibody responses, only CFA preferentially induced autoantigen‐specific responses. CFA expands monocytes in the heart and in the spleen. IL‐6 signaling is required during short window around primary immunization to induce EAM. In addition, IL‐6 deficient mice resistance to EAM could be reversed by injecting IL‐6 around first immunization. IL‐6 expands dendritic cell and monocytic populations and ultimately leads to a robust T‐cell driven immune response in CFA immunized mice. John Wiley and Sons Inc. 2017-03-13 /pmc/articles/PMC5418134/ /pubmed/28474508 http://dx.doi.org/10.1002/iid3.155 Text en © 2017 The Authors. Immunity, Inflammation and Disease Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Fontes, Jillian A.
Barin, Jobert G.
Talor, Monica V.
Stickel, Natalie
Schaub, Julie
Rose, Noel R.
Čiháková, Daniela
Complete Freund's adjuvant induces experimental autoimmune myocarditis by enhancing IL‐6 production during initiation of the immune response
title Complete Freund's adjuvant induces experimental autoimmune myocarditis by enhancing IL‐6 production during initiation of the immune response
title_full Complete Freund's adjuvant induces experimental autoimmune myocarditis by enhancing IL‐6 production during initiation of the immune response
title_fullStr Complete Freund's adjuvant induces experimental autoimmune myocarditis by enhancing IL‐6 production during initiation of the immune response
title_full_unstemmed Complete Freund's adjuvant induces experimental autoimmune myocarditis by enhancing IL‐6 production during initiation of the immune response
title_short Complete Freund's adjuvant induces experimental autoimmune myocarditis by enhancing IL‐6 production during initiation of the immune response
title_sort complete freund's adjuvant induces experimental autoimmune myocarditis by enhancing il‐6 production during initiation of the immune response
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5418134/
https://www.ncbi.nlm.nih.gov/pubmed/28474508
http://dx.doi.org/10.1002/iid3.155
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