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Co-existence of Methanogenesis and Sulfate Reduction with Common Substrates in Sulfate-Rich Estuarine Sediments

The competition between sulfate reducing bacteria and methanogens over common substrates has been proposed as a critical control for methane production. In this study, we examined the co-existence of methanogenesis and sulfate reduction with shared substrates over a large range of sulfate concentrat...

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Autores principales: Sela-Adler, Michal, Ronen, Zeev, Herut, Barak, Antler, Gilad, Vigderovich, Hanni, Eckert, Werner, Sivan, Orit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5418336/
https://www.ncbi.nlm.nih.gov/pubmed/28529500
http://dx.doi.org/10.3389/fmicb.2017.00766
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author Sela-Adler, Michal
Ronen, Zeev
Herut, Barak
Antler, Gilad
Vigderovich, Hanni
Eckert, Werner
Sivan, Orit
author_facet Sela-Adler, Michal
Ronen, Zeev
Herut, Barak
Antler, Gilad
Vigderovich, Hanni
Eckert, Werner
Sivan, Orit
author_sort Sela-Adler, Michal
collection PubMed
description The competition between sulfate reducing bacteria and methanogens over common substrates has been proposed as a critical control for methane production. In this study, we examined the co-existence of methanogenesis and sulfate reduction with shared substrates over a large range of sulfate concentrations and rates of sulfate reduction in estuarine systems, where these processes are the key terminal sink for organic carbon. Incubation experiments were carried out with sediment samples from the sulfate-methane transition zone of the Yarqon (Israel) estuary with different substrates and inhibitors along a sulfate concentrations gradient from 1 to 10 mM. The results show that methanogenesis and sulfate reduction can co-exist while the microbes share substrates over the tested range of sulfate concentrations and at sulfate reduction rates up to 680 μmol L(-1) day(-1). Rates of methanogenesis were two orders of magnitude lower than rates of sulfate reduction in incubations with acetate and lactate, suggesting a higher affinity of sulfate reducing bacteria for the available substrates. The co-existence of both processes was also confirmed by the isotopic signatures of δ(34)S in the residual sulfate and that of δ(13)C of methane and dissolved inorganic carbon. Copy numbers of dsrA and mcrA genes supported the dominance of sulfate reduction over methanogenesis, while showing also the ability of methanogens to grow under high sulfate concentration and in the presence of active sulfate reduction.
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spelling pubmed-54183362017-05-19 Co-existence of Methanogenesis and Sulfate Reduction with Common Substrates in Sulfate-Rich Estuarine Sediments Sela-Adler, Michal Ronen, Zeev Herut, Barak Antler, Gilad Vigderovich, Hanni Eckert, Werner Sivan, Orit Front Microbiol Microbiology The competition between sulfate reducing bacteria and methanogens over common substrates has been proposed as a critical control for methane production. In this study, we examined the co-existence of methanogenesis and sulfate reduction with shared substrates over a large range of sulfate concentrations and rates of sulfate reduction in estuarine systems, where these processes are the key terminal sink for organic carbon. Incubation experiments were carried out with sediment samples from the sulfate-methane transition zone of the Yarqon (Israel) estuary with different substrates and inhibitors along a sulfate concentrations gradient from 1 to 10 mM. The results show that methanogenesis and sulfate reduction can co-exist while the microbes share substrates over the tested range of sulfate concentrations and at sulfate reduction rates up to 680 μmol L(-1) day(-1). Rates of methanogenesis were two orders of magnitude lower than rates of sulfate reduction in incubations with acetate and lactate, suggesting a higher affinity of sulfate reducing bacteria for the available substrates. The co-existence of both processes was also confirmed by the isotopic signatures of δ(34)S in the residual sulfate and that of δ(13)C of methane and dissolved inorganic carbon. Copy numbers of dsrA and mcrA genes supported the dominance of sulfate reduction over methanogenesis, while showing also the ability of methanogens to grow under high sulfate concentration and in the presence of active sulfate reduction. Frontiers Media S.A. 2017-05-05 /pmc/articles/PMC5418336/ /pubmed/28529500 http://dx.doi.org/10.3389/fmicb.2017.00766 Text en Copyright © 2017 Sela-Adler, Ronen, Herut, Antler, Vigderovich, Eckert and Sivan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Sela-Adler, Michal
Ronen, Zeev
Herut, Barak
Antler, Gilad
Vigderovich, Hanni
Eckert, Werner
Sivan, Orit
Co-existence of Methanogenesis and Sulfate Reduction with Common Substrates in Sulfate-Rich Estuarine Sediments
title Co-existence of Methanogenesis and Sulfate Reduction with Common Substrates in Sulfate-Rich Estuarine Sediments
title_full Co-existence of Methanogenesis and Sulfate Reduction with Common Substrates in Sulfate-Rich Estuarine Sediments
title_fullStr Co-existence of Methanogenesis and Sulfate Reduction with Common Substrates in Sulfate-Rich Estuarine Sediments
title_full_unstemmed Co-existence of Methanogenesis and Sulfate Reduction with Common Substrates in Sulfate-Rich Estuarine Sediments
title_short Co-existence of Methanogenesis and Sulfate Reduction with Common Substrates in Sulfate-Rich Estuarine Sediments
title_sort co-existence of methanogenesis and sulfate reduction with common substrates in sulfate-rich estuarine sediments
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5418336/
https://www.ncbi.nlm.nih.gov/pubmed/28529500
http://dx.doi.org/10.3389/fmicb.2017.00766
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