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Two Rab5 Homologs Are Essential for the Development and Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae
The rice blast fungus, Magnaporthe oryzae, infects many economically important cereal crops, particularly rice. It has emerged as an important model organism for studying the growth, development, and pathogenesis of filamentous fungi. RabGTPases are important molecular switches in regulation of intr...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5418346/ https://www.ncbi.nlm.nih.gov/pubmed/28529514 http://dx.doi.org/10.3389/fpls.2017.00620 |
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author | Yang, Cheng D. Dang, Xie Zheng, Hua W. Chen, Xiao F. Lin, Xiao L. Zhang, Dong M. Abubakar, Yakubu S. Chen, Xin Lu, Guodong Wang, Zonghua Li, Guangpu Zhou, Jie |
author_facet | Yang, Cheng D. Dang, Xie Zheng, Hua W. Chen, Xiao F. Lin, Xiao L. Zhang, Dong M. Abubakar, Yakubu S. Chen, Xin Lu, Guodong Wang, Zonghua Li, Guangpu Zhou, Jie |
author_sort | Yang, Cheng D. |
collection | PubMed |
description | The rice blast fungus, Magnaporthe oryzae, infects many economically important cereal crops, particularly rice. It has emerged as an important model organism for studying the growth, development, and pathogenesis of filamentous fungi. RabGTPases are important molecular switches in regulation of intracellular membrane trafficking in all eukaryotes. MoRab5A and MoRab5B are Rab5 homologs in M. oryzae, but their functions in the fungal development and pathogenicity are unknown. In this study, we have employed a genetic approach and demonstrated that both MoRab5A and MoRab5B are crucial for vegetative growth and development, conidiogenesis, melanin synthesis, vacuole fusion, endocytosis, sexual reproduction, and plant pathogenesis in M. oryzae. Moreover, both MoRab5A and MoRab5B show similar localization in hyphae and conidia. To further investigate possible functional redundancy between MoRab5A and MoRab5B, we overexpressed MoRAB5A and MoRAB5B, respectively, in MoRab5B:RNAi and MoRab5A:RNAi strains, but neither could rescue each other’s defects caused by the RNAi. Taken together, we conclude that both MoRab5A and MoRab5B are necessary for the development and pathogenesis of the rice blast fungus, while they may function independently. |
format | Online Article Text |
id | pubmed-5418346 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-54183462017-05-19 Two Rab5 Homologs Are Essential for the Development and Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae Yang, Cheng D. Dang, Xie Zheng, Hua W. Chen, Xiao F. Lin, Xiao L. Zhang, Dong M. Abubakar, Yakubu S. Chen, Xin Lu, Guodong Wang, Zonghua Li, Guangpu Zhou, Jie Front Plant Sci Plant Science The rice blast fungus, Magnaporthe oryzae, infects many economically important cereal crops, particularly rice. It has emerged as an important model organism for studying the growth, development, and pathogenesis of filamentous fungi. RabGTPases are important molecular switches in regulation of intracellular membrane trafficking in all eukaryotes. MoRab5A and MoRab5B are Rab5 homologs in M. oryzae, but their functions in the fungal development and pathogenicity are unknown. In this study, we have employed a genetic approach and demonstrated that both MoRab5A and MoRab5B are crucial for vegetative growth and development, conidiogenesis, melanin synthesis, vacuole fusion, endocytosis, sexual reproduction, and plant pathogenesis in M. oryzae. Moreover, both MoRab5A and MoRab5B show similar localization in hyphae and conidia. To further investigate possible functional redundancy between MoRab5A and MoRab5B, we overexpressed MoRAB5A and MoRAB5B, respectively, in MoRab5B:RNAi and MoRab5A:RNAi strains, but neither could rescue each other’s defects caused by the RNAi. Taken together, we conclude that both MoRab5A and MoRab5B are necessary for the development and pathogenesis of the rice blast fungus, while they may function independently. Frontiers Media S.A. 2017-05-05 /pmc/articles/PMC5418346/ /pubmed/28529514 http://dx.doi.org/10.3389/fpls.2017.00620 Text en Copyright © 2017 Yang, Dang, Zheng, Chen, Lin, Zhang, Abubakar, Chen, Lu, Wang, Li and Zhou. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Yang, Cheng D. Dang, Xie Zheng, Hua W. Chen, Xiao F. Lin, Xiao L. Zhang, Dong M. Abubakar, Yakubu S. Chen, Xin Lu, Guodong Wang, Zonghua Li, Guangpu Zhou, Jie Two Rab5 Homologs Are Essential for the Development and Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae |
title | Two Rab5 Homologs Are Essential for the Development and Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae |
title_full | Two Rab5 Homologs Are Essential for the Development and Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae |
title_fullStr | Two Rab5 Homologs Are Essential for the Development and Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae |
title_full_unstemmed | Two Rab5 Homologs Are Essential for the Development and Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae |
title_short | Two Rab5 Homologs Are Essential for the Development and Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae |
title_sort | two rab5 homologs are essential for the development and pathogenicity of the rice blast fungus magnaporthe oryzae |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5418346/ https://www.ncbi.nlm.nih.gov/pubmed/28529514 http://dx.doi.org/10.3389/fpls.2017.00620 |
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