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The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin

The existence of common mechanisms regulating organ regeneration is an intriguing concept. Here we report on a regulatory element that is transiently activated during heart and fin regeneration in zebrafish. This element contains a ctgfa upstream sequence, called careg, which is induced by TGFβ/Acti...

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Detalles Bibliográficos
Autores principales: Pfefferli, Catherine, Jaźwińska, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5418624/
https://www.ncbi.nlm.nih.gov/pubmed/28466843
http://dx.doi.org/10.1038/ncomms15151
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author Pfefferli, Catherine
Jaźwińska, Anna
author_facet Pfefferli, Catherine
Jaźwińska, Anna
author_sort Pfefferli, Catherine
collection PubMed
description The existence of common mechanisms regulating organ regeneration is an intriguing concept. Here we report on a regulatory element that is transiently activated during heart and fin regeneration in zebrafish. This element contains a ctgfa upstream sequence, called careg, which is induced by TGFβ/Activin-β signalling in the peri-injury zone of the myocardium and the fin mesenchyme. In addition, this reporter demarcates a primordial cardiac layer and intraray osteoblasts. Using genetic fate mapping, we show the regenerative competence of careg-expressing cells. The analysis of the heart reveals that the primordial cardiac layer is incompletely restored after cryoinjury, whereas trabecular and cortical cardiomyocytes contribute to myocardial regrowth. In regenerating fins, the activated mesenchyme of the stump gives rise to the blastema. Our findings provide evidence of a common regenerative programme in cardiomyocytes and mesenchyme that opens the possibility to further explore conserved mechanisms of the cellular plasticity in diverse vertebrate organs.
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spelling pubmed-54186242017-07-06 The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin Pfefferli, Catherine Jaźwińska, Anna Nat Commun Article The existence of common mechanisms regulating organ regeneration is an intriguing concept. Here we report on a regulatory element that is transiently activated during heart and fin regeneration in zebrafish. This element contains a ctgfa upstream sequence, called careg, which is induced by TGFβ/Activin-β signalling in the peri-injury zone of the myocardium and the fin mesenchyme. In addition, this reporter demarcates a primordial cardiac layer and intraray osteoblasts. Using genetic fate mapping, we show the regenerative competence of careg-expressing cells. The analysis of the heart reveals that the primordial cardiac layer is incompletely restored after cryoinjury, whereas trabecular and cortical cardiomyocytes contribute to myocardial regrowth. In regenerating fins, the activated mesenchyme of the stump gives rise to the blastema. Our findings provide evidence of a common regenerative programme in cardiomyocytes and mesenchyme that opens the possibility to further explore conserved mechanisms of the cellular plasticity in diverse vertebrate organs. Nature Publishing Group 2017-05-03 /pmc/articles/PMC5418624/ /pubmed/28466843 http://dx.doi.org/10.1038/ncomms15151 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Pfefferli, Catherine
Jaźwińska, Anna
The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin
title The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin
title_full The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin
title_fullStr The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin
title_full_unstemmed The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin
title_short The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin
title_sort careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5418624/
https://www.ncbi.nlm.nih.gov/pubmed/28466843
http://dx.doi.org/10.1038/ncomms15151
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