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Interaction between cytochrome P450 2A6 and Catechol-O-Methyltransferase genes and their association with smoking risk in young men
BACKGROUND: Although some effects of gene–gene interactions on nicotine–dopamine metabolism for smoking behavior have been reported, polymorphisms of cytochrome P450 (CYP) 2A6 and catechol-O-methyltransferase (COMT) have not been studied together to determine their effects on smokers. The aim of thi...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5418756/ https://www.ncbi.nlm.nih.gov/pubmed/28472995 http://dx.doi.org/10.1186/s12993-017-0127-2 |
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author | Ou, Wei-Chih Huang, Yi-Chin Huang, Chih-Ling Lin, Min-Hsuan Chen, Yi-Chun Chen, Yi-Ju Liu, Chen-Nu Chen, Mei-Chih Huang, Ching-Shan Chen, Pei-Lain |
author_facet | Ou, Wei-Chih Huang, Yi-Chin Huang, Chih-Ling Lin, Min-Hsuan Chen, Yi-Chun Chen, Yi-Ju Liu, Chen-Nu Chen, Mei-Chih Huang, Ching-Shan Chen, Pei-Lain |
author_sort | Ou, Wei-Chih |
collection | PubMed |
description | BACKGROUND: Although some effects of gene–gene interactions on nicotine–dopamine metabolism for smoking behavior have been reported, polymorphisms of cytochrome P450 (CYP) 2A6 and catechol-O-methyltransferase (COMT) have not been studied together to determine their effects on smokers. The aim of this study was to investigate the effects of the interaction between the CYP 2A6 and COMT genes on smoking behavior in young Taiwanese men. RESULTS: A self-report questionnaire regarding smoking status was administered to 500 young men. Polymorphisms of the CYP 2A6 and COMT genes as well as urinary nicotine and urinary cotinine levels were determined. The odds ratio for starting smoking was significantly lower in subjects carrying a CYP2A6 low activity/variant COMT rs4680 genotype than in those possessing a CYP2A6 wild-type/variant COMT rs4680 genotype (0.44, 95% confidence interval = 0.19–0.98, P = 0.043). Comparisons of Fagerstrom Test for Nicotine Dependence (FTND), Physiological Cigarette Dependence Scale (PCDS), and Cigarette Withdrawal symptoms (CWS-21) among the smokers with different CYP2A6/COMT polymorphisms were not significantly different. The adjusted urinary nicotine concentrations were not significantly different between the two groups carrying different genotypes. The adjusted urinary cotinine level was significantly different between the COMT rs4680 wild-type group and COMT rs4680 variant group [92.46 ng/μL vs. 118.24 ng/μL (median value), P = 0.041] and between the COMT rs4680 wild-type/COMT rs165599 variant group and COMT rs4680 variant/COMT rs165599 variant group (97.10 ng/μL vs. 122.18 ng/μL, P = 0.022). CONCLUSIONS: These findings suggest that a single nucleotide polymorphism (rs4680) of the COMT gene and the interaction between the CYP 2A6 and COMT genes affect smoking status in young Taiwanese men. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12993-017-0127-2) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5418756 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-54187562017-05-08 Interaction between cytochrome P450 2A6 and Catechol-O-Methyltransferase genes and their association with smoking risk in young men Ou, Wei-Chih Huang, Yi-Chin Huang, Chih-Ling Lin, Min-Hsuan Chen, Yi-Chun Chen, Yi-Ju Liu, Chen-Nu Chen, Mei-Chih Huang, Ching-Shan Chen, Pei-Lain Behav Brain Funct Research BACKGROUND: Although some effects of gene–gene interactions on nicotine–dopamine metabolism for smoking behavior have been reported, polymorphisms of cytochrome P450 (CYP) 2A6 and catechol-O-methyltransferase (COMT) have not been studied together to determine their effects on smokers. The aim of this study was to investigate the effects of the interaction between the CYP 2A6 and COMT genes on smoking behavior in young Taiwanese men. RESULTS: A self-report questionnaire regarding smoking status was administered to 500 young men. Polymorphisms of the CYP 2A6 and COMT genes as well as urinary nicotine and urinary cotinine levels were determined. The odds ratio for starting smoking was significantly lower in subjects carrying a CYP2A6 low activity/variant COMT rs4680 genotype than in those possessing a CYP2A6 wild-type/variant COMT rs4680 genotype (0.44, 95% confidence interval = 0.19–0.98, P = 0.043). Comparisons of Fagerstrom Test for Nicotine Dependence (FTND), Physiological Cigarette Dependence Scale (PCDS), and Cigarette Withdrawal symptoms (CWS-21) among the smokers with different CYP2A6/COMT polymorphisms were not significantly different. The adjusted urinary nicotine concentrations were not significantly different between the two groups carrying different genotypes. The adjusted urinary cotinine level was significantly different between the COMT rs4680 wild-type group and COMT rs4680 variant group [92.46 ng/μL vs. 118.24 ng/μL (median value), P = 0.041] and between the COMT rs4680 wild-type/COMT rs165599 variant group and COMT rs4680 variant/COMT rs165599 variant group (97.10 ng/μL vs. 122.18 ng/μL, P = 0.022). CONCLUSIONS: These findings suggest that a single nucleotide polymorphism (rs4680) of the COMT gene and the interaction between the CYP 2A6 and COMT genes affect smoking status in young Taiwanese men. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12993-017-0127-2) contains supplementary material, which is available to authorized users. BioMed Central 2017-05-04 /pmc/articles/PMC5418756/ /pubmed/28472995 http://dx.doi.org/10.1186/s12993-017-0127-2 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Ou, Wei-Chih Huang, Yi-Chin Huang, Chih-Ling Lin, Min-Hsuan Chen, Yi-Chun Chen, Yi-Ju Liu, Chen-Nu Chen, Mei-Chih Huang, Ching-Shan Chen, Pei-Lain Interaction between cytochrome P450 2A6 and Catechol-O-Methyltransferase genes and their association with smoking risk in young men |
title | Interaction between cytochrome P450 2A6 and Catechol-O-Methyltransferase genes and their association with smoking risk in young men |
title_full | Interaction between cytochrome P450 2A6 and Catechol-O-Methyltransferase genes and their association with smoking risk in young men |
title_fullStr | Interaction between cytochrome P450 2A6 and Catechol-O-Methyltransferase genes and their association with smoking risk in young men |
title_full_unstemmed | Interaction between cytochrome P450 2A6 and Catechol-O-Methyltransferase genes and their association with smoking risk in young men |
title_short | Interaction between cytochrome P450 2A6 and Catechol-O-Methyltransferase genes and their association with smoking risk in young men |
title_sort | interaction between cytochrome p450 2a6 and catechol-o-methyltransferase genes and their association with smoking risk in young men |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5418756/ https://www.ncbi.nlm.nih.gov/pubmed/28472995 http://dx.doi.org/10.1186/s12993-017-0127-2 |
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