Development of a monoclonal anti-ADAMTS-5 antibody that specifically blocks the interaction with LRP1

The potent aggrecanase ADAMTS-5 is constitutively secreted by chondrocytes, but it is rapidly endocytosed in normal cartilage via the cell surface endocytic receptor LRP1. Therefore it is difficult to detect the total ADAMTS-5 activity produced. In this study, we isolated a monoclonal anti-ADAMTS-5...

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Detalles Bibliográficos
Autores principales: Santamaria, Salvatore, Fedorov, Oleg, McCafferty, John, Murphy, Gillian, Dudhia, Jayesh, Nagase, Hideaki, Yamamoto, Kazuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Brief Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5419085/
https://www.ncbi.nlm.nih.gov/pubmed/28306378
http://dx.doi.org/10.1080/19420862.2017.1304341
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author Santamaria, Salvatore
Fedorov, Oleg
McCafferty, John
Murphy, Gillian
Dudhia, Jayesh
Nagase, Hideaki
Yamamoto, Kazuhiro
author_facet Santamaria, Salvatore
Fedorov, Oleg
McCafferty, John
Murphy, Gillian
Dudhia, Jayesh
Nagase, Hideaki
Yamamoto, Kazuhiro
author_sort Santamaria, Salvatore
collection PubMed
description The potent aggrecanase ADAMTS-5 is constitutively secreted by chondrocytes, but it is rapidly endocytosed in normal cartilage via the cell surface endocytic receptor LRP1. Therefore it is difficult to detect the total ADAMTS-5 activity produced. In this study, we isolated a monoclonal anti-ADAMTS-5 antibody 1B7 that blocks LRP1-mediated internalization without affecting the aggrecanolytic activity. Addition of 1B7 to cultured human chondrocytes revealed the full aggrecanolytic activity of ADAMTS-5 generated by the cells. 1B7 is a useful tool to estimate the ADAMTS-5 activity and to identify its potential roles in the tissues.
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spelling pubmed-54190852017-05-16 Development of a monoclonal anti-ADAMTS-5 antibody that specifically blocks the interaction with LRP1 Santamaria, Salvatore Fedorov, Oleg McCafferty, John Murphy, Gillian Dudhia, Jayesh Nagase, Hideaki Yamamoto, Kazuhiro MAbs Brief Reports The potent aggrecanase ADAMTS-5 is constitutively secreted by chondrocytes, but it is rapidly endocytosed in normal cartilage via the cell surface endocytic receptor LRP1. Therefore it is difficult to detect the total ADAMTS-5 activity produced. In this study, we isolated a monoclonal anti-ADAMTS-5 antibody 1B7 that blocks LRP1-mediated internalization without affecting the aggrecanolytic activity. Addition of 1B7 to cultured human chondrocytes revealed the full aggrecanolytic activity of ADAMTS-5 generated by the cells. 1B7 is a useful tool to estimate the ADAMTS-5 activity and to identify its potential roles in the tissues. Taylor & Francis 2017-03-17 /pmc/articles/PMC5419085/ /pubmed/28306378 http://dx.doi.org/10.1080/19420862.2017.1304341 Text en © 2017 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Brief Reports
Santamaria, Salvatore
Fedorov, Oleg
McCafferty, John
Murphy, Gillian
Dudhia, Jayesh
Nagase, Hideaki
Yamamoto, Kazuhiro
Development of a monoclonal anti-ADAMTS-5 antibody that specifically blocks the interaction with LRP1
title Development of a monoclonal anti-ADAMTS-5 antibody that specifically blocks the interaction with LRP1
title_full Development of a monoclonal anti-ADAMTS-5 antibody that specifically blocks the interaction with LRP1
title_fullStr Development of a monoclonal anti-ADAMTS-5 antibody that specifically blocks the interaction with LRP1
title_full_unstemmed Development of a monoclonal anti-ADAMTS-5 antibody that specifically blocks the interaction with LRP1
title_short Development of a monoclonal anti-ADAMTS-5 antibody that specifically blocks the interaction with LRP1
title_sort development of a monoclonal anti-adamts-5 antibody that specifically blocks the interaction with lrp1
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5419085/
https://www.ncbi.nlm.nih.gov/pubmed/28306378
http://dx.doi.org/10.1080/19420862.2017.1304341
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