An improved method with high sensitivity and low background in detecting low β-galactosidase expression in mouse embryos

LacZ is widely used as a reporter in studies of gene expression patterns. β-galactosidase, the product of LacZ gene, is usually detected by X-gal/FeCN staining. In X-gal/FeCN staining, β-galactosidase catalyzes X-gal to produce blue precipitates, which indicate the expression patterns of the gene of...

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Detalles Bibliográficos
Autores principales: Shen, Xiaopeng, Bao, Wenjing, Yu, Wei, Liang, Rui, Nguyen, Bao, Liu, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5419561/
https://www.ncbi.nlm.nih.gov/pubmed/28475610
http://dx.doi.org/10.1371/journal.pone.0176915

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5419561/
https://www.ncbi.nlm.nih.gov/pubmed/28475610
http://dx.doi.org/10.1371/journal.pone.0176915

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