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Control of the induction of type I interferon by Peste des petits ruminants virus

Peste des petits ruminants virus (PPRV) is a morbillivirus that produces clinical disease in goats and sheep. We have studied the induction of interferon-β (IFN-β) following infection of cultured cells with wild-type and vaccine strains of PPRV, and the effects of such infection with PPRV on the ind...

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Autores principales: Sanz Bernardo, Beatriz, Goodbourn, Stephen, Baron, Michael D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5419582/
https://www.ncbi.nlm.nih.gov/pubmed/28475628
http://dx.doi.org/10.1371/journal.pone.0177300
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author Sanz Bernardo, Beatriz
Goodbourn, Stephen
Baron, Michael D.
author_facet Sanz Bernardo, Beatriz
Goodbourn, Stephen
Baron, Michael D.
author_sort Sanz Bernardo, Beatriz
collection PubMed
description Peste des petits ruminants virus (PPRV) is a morbillivirus that produces clinical disease in goats and sheep. We have studied the induction of interferon-β (IFN-β) following infection of cultured cells with wild-type and vaccine strains of PPRV, and the effects of such infection with PPRV on the induction of IFN-β through both MDA-5 and RIG-I mediated pathways. Using both reporter assays and direct measurement of IFN-β mRNA, we have found that PPRV infection induces IFN-β only weakly and transiently, and the virus can actively block the induction of IFN-β. We have also generated mutant PPRV that lack expression of either of the viral accessory proteins (V&C) to characterize the role of these proteins in IFN-β induction during virus infection. Both PPRV_ΔV and PPRV_ΔC were defective in growth in cell culture, although in different ways. While the PPRV V protein bound to MDA-5 and, to a lesser extent, RIG-I, and over-expression of the V protein inhibited both IFN-β induction pathways, PPRV lacking V protein expression can still block IFN-β induction. In contrast, PPRV C bound to neither MDA-5 nor RIG-I, but PPRV lacking C protein expression lost the ability to block both MDA-5 and RIG-I mediated activation of IFN-β. These results shed new light on the inhibition of the induction of IFN-β by PPRV.
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spelling pubmed-54195822017-05-14 Control of the induction of type I interferon by Peste des petits ruminants virus Sanz Bernardo, Beatriz Goodbourn, Stephen Baron, Michael D. PLoS One Research Article Peste des petits ruminants virus (PPRV) is a morbillivirus that produces clinical disease in goats and sheep. We have studied the induction of interferon-β (IFN-β) following infection of cultured cells with wild-type and vaccine strains of PPRV, and the effects of such infection with PPRV on the induction of IFN-β through both MDA-5 and RIG-I mediated pathways. Using both reporter assays and direct measurement of IFN-β mRNA, we have found that PPRV infection induces IFN-β only weakly and transiently, and the virus can actively block the induction of IFN-β. We have also generated mutant PPRV that lack expression of either of the viral accessory proteins (V&C) to characterize the role of these proteins in IFN-β induction during virus infection. Both PPRV_ΔV and PPRV_ΔC were defective in growth in cell culture, although in different ways. While the PPRV V protein bound to MDA-5 and, to a lesser extent, RIG-I, and over-expression of the V protein inhibited both IFN-β induction pathways, PPRV lacking V protein expression can still block IFN-β induction. In contrast, PPRV C bound to neither MDA-5 nor RIG-I, but PPRV lacking C protein expression lost the ability to block both MDA-5 and RIG-I mediated activation of IFN-β. These results shed new light on the inhibition of the induction of IFN-β by PPRV. Public Library of Science 2017-05-05 /pmc/articles/PMC5419582/ /pubmed/28475628 http://dx.doi.org/10.1371/journal.pone.0177300 Text en © 2017 Sanz Bernardo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Sanz Bernardo, Beatriz
Goodbourn, Stephen
Baron, Michael D.
Control of the induction of type I interferon by Peste des petits ruminants virus
title Control of the induction of type I interferon by Peste des petits ruminants virus
title_full Control of the induction of type I interferon by Peste des petits ruminants virus
title_fullStr Control of the induction of type I interferon by Peste des petits ruminants virus
title_full_unstemmed Control of the induction of type I interferon by Peste des petits ruminants virus
title_short Control of the induction of type I interferon by Peste des petits ruminants virus
title_sort control of the induction of type i interferon by peste des petits ruminants virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5419582/
https://www.ncbi.nlm.nih.gov/pubmed/28475628
http://dx.doi.org/10.1371/journal.pone.0177300
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