DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells

The actin cytoskeleton is essential for many fundamental biological processes, but tools to directly manipulate actin dynamics are limited to cell-permeable drugs precluding single cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce...

Descripción completa

Detalles Bibliográficos
Autores principales: Harterink, Martin, da Silva, Marta Esteves, Will, Lena, Turan, Julia, Ibrahim, Adiljan, Lang, Alexander E., van Battum, Eljo Y., Pasterkamp, R. Jeroen, Kapitein, Lukas C., Kudryashov, Dmitri, Barres, Ben A., Hoogenraad, Casper C., Zuchero, J. Bradley
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5419720/
https://www.ncbi.nlm.nih.gov/pubmed/28394337
http://dx.doi.org/10.1038/nmeth.4257
Descripción
Sumario:The actin cytoskeleton is essential for many fundamental biological processes, but tools to directly manipulate actin dynamics are limited to cell-permeable drugs precluding single cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce actin disassembly in eukaryotic cells. We demonstrate that DeActs are a universal tool for studying the actin cytoskeleton in single cells in culture, tissues, and multicellular organisms, including various neurodevelopmental model systems.

Ejemplares similares