DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells

The actin cytoskeleton is essential for many fundamental biological processes, but tools to directly manipulate actin dynamics are limited to cell-permeable drugs precluding single cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce...

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Autores principales: Harterink, Martin, da Silva, Marta Esteves, Will, Lena, Turan, Julia, Ibrahim, Adiljan, Lang, Alexander E., van Battum, Eljo Y., Pasterkamp, R. Jeroen, Kapitein, Lukas C., Kudryashov, Dmitri, Barres, Ben A., Hoogenraad, Casper C., Zuchero, J. Bradley
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5419720/
https://www.ncbi.nlm.nih.gov/pubmed/28394337
http://dx.doi.org/10.1038/nmeth.4257
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author Harterink, Martin
da Silva, Marta Esteves
Will, Lena
Turan, Julia
Ibrahim, Adiljan
Lang, Alexander E.
van Battum, Eljo Y.
Pasterkamp, R. Jeroen
Kapitein, Lukas C.
Kudryashov, Dmitri
Barres, Ben A.
Hoogenraad, Casper C.
Zuchero, J. Bradley
author_facet Harterink, Martin
da Silva, Marta Esteves
Will, Lena
Turan, Julia
Ibrahim, Adiljan
Lang, Alexander E.
van Battum, Eljo Y.
Pasterkamp, R. Jeroen
Kapitein, Lukas C.
Kudryashov, Dmitri
Barres, Ben A.
Hoogenraad, Casper C.
Zuchero, J. Bradley
author_sort Harterink, Martin
collection PubMed
description The actin cytoskeleton is essential for many fundamental biological processes, but tools to directly manipulate actin dynamics are limited to cell-permeable drugs precluding single cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce actin disassembly in eukaryotic cells. We demonstrate that DeActs are a universal tool for studying the actin cytoskeleton in single cells in culture, tissues, and multicellular organisms, including various neurodevelopmental model systems.
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spelling pubmed-54197202017-10-10 DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells Harterink, Martin da Silva, Marta Esteves Will, Lena Turan, Julia Ibrahim, Adiljan Lang, Alexander E. van Battum, Eljo Y. Pasterkamp, R. Jeroen Kapitein, Lukas C. Kudryashov, Dmitri Barres, Ben A. Hoogenraad, Casper C. Zuchero, J. Bradley Nat Methods Article The actin cytoskeleton is essential for many fundamental biological processes, but tools to directly manipulate actin dynamics are limited to cell-permeable drugs precluding single cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce actin disassembly in eukaryotic cells. We demonstrate that DeActs are a universal tool for studying the actin cytoskeleton in single cells in culture, tissues, and multicellular organisms, including various neurodevelopmental model systems. 2017-04-10 2017-05 /pmc/articles/PMC5419720/ /pubmed/28394337 http://dx.doi.org/10.1038/nmeth.4257 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Harterink, Martin
da Silva, Marta Esteves
Will, Lena
Turan, Julia
Ibrahim, Adiljan
Lang, Alexander E.
van Battum, Eljo Y.
Pasterkamp, R. Jeroen
Kapitein, Lukas C.
Kudryashov, Dmitri
Barres, Ben A.
Hoogenraad, Casper C.
Zuchero, J. Bradley
DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells
title DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells
title_full DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells
title_fullStr DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells
title_full_unstemmed DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells
title_short DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells
title_sort deacts: genetically encoded tools for perturbing the actin cytoskeleton in single cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5419720/
https://www.ncbi.nlm.nih.gov/pubmed/28394337
http://dx.doi.org/10.1038/nmeth.4257
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