Cargando…

Fast and Selective Plasmonic Serotonin Detection with Aptamer-Gold Nanoparticle Conjugates

Neurotransmitters detection is critical to understanding communication between the brain and peripheral tissue. Serotonin is a key neurotransmitter linked to a number of conditions, but a full understanding of its role in disease is still lacking. The development of fast and selective serotonin dete...

Descripción completa

Detalles Bibliográficos
Autores principales: Chávez, Jorge L., Hagen, Joshua A., Kelley-Loughnane, Nancy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5419794/
https://www.ncbi.nlm.nih.gov/pubmed/28346350
http://dx.doi.org/10.3390/s17040681
_version_ 1783234278381846528
author Chávez, Jorge L.
Hagen, Joshua A.
Kelley-Loughnane, Nancy
author_facet Chávez, Jorge L.
Hagen, Joshua A.
Kelley-Loughnane, Nancy
author_sort Chávez, Jorge L.
collection PubMed
description Neurotransmitters detection is critical to understanding communication between the brain and peripheral tissue. Serotonin is a key neurotransmitter linked to a number of conditions, but a full understanding of its role in disease is still lacking. The development of fast and selective serotonin detection platforms will provide researchers with tools to monitor serotonin in individuals before and after treatment for the condition of interest. Aptamer-gold nanoparticles conjugates that responded colorimetrically to serotonin with minimal response to its metabolite and other neurotransmitters were designed by simply adsorbing the DNA on the surface of AuNPs. A plasmonic assay for serotonin detection was designed with a response to biologically relevant serotonin levels. Importantly, the assay performance was not compromised when tested in filtered spiked fetal bovine serum as a mimic of biofluids. This work shows that these simple and stable Apt-AuNP conjugates are promising tools to develop fast assays for point-of-care and personalized diagnostics applications.
format Online
Article
Text
id pubmed-5419794
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-54197942017-05-12 Fast and Selective Plasmonic Serotonin Detection with Aptamer-Gold Nanoparticle Conjugates Chávez, Jorge L. Hagen, Joshua A. Kelley-Loughnane, Nancy Sensors (Basel) Article Neurotransmitters detection is critical to understanding communication between the brain and peripheral tissue. Serotonin is a key neurotransmitter linked to a number of conditions, but a full understanding of its role in disease is still lacking. The development of fast and selective serotonin detection platforms will provide researchers with tools to monitor serotonin in individuals before and after treatment for the condition of interest. Aptamer-gold nanoparticles conjugates that responded colorimetrically to serotonin with minimal response to its metabolite and other neurotransmitters were designed by simply adsorbing the DNA on the surface of AuNPs. A plasmonic assay for serotonin detection was designed with a response to biologically relevant serotonin levels. Importantly, the assay performance was not compromised when tested in filtered spiked fetal bovine serum as a mimic of biofluids. This work shows that these simple and stable Apt-AuNP conjugates are promising tools to develop fast assays for point-of-care and personalized diagnostics applications. MDPI 2017-03-25 /pmc/articles/PMC5419794/ /pubmed/28346350 http://dx.doi.org/10.3390/s17040681 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chávez, Jorge L.
Hagen, Joshua A.
Kelley-Loughnane, Nancy
Fast and Selective Plasmonic Serotonin Detection with Aptamer-Gold Nanoparticle Conjugates
title Fast and Selective Plasmonic Serotonin Detection with Aptamer-Gold Nanoparticle Conjugates
title_full Fast and Selective Plasmonic Serotonin Detection with Aptamer-Gold Nanoparticle Conjugates
title_fullStr Fast and Selective Plasmonic Serotonin Detection with Aptamer-Gold Nanoparticle Conjugates
title_full_unstemmed Fast and Selective Plasmonic Serotonin Detection with Aptamer-Gold Nanoparticle Conjugates
title_short Fast and Selective Plasmonic Serotonin Detection with Aptamer-Gold Nanoparticle Conjugates
title_sort fast and selective plasmonic serotonin detection with aptamer-gold nanoparticle conjugates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5419794/
https://www.ncbi.nlm.nih.gov/pubmed/28346350
http://dx.doi.org/10.3390/s17040681
work_keys_str_mv AT chavezjorgel fastandselectiveplasmonicserotonindetectionwithaptamergoldnanoparticleconjugates
AT hagenjoshuaa fastandselectiveplasmonicserotonindetectionwithaptamergoldnanoparticleconjugates
AT kelleyloughnanenancy fastandselectiveplasmonicserotonindetectionwithaptamergoldnanoparticleconjugates