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Fast and Selective Plasmonic Serotonin Detection with Aptamer-Gold Nanoparticle Conjugates
Neurotransmitters detection is critical to understanding communication between the brain and peripheral tissue. Serotonin is a key neurotransmitter linked to a number of conditions, but a full understanding of its role in disease is still lacking. The development of fast and selective serotonin dete...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5419794/ https://www.ncbi.nlm.nih.gov/pubmed/28346350 http://dx.doi.org/10.3390/s17040681 |
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author | Chávez, Jorge L. Hagen, Joshua A. Kelley-Loughnane, Nancy |
author_facet | Chávez, Jorge L. Hagen, Joshua A. Kelley-Loughnane, Nancy |
author_sort | Chávez, Jorge L. |
collection | PubMed |
description | Neurotransmitters detection is critical to understanding communication between the brain and peripheral tissue. Serotonin is a key neurotransmitter linked to a number of conditions, but a full understanding of its role in disease is still lacking. The development of fast and selective serotonin detection platforms will provide researchers with tools to monitor serotonin in individuals before and after treatment for the condition of interest. Aptamer-gold nanoparticles conjugates that responded colorimetrically to serotonin with minimal response to its metabolite and other neurotransmitters were designed by simply adsorbing the DNA on the surface of AuNPs. A plasmonic assay for serotonin detection was designed with a response to biologically relevant serotonin levels. Importantly, the assay performance was not compromised when tested in filtered spiked fetal bovine serum as a mimic of biofluids. This work shows that these simple and stable Apt-AuNP conjugates are promising tools to develop fast assays for point-of-care and personalized diagnostics applications. |
format | Online Article Text |
id | pubmed-5419794 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54197942017-05-12 Fast and Selective Plasmonic Serotonin Detection with Aptamer-Gold Nanoparticle Conjugates Chávez, Jorge L. Hagen, Joshua A. Kelley-Loughnane, Nancy Sensors (Basel) Article Neurotransmitters detection is critical to understanding communication between the brain and peripheral tissue. Serotonin is a key neurotransmitter linked to a number of conditions, but a full understanding of its role in disease is still lacking. The development of fast and selective serotonin detection platforms will provide researchers with tools to monitor serotonin in individuals before and after treatment for the condition of interest. Aptamer-gold nanoparticles conjugates that responded colorimetrically to serotonin with minimal response to its metabolite and other neurotransmitters were designed by simply adsorbing the DNA on the surface of AuNPs. A plasmonic assay for serotonin detection was designed with a response to biologically relevant serotonin levels. Importantly, the assay performance was not compromised when tested in filtered spiked fetal bovine serum as a mimic of biofluids. This work shows that these simple and stable Apt-AuNP conjugates are promising tools to develop fast assays for point-of-care and personalized diagnostics applications. MDPI 2017-03-25 /pmc/articles/PMC5419794/ /pubmed/28346350 http://dx.doi.org/10.3390/s17040681 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chávez, Jorge L. Hagen, Joshua A. Kelley-Loughnane, Nancy Fast and Selective Plasmonic Serotonin Detection with Aptamer-Gold Nanoparticle Conjugates |
title | Fast and Selective Plasmonic Serotonin Detection with Aptamer-Gold Nanoparticle Conjugates |
title_full | Fast and Selective Plasmonic Serotonin Detection with Aptamer-Gold Nanoparticle Conjugates |
title_fullStr | Fast and Selective Plasmonic Serotonin Detection with Aptamer-Gold Nanoparticle Conjugates |
title_full_unstemmed | Fast and Selective Plasmonic Serotonin Detection with Aptamer-Gold Nanoparticle Conjugates |
title_short | Fast and Selective Plasmonic Serotonin Detection with Aptamer-Gold Nanoparticle Conjugates |
title_sort | fast and selective plasmonic serotonin detection with aptamer-gold nanoparticle conjugates |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5419794/ https://www.ncbi.nlm.nih.gov/pubmed/28346350 http://dx.doi.org/10.3390/s17040681 |
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