H(2) production pathways in nutrient-replete mixotrophic Chlamydomonas cultures under low light. Response to the commentary article “On the pathways feeding the H(2) production process in nutrient-replete, hypoxic conditions,” by Alberto Scoma and Szilvia Z. Tóth

BACKGROUND: A recent Commentary article entitled “On the pathways feeding the H(2) production process in nutrient-replete, hypoxic conditions” by Dr. Scoma and Dr. Tóth, Biotechnology for Biofuels (2017), opened a very interesting debate about the H(2) production photosynthetic-linked pathways occurring in Chlamydomonas cultures grown in acetate-containing media and incubated under hypoxia/anoxia conditions. This Commentary article mainly focused on the results of our previous article “Low oxygen levels contribute to improve photohydrogen production in mixotrophic non-stressed Chlamydomonas cultures,” by Jurado-Oller et al., Biotechnology for Biofuels (7, 2015; 8:149). MAIN BODY: Here, we review some previous knowledge about the H(2) production pathways linked to photosynthesis in Chlamydomonas, especially focusing on the role of the PSII-dependent and -independent pathways in acetate-containing nutrient-replete cultures. The potential contributions of these pathways to H(2) production under anoxia/hypoxia are discussed. CONCLUSION: Despite the fact that the PSII inhibitor DCMU is broadly used to discern between the two different photosynthetic pathways operating under H(2) production conditions, its use may lead to distinctive conclusions depending on the growth conditions. The different potential sources of reductive power needed for the PSII-independent H(2) production in mixotrophic nutrient-replete cultures are a matter of debate and conclusive evidences are still missing.