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Castanea sativa Mill. bark extract exhibits chemopreventive properties triggering extrinsic apoptotic pathway in Jurkat cells
BACKGROUND: Chemoprevention represents the possibility to prevent, stop or reverse the cancerogenetic process. In this context the interest towards natural extracts and botanical drugs has constantly grown due to their phytochemical content. Castanea sativa Mill. (CSM) extracts showed to exert posit...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5420104/ https://www.ncbi.nlm.nih.gov/pubmed/28476162 http://dx.doi.org/10.1186/s12906-017-1756-6 |
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author | Lenzi, Monia Malaguti, Marco Cocchi, Veronica Hrelia, Silvana Hrelia, Patrizia |
author_facet | Lenzi, Monia Malaguti, Marco Cocchi, Veronica Hrelia, Silvana Hrelia, Patrizia |
author_sort | Lenzi, Monia |
collection | PubMed |
description | BACKGROUND: Chemoprevention represents the possibility to prevent, stop or reverse the cancerogenetic process. In this context the interest towards natural extracts and botanical drugs has constantly grown due to their phytochemical content. Castanea sativa Mill. (CSM) extracts showed to exert positive effect in the prevention/counteraction of chronic/degenerative diseases, therefore, we evaluated the potential chemopreventive effect of CSM bark extract. METHODS: Flow cytometry (FCM) analyses of Jurkat cells treated with CSM bark extract (0–500 μg·mL(−1)) for 24–72 h allowed evaluating its cytotoxicity and ability to induce apoptosis through the intrinsic or extrinsic pathways. Moreover, to evaluate CSM bark extract selectivity towards cancer cells, its cytotoxic and pro-apoptotic effect was also evaluated in human peripheral blood lymphocytes (PBL). RESULTS: CSM bark extract induced apoptosis in Jurkat cells in a dose- and time- dependent manner activating the extrinsic pathways as evidenced by the increase of activated caspase-8 positive cells. Moreover, IC(50) calculated after 24 h treatment resulted 304 and 128 μg·mL(−1) in PBL and Jurkat cells respectively. CONCLUSIONS: Our data suggest that CSM bark extract might be considered an interesting potential anti-cancer agent, since it induces apoptosis in cancer cells without appreciable cytotoxic effects on non-transformed cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12906-017-1756-6) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5420104 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-54201042017-05-08 Castanea sativa Mill. bark extract exhibits chemopreventive properties triggering extrinsic apoptotic pathway in Jurkat cells Lenzi, Monia Malaguti, Marco Cocchi, Veronica Hrelia, Silvana Hrelia, Patrizia BMC Complement Altern Med Research Article BACKGROUND: Chemoprevention represents the possibility to prevent, stop or reverse the cancerogenetic process. In this context the interest towards natural extracts and botanical drugs has constantly grown due to their phytochemical content. Castanea sativa Mill. (CSM) extracts showed to exert positive effect in the prevention/counteraction of chronic/degenerative diseases, therefore, we evaluated the potential chemopreventive effect of CSM bark extract. METHODS: Flow cytometry (FCM) analyses of Jurkat cells treated with CSM bark extract (0–500 μg·mL(−1)) for 24–72 h allowed evaluating its cytotoxicity and ability to induce apoptosis through the intrinsic or extrinsic pathways. Moreover, to evaluate CSM bark extract selectivity towards cancer cells, its cytotoxic and pro-apoptotic effect was also evaluated in human peripheral blood lymphocytes (PBL). RESULTS: CSM bark extract induced apoptosis in Jurkat cells in a dose- and time- dependent manner activating the extrinsic pathways as evidenced by the increase of activated caspase-8 positive cells. Moreover, IC(50) calculated after 24 h treatment resulted 304 and 128 μg·mL(−1) in PBL and Jurkat cells respectively. CONCLUSIONS: Our data suggest that CSM bark extract might be considered an interesting potential anti-cancer agent, since it induces apoptosis in cancer cells without appreciable cytotoxic effects on non-transformed cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12906-017-1756-6) contains supplementary material, which is available to authorized users. BioMed Central 2017-05-05 /pmc/articles/PMC5420104/ /pubmed/28476162 http://dx.doi.org/10.1186/s12906-017-1756-6 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Lenzi, Monia Malaguti, Marco Cocchi, Veronica Hrelia, Silvana Hrelia, Patrizia Castanea sativa Mill. bark extract exhibits chemopreventive properties triggering extrinsic apoptotic pathway in Jurkat cells |
title | Castanea sativa Mill. bark extract exhibits chemopreventive properties triggering extrinsic apoptotic pathway in Jurkat cells |
title_full | Castanea sativa Mill. bark extract exhibits chemopreventive properties triggering extrinsic apoptotic pathway in Jurkat cells |
title_fullStr | Castanea sativa Mill. bark extract exhibits chemopreventive properties triggering extrinsic apoptotic pathway in Jurkat cells |
title_full_unstemmed | Castanea sativa Mill. bark extract exhibits chemopreventive properties triggering extrinsic apoptotic pathway in Jurkat cells |
title_short | Castanea sativa Mill. bark extract exhibits chemopreventive properties triggering extrinsic apoptotic pathway in Jurkat cells |
title_sort | castanea sativa mill. bark extract exhibits chemopreventive properties triggering extrinsic apoptotic pathway in jurkat cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5420104/ https://www.ncbi.nlm.nih.gov/pubmed/28476162 http://dx.doi.org/10.1186/s12906-017-1756-6 |
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