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Genome-wide analysis and characterization of Aux/IAA family genes related to fruit ripening in papaya (Carica papaya L.)

BACKGROUND: Auxin/indole-3-acetic acid (Aux/IAA) family genes encode short-lived nuclear proteins that mediate the responses of auxin-related genes and are involved in several plant developmental and growth processes. However, how Aux/IAA genes function in the fruit development and ripening of papay...

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Autores principales: Liu, Kaidong, Yuan, Changchun, Feng, Shaoxian, Zhong, Shuting, Li, Haili, Zhong, Jundi, Shen, Chenjia, Liu, Jinxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5420106/
https://www.ncbi.nlm.nih.gov/pubmed/28476147
http://dx.doi.org/10.1186/s12864-017-3722-6
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author Liu, Kaidong
Yuan, Changchun
Feng, Shaoxian
Zhong, Shuting
Li, Haili
Zhong, Jundi
Shen, Chenjia
Liu, Jinxiang
author_facet Liu, Kaidong
Yuan, Changchun
Feng, Shaoxian
Zhong, Shuting
Li, Haili
Zhong, Jundi
Shen, Chenjia
Liu, Jinxiang
author_sort Liu, Kaidong
collection PubMed
description BACKGROUND: Auxin/indole-3-acetic acid (Aux/IAA) family genes encode short-lived nuclear proteins that mediate the responses of auxin-related genes and are involved in several plant developmental and growth processes. However, how Aux/IAA genes function in the fruit development and ripening of papaya (Carica papaya L.) is largely unknown. RESULTS: In this study, a comprehensive identification and a distinctive expression analysis of 18 C. papaya Aux/IAA (CpIAA) genes were performed using newly updated papaya reference genome data. The Aux/IAA gene family in papaya is slightly smaller than that in Arabidopsis, but all of the phylogenetic subfamilies are represented. Most of the CpIAA genes are responsive to various phytohormones and expressed in a tissues-specific manner. To understand the putative biological functions of the CpIAA genes involved in fruit development and ripening, quantitative real-time PCR was used to test the expression profiling of CpIAA genes at different stages. Furthermore, an IAA treatment significantly delayed the ripening process in papaya fruit at the early stages. The expression changes of CpIAA genes in ACC and 1-MCP treatments suggested a crosstalk between auxin and ethylene during the fruit ripening process of papaya. CONCLUSIONS: Our study provided comprehensive information on the Aux/IAA family in papaya, including gene structures, phylogenetic relationships and expression profiles. The involvement of CpIAA gene expression changes in fruit development and ripening gives us an opportunity to understand the roles of auxin signaling in the maturation of papaya reproductive organs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-017-3722-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-54201062017-05-08 Genome-wide analysis and characterization of Aux/IAA family genes related to fruit ripening in papaya (Carica papaya L.) Liu, Kaidong Yuan, Changchun Feng, Shaoxian Zhong, Shuting Li, Haili Zhong, Jundi Shen, Chenjia Liu, Jinxiang BMC Genomics Research Article BACKGROUND: Auxin/indole-3-acetic acid (Aux/IAA) family genes encode short-lived nuclear proteins that mediate the responses of auxin-related genes and are involved in several plant developmental and growth processes. However, how Aux/IAA genes function in the fruit development and ripening of papaya (Carica papaya L.) is largely unknown. RESULTS: In this study, a comprehensive identification and a distinctive expression analysis of 18 C. papaya Aux/IAA (CpIAA) genes were performed using newly updated papaya reference genome data. The Aux/IAA gene family in papaya is slightly smaller than that in Arabidopsis, but all of the phylogenetic subfamilies are represented. Most of the CpIAA genes are responsive to various phytohormones and expressed in a tissues-specific manner. To understand the putative biological functions of the CpIAA genes involved in fruit development and ripening, quantitative real-time PCR was used to test the expression profiling of CpIAA genes at different stages. Furthermore, an IAA treatment significantly delayed the ripening process in papaya fruit at the early stages. The expression changes of CpIAA genes in ACC and 1-MCP treatments suggested a crosstalk between auxin and ethylene during the fruit ripening process of papaya. CONCLUSIONS: Our study provided comprehensive information on the Aux/IAA family in papaya, including gene structures, phylogenetic relationships and expression profiles. The involvement of CpIAA gene expression changes in fruit development and ripening gives us an opportunity to understand the roles of auxin signaling in the maturation of papaya reproductive organs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-017-3722-6) contains supplementary material, which is available to authorized users. BioMed Central 2017-05-05 /pmc/articles/PMC5420106/ /pubmed/28476147 http://dx.doi.org/10.1186/s12864-017-3722-6 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Liu, Kaidong
Yuan, Changchun
Feng, Shaoxian
Zhong, Shuting
Li, Haili
Zhong, Jundi
Shen, Chenjia
Liu, Jinxiang
Genome-wide analysis and characterization of Aux/IAA family genes related to fruit ripening in papaya (Carica papaya L.)
title Genome-wide analysis and characterization of Aux/IAA family genes related to fruit ripening in papaya (Carica papaya L.)
title_full Genome-wide analysis and characterization of Aux/IAA family genes related to fruit ripening in papaya (Carica papaya L.)
title_fullStr Genome-wide analysis and characterization of Aux/IAA family genes related to fruit ripening in papaya (Carica papaya L.)
title_full_unstemmed Genome-wide analysis and characterization of Aux/IAA family genes related to fruit ripening in papaya (Carica papaya L.)
title_short Genome-wide analysis and characterization of Aux/IAA family genes related to fruit ripening in papaya (Carica papaya L.)
title_sort genome-wide analysis and characterization of aux/iaa family genes related to fruit ripening in papaya (carica papaya l.)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5420106/
https://www.ncbi.nlm.nih.gov/pubmed/28476147
http://dx.doi.org/10.1186/s12864-017-3722-6
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