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CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma
INTRODUCTION: Connective tissue growth factor (CTGF) mediates hypertrophy, proliferation, and extracellular matrix synthesis. Matrix metalloproteinase (MMP) plays a role in airway extracellular matrix remodeling. The correlation between CTGF and MMP in airway remodeling of asthma was unknown. This s...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5420622/ https://www.ncbi.nlm.nih.gov/pubmed/28507585 http://dx.doi.org/10.5114/aoms.2016.60371 |
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author | Lin, Sheng-Chieh Chou, Hsiu-Chu Chiang, Bor-Luen Chen, Chung-Ming |
author_facet | Lin, Sheng-Chieh Chou, Hsiu-Chu Chiang, Bor-Luen Chen, Chung-Ming |
author_sort | Lin, Sheng-Chieh |
collection | PubMed |
description | INTRODUCTION: Connective tissue growth factor (CTGF) mediates hypertrophy, proliferation, and extracellular matrix synthesis. Matrix metalloproteinase (MMP) plays a role in airway extracellular matrix remodeling. The correlation between CTGF and MMP in airway remodeling of asthma was unknown. This study investigated lung CTGF expression and its correlation with MMP and airway structural changes in a murine model of asthma. MATERIAL AND METHODS: Female BALB/c mice were sensitized and challenged by intraperitoneal injections and intranasal phosphate-buffered saline (PBS) or ovalbumin (OVA). Airway responsiveness and serum OVA-specific IgE were measured. Airway structural changes were quantified by morphometric analysis. Differential cell counts and MMP-2, MMP-9, and tissue inhibitor of metalloproteinase (TIMP)-1 were evaluated in bronchoalveolar lavage fluid (BALF). Lung CTGF was determined by Western blot. RESULTS: Serum OVA-specific IgE level and airway responsiveness in enhanced pause (Penh) is significantly higher in sensitized mice challenged with OVA compared to PBS-challenged mice. MMP-2, MMP-9, and TIMP-1 in BALF were significantly higher in OVA mice. Airway structural changes of animals’ lungs with OVA challenge showed increased thickness of the smooth muscle layer and numbers of Goblet cells and inflammatory cells and eosinophils near airways and perivascular areas. Lung CTGF expression significantly increased in OVA-challenged mice. CTGF expressions positively correlated with MMP-9 (r = 0.677, p < 0.05), TIMP-1 (r = 0.574, p < 0.05) and thickness of the smooth muscle layer (r = 0.499, p < 0.05). CONCLUSIONS: This study indicates that CTGF upregulation correlates with MMP-9, probably involved in the pathogenesis of airway remodeling of asthma. |
format | Online Article Text |
id | pubmed-5420622 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-54206222017-05-15 CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma Lin, Sheng-Chieh Chou, Hsiu-Chu Chiang, Bor-Luen Chen, Chung-Ming Arch Med Sci Experimental Research INTRODUCTION: Connective tissue growth factor (CTGF) mediates hypertrophy, proliferation, and extracellular matrix synthesis. Matrix metalloproteinase (MMP) plays a role in airway extracellular matrix remodeling. The correlation between CTGF and MMP in airway remodeling of asthma was unknown. This study investigated lung CTGF expression and its correlation with MMP and airway structural changes in a murine model of asthma. MATERIAL AND METHODS: Female BALB/c mice were sensitized and challenged by intraperitoneal injections and intranasal phosphate-buffered saline (PBS) or ovalbumin (OVA). Airway responsiveness and serum OVA-specific IgE were measured. Airway structural changes were quantified by morphometric analysis. Differential cell counts and MMP-2, MMP-9, and tissue inhibitor of metalloproteinase (TIMP)-1 were evaluated in bronchoalveolar lavage fluid (BALF). Lung CTGF was determined by Western blot. RESULTS: Serum OVA-specific IgE level and airway responsiveness in enhanced pause (Penh) is significantly higher in sensitized mice challenged with OVA compared to PBS-challenged mice. MMP-2, MMP-9, and TIMP-1 in BALF were significantly higher in OVA mice. Airway structural changes of animals’ lungs with OVA challenge showed increased thickness of the smooth muscle layer and numbers of Goblet cells and inflammatory cells and eosinophils near airways and perivascular areas. Lung CTGF expression significantly increased in OVA-challenged mice. CTGF expressions positively correlated with MMP-9 (r = 0.677, p < 0.05), TIMP-1 (r = 0.574, p < 0.05) and thickness of the smooth muscle layer (r = 0.499, p < 0.05). CONCLUSIONS: This study indicates that CTGF upregulation correlates with MMP-9, probably involved in the pathogenesis of airway remodeling of asthma. Termedia Publishing House 2016-06-06 2017-04-01 /pmc/articles/PMC5420622/ /pubmed/28507585 http://dx.doi.org/10.5114/aoms.2016.60371 Text en Copyright: © 2016 Termedia & Banach http://creativecommons.org/licenses/by-nc-sa/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license. |
spellingShingle | Experimental Research Lin, Sheng-Chieh Chou, Hsiu-Chu Chiang, Bor-Luen Chen, Chung-Ming CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma |
title | CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma |
title_full | CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma |
title_fullStr | CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma |
title_full_unstemmed | CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma |
title_short | CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma |
title_sort | ctgf upregulation correlates with mmp-9 level in airway remodeling in a murine model of asthma |
topic | Experimental Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5420622/ https://www.ncbi.nlm.nih.gov/pubmed/28507585 http://dx.doi.org/10.5114/aoms.2016.60371 |
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