Cargando…

Expression of FGFR3 Protein and Gene Amplification in Urinary Bladder Lesions in Relation to Schistosomiasis

BACKGROUND: Bladder cancer represents the fifth most common malignancy worldwide and a major cause of cancer-related morbidity and death. Incidence and mortality rates have remained relatively constant over the past four decades. Urothelial bladder cancers have identified multiple risk factors. AIM:...

Descripción completa

Detalles Bibliográficos
Autores principales: Hammam, Olfat, Aboushousha, Tarek, El-Hindawi, Ali, Khairy, Hosni, Khalil, Heba, Kamel, Amira, Akl, Maha, Abdel-Hady, Ahmed, Magdy, Mona, Badawy, Mohamed, Kholy, Amr El, Osili, Khalid Al, Kamel, Nora, Anis, Shady, Leithy, Tarek El
Formato: Online Artículo Texto
Lenguaje:English
Publicado: ID Design 2012/DOOEL Skopje 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5420767/
https://www.ncbi.nlm.nih.gov/pubmed/28507621
http://dx.doi.org/10.3889/oamjms.2017.048
_version_ 1783234460193390592
author Hammam, Olfat
Aboushousha, Tarek
El-Hindawi, Ali
Khairy, Hosni
Khalil, Heba
Kamel, Amira
Akl, Maha
Abdel-Hady, Ahmed
Magdy, Mona
Badawy, Mohamed
Kholy, Amr El
Osili, Khalid Al
Kamel, Nora
Anis, Shady
Leithy, Tarek El
author_facet Hammam, Olfat
Aboushousha, Tarek
El-Hindawi, Ali
Khairy, Hosni
Khalil, Heba
Kamel, Amira
Akl, Maha
Abdel-Hady, Ahmed
Magdy, Mona
Badawy, Mohamed
Kholy, Amr El
Osili, Khalid Al
Kamel, Nora
Anis, Shady
Leithy, Tarek El
author_sort Hammam, Olfat
collection PubMed
description BACKGROUND: Bladder cancer represents the fifth most common malignancy worldwide and a major cause of cancer-related morbidity and death. Incidence and mortality rates have remained relatively constant over the past four decades. Urothelial bladder cancers have identified multiple risk factors. AIM: We aimed at evaluating the expression of the FGFR3 protein and gene amplification in the urothelial cells of neoplastic and non-neoplastic urothelial lesions of the urinary bladder, and correlation with tumour grade, stage and associated bilharziasis. MATERIAL AND METHODS: One hundred and five different urinary bladder lesions were studied, including 15 cystitis cases (9 bilharzial and 6 non-bilharzial cystitides), 75 urothelial carcinoma cases (18 bilharzial associated and 57 non-bilharzial associated) and 15 squamous cell carcinoma associated with bilharziasis, beside 5 control cases. Data concerning age, sex, tumour grade, stage, and associated bilharziasis were obtained. Each case was studied for FGFR3 expression, and FISH technique was applied on forty malignant cases that show high protein expression. RESULTS: The highest incidence of cystitis was in the fourth decade while of bladder cancer was in the seventh decade. Tumour grade was correlated significantly with tumour stage. FGFR3 correlates significantly with tumour grade, stage and with a bilharzial infestation. FGFR3 gene amplification was reported mainly in low grade and NNMBIC tumours. CONCLUSIONS: FGFR3 overexpression in malignant cases was significantly higher than in chronic cystitis. FGFR3 gene amplification was reported mainly in low grade and NNMBIC tumours. FGFR3 may be further studied as a subject for target therapy of bladder cancer.
format Online
Article
Text
id pubmed-5420767
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher ID Design 2012/DOOEL Skopje
record_format MEDLINE/PubMed
spelling pubmed-54207672017-05-15 Expression of FGFR3 Protein and Gene Amplification in Urinary Bladder Lesions in Relation to Schistosomiasis Hammam, Olfat Aboushousha, Tarek El-Hindawi, Ali Khairy, Hosni Khalil, Heba Kamel, Amira Akl, Maha Abdel-Hady, Ahmed Magdy, Mona Badawy, Mohamed Kholy, Amr El Osili, Khalid Al Kamel, Nora Anis, Shady Leithy, Tarek El Open Access Maced J Med Sci Basic Science BACKGROUND: Bladder cancer represents the fifth most common malignancy worldwide and a major cause of cancer-related morbidity and death. Incidence and mortality rates have remained relatively constant over the past four decades. Urothelial bladder cancers have identified multiple risk factors. AIM: We aimed at evaluating the expression of the FGFR3 protein and gene amplification in the urothelial cells of neoplastic and non-neoplastic urothelial lesions of the urinary bladder, and correlation with tumour grade, stage and associated bilharziasis. MATERIAL AND METHODS: One hundred and five different urinary bladder lesions were studied, including 15 cystitis cases (9 bilharzial and 6 non-bilharzial cystitides), 75 urothelial carcinoma cases (18 bilharzial associated and 57 non-bilharzial associated) and 15 squamous cell carcinoma associated with bilharziasis, beside 5 control cases. Data concerning age, sex, tumour grade, stage, and associated bilharziasis were obtained. Each case was studied for FGFR3 expression, and FISH technique was applied on forty malignant cases that show high protein expression. RESULTS: The highest incidence of cystitis was in the fourth decade while of bladder cancer was in the seventh decade. Tumour grade was correlated significantly with tumour stage. FGFR3 correlates significantly with tumour grade, stage and with a bilharzial infestation. FGFR3 gene amplification was reported mainly in low grade and NNMBIC tumours. CONCLUSIONS: FGFR3 overexpression in malignant cases was significantly higher than in chronic cystitis. FGFR3 gene amplification was reported mainly in low grade and NNMBIC tumours. FGFR3 may be further studied as a subject for target therapy of bladder cancer. ID Design 2012/DOOEL Skopje 2017-04-08 /pmc/articles/PMC5420767/ /pubmed/28507621 http://dx.doi.org/10.3889/oamjms.2017.048 Text en Copyright: © 2017 Olfat Hammam, Tarek Aboushousha, Ali El-Hindawi, Hosni Khairy, Heba Khalil, Amira Kamel, Maha Akl, Ahmed Abdel-Hady, Mona Magdy, Mohamed Badawy, Amr El Kholy, Khalid Al Osili, Nora Kamel, Shady Anis, Tarek El Leithy. http://creativecommons.org/licenses/CC BY-NC/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
spellingShingle Basic Science
Hammam, Olfat
Aboushousha, Tarek
El-Hindawi, Ali
Khairy, Hosni
Khalil, Heba
Kamel, Amira
Akl, Maha
Abdel-Hady, Ahmed
Magdy, Mona
Badawy, Mohamed
Kholy, Amr El
Osili, Khalid Al
Kamel, Nora
Anis, Shady
Leithy, Tarek El
Expression of FGFR3 Protein and Gene Amplification in Urinary Bladder Lesions in Relation to Schistosomiasis
title Expression of FGFR3 Protein and Gene Amplification in Urinary Bladder Lesions in Relation to Schistosomiasis
title_full Expression of FGFR3 Protein and Gene Amplification in Urinary Bladder Lesions in Relation to Schistosomiasis
title_fullStr Expression of FGFR3 Protein and Gene Amplification in Urinary Bladder Lesions in Relation to Schistosomiasis
title_full_unstemmed Expression of FGFR3 Protein and Gene Amplification in Urinary Bladder Lesions in Relation to Schistosomiasis
title_short Expression of FGFR3 Protein and Gene Amplification in Urinary Bladder Lesions in Relation to Schistosomiasis
title_sort expression of fgfr3 protein and gene amplification in urinary bladder lesions in relation to schistosomiasis
topic Basic Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5420767/
https://www.ncbi.nlm.nih.gov/pubmed/28507621
http://dx.doi.org/10.3889/oamjms.2017.048
work_keys_str_mv AT hammamolfat expressionoffgfr3proteinandgeneamplificationinurinarybladderlesionsinrelationtoschistosomiasis
AT aboushoushatarek expressionoffgfr3proteinandgeneamplificationinurinarybladderlesionsinrelationtoschistosomiasis
AT elhindawiali expressionoffgfr3proteinandgeneamplificationinurinarybladderlesionsinrelationtoschistosomiasis
AT khairyhosni expressionoffgfr3proteinandgeneamplificationinurinarybladderlesionsinrelationtoschistosomiasis
AT khalilheba expressionoffgfr3proteinandgeneamplificationinurinarybladderlesionsinrelationtoschistosomiasis
AT kamelamira expressionoffgfr3proteinandgeneamplificationinurinarybladderlesionsinrelationtoschistosomiasis
AT aklmaha expressionoffgfr3proteinandgeneamplificationinurinarybladderlesionsinrelationtoschistosomiasis
AT abdelhadyahmed expressionoffgfr3proteinandgeneamplificationinurinarybladderlesionsinrelationtoschistosomiasis
AT magdymona expressionoffgfr3proteinandgeneamplificationinurinarybladderlesionsinrelationtoschistosomiasis
AT badawymohamed expressionoffgfr3proteinandgeneamplificationinurinarybladderlesionsinrelationtoschistosomiasis
AT kholyamrel expressionoffgfr3proteinandgeneamplificationinurinarybladderlesionsinrelationtoschistosomiasis
AT osilikhalidal expressionoffgfr3proteinandgeneamplificationinurinarybladderlesionsinrelationtoschistosomiasis
AT kamelnora expressionoffgfr3proteinandgeneamplificationinurinarybladderlesionsinrelationtoschistosomiasis
AT anisshady expressionoffgfr3proteinandgeneamplificationinurinarybladderlesionsinrelationtoschistosomiasis
AT leithytarekel expressionoffgfr3proteinandgeneamplificationinurinarybladderlesionsinrelationtoschistosomiasis