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Salivary Immunoglobulin Gene Expression in Patients with Caries
BACKGROUND: Immunoglobulins mediate the host’s humoral immune response are expressed in saliva. AIM: To quantify the FcαR, FcγRIIB, and FcαμR gene expression in the saliva of Mexican patients with caries in mixed and permanent dentition. SUBJECTS AND METHODS: This was a comparative cross-sectional s...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
ID Design 2012/DOOEL Skopje
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5420781/ https://www.ncbi.nlm.nih.gov/pubmed/28507635 http://dx.doi.org/10.3889/oamjms.2017.028 |
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author | Santín, Gema Regina Guadarrama Salgado, Angel Visoso Bastida, Norma Margarita Montiel Gómez, Isaías de la Rosa Benítez, Jonnathan Guadalupe Santillán Zerón, Hugo Mendieta |
author_facet | Santín, Gema Regina Guadarrama Salgado, Angel Visoso Bastida, Norma Margarita Montiel Gómez, Isaías de la Rosa Benítez, Jonnathan Guadalupe Santillán Zerón, Hugo Mendieta |
author_sort | Santín, Gema Regina Guadarrama |
collection | PubMed |
description | BACKGROUND: Immunoglobulins mediate the host’s humoral immune response are expressed in saliva. AIM: To quantify the FcαR, FcγRIIB, and FcαμR gene expression in the saliva of Mexican patients with caries in mixed and permanent dentition. SUBJECTS AND METHODS: This was a comparative cross-sectional study. mRNA was isolated from 200 μL of saliva following the RNA III Tissue Fresh-frozen protocol of the MagNA Pure LC Instrument 2.0 (Roche Diagnostics GmbH, Nederland BV) and the FcαR, FcαμR and FcγRIIB were quantified through TaqMan Assays. RESULTS: One hundred individuals, 50 with mixed dentition and 50 with permanent dentition, were included in the study. Statistically, it was found a significant difference (p = 0.025) in the IgG (FcγRIIB) expression between the studied groups. CONCLUSION: Although we confirmed the existence of FcαR, FcγRIIB and FcαμR gene expression in saliva, only a significant difference in the expression of FcγRIIB between the mixed dentition and permanent dentition was found. |
format | Online Article Text |
id | pubmed-5420781 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | ID Design 2012/DOOEL Skopje |
record_format | MEDLINE/PubMed |
spelling | pubmed-54207812017-05-15 Salivary Immunoglobulin Gene Expression in Patients with Caries Santín, Gema Regina Guadarrama Salgado, Angel Visoso Bastida, Norma Margarita Montiel Gómez, Isaías de la Rosa Benítez, Jonnathan Guadalupe Santillán Zerón, Hugo Mendieta Open Access Maced J Med Sci Stomatology BACKGROUND: Immunoglobulins mediate the host’s humoral immune response are expressed in saliva. AIM: To quantify the FcαR, FcγRIIB, and FcαμR gene expression in the saliva of Mexican patients with caries in mixed and permanent dentition. SUBJECTS AND METHODS: This was a comparative cross-sectional study. mRNA was isolated from 200 μL of saliva following the RNA III Tissue Fresh-frozen protocol of the MagNA Pure LC Instrument 2.0 (Roche Diagnostics GmbH, Nederland BV) and the FcαR, FcαμR and FcγRIIB were quantified through TaqMan Assays. RESULTS: One hundred individuals, 50 with mixed dentition and 50 with permanent dentition, were included in the study. Statistically, it was found a significant difference (p = 0.025) in the IgG (FcγRIIB) expression between the studied groups. CONCLUSION: Although we confirmed the existence of FcαR, FcγRIIB and FcαμR gene expression in saliva, only a significant difference in the expression of FcγRIIB between the mixed dentition and permanent dentition was found. ID Design 2012/DOOEL Skopje 2017-03-03 /pmc/articles/PMC5420781/ /pubmed/28507635 http://dx.doi.org/10.3889/oamjms.2017.028 Text en Copyright: © 2017 Gema Regina Guadarrama Santín, Angel Visoso Salgado, Norma Margarita Montiel Bastida, Isaías de la Rosa Gómez, Jonnathan Guadalupe Santillán Benítez, Hugo Mendieta Zerón. http://creativecommons.org/licenses/CC BY-NC/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). |
spellingShingle | Stomatology Santín, Gema Regina Guadarrama Salgado, Angel Visoso Bastida, Norma Margarita Montiel Gómez, Isaías de la Rosa Benítez, Jonnathan Guadalupe Santillán Zerón, Hugo Mendieta Salivary Immunoglobulin Gene Expression in Patients with Caries |
title | Salivary Immunoglobulin Gene Expression in Patients with Caries |
title_full | Salivary Immunoglobulin Gene Expression in Patients with Caries |
title_fullStr | Salivary Immunoglobulin Gene Expression in Patients with Caries |
title_full_unstemmed | Salivary Immunoglobulin Gene Expression in Patients with Caries |
title_short | Salivary Immunoglobulin Gene Expression in Patients with Caries |
title_sort | salivary immunoglobulin gene expression in patients with caries |
topic | Stomatology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5420781/ https://www.ncbi.nlm.nih.gov/pubmed/28507635 http://dx.doi.org/10.3889/oamjms.2017.028 |
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