Cargando…

The microbiome of the lung and its extracellular vesicles in nonsmokers, healthy smokers and COPD patients

Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease, and bacterial infection plays a role in its pathogenesis. Bacteria secrete nanometer-sized extracellular vesicles (EVs), which may induce more immune dysfunction and inflammation than the bacteria themselves. We hypothes...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Hyun Jung, Kim, You-Sun, Kim, Kang-Hyun, Choi, Jun-Pyo, Kim, Yoon-Keun, Yun, Sunmi, Sharma, Lokesh, Dela Cruz, Charles S, Lee, Jae Seung, Oh, Yeon-Mok, Lee, Sang-Do, Lee, Sei Won
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5420800/
https://www.ncbi.nlm.nih.gov/pubmed/28408748
http://dx.doi.org/10.1038/emm.2017.7
_version_ 1783234468817928192
author Kim, Hyun Jung
Kim, You-Sun
Kim, Kang-Hyun
Choi, Jun-Pyo
Kim, Yoon-Keun
Yun, Sunmi
Sharma, Lokesh
Dela Cruz, Charles S
Lee, Jae Seung
Oh, Yeon-Mok
Lee, Sang-Do
Lee, Sei Won
author_facet Kim, Hyun Jung
Kim, You-Sun
Kim, Kang-Hyun
Choi, Jun-Pyo
Kim, Yoon-Keun
Yun, Sunmi
Sharma, Lokesh
Dela Cruz, Charles S
Lee, Jae Seung
Oh, Yeon-Mok
Lee, Sang-Do
Lee, Sei Won
author_sort Kim, Hyun Jung
collection PubMed
description Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease, and bacterial infection plays a role in its pathogenesis. Bacteria secrete nanometer-sized extracellular vesicles (EVs), which may induce more immune dysfunction and inflammation than the bacteria themselves. We hypothesized that the microbiome of lung EVs might have distinct characteristics depending on the presence of COPD and smoking status. We analyzed and compared the microbiomes of 13 nonsmokers with normal spirometry, 13 smokers with normal spirometry (healthy smokers) and 13 patients with COPD by using 16S ribosomal RNA gene sequencing of surgical lung tissue and lung EVs. Subjects were matched for age and sex in all groups and for smoking levels in the COPD and healthy smoker groups. Each group included 12 men and 1 woman with the same mean age of 65.5 years. In all groups, EVs consistently showed more operational taxonomic units (OTUs) than lung tissue. In the healthy smoker and COPD groups, EVs had a higher Shannon index and a lower Simpson index than lung tissue and this trend was more prominent in the COPD group. Principal component analysis (PCA) showed clusters based on sample type rather than participants' clinical characteristics. Stenotrophomonas, Propionibacterium and Alicyclobacillus were the most commonly found genera. Firmicutes were highly present in the EVs of the COPD group compared with other samples or groups. Our analysis of the lung microbiome revealed that the bacterial communities present in the EVs and in the COPD group possessed distinct characteristics with differences in the OTUs, diversity indexes and PCA clustering.
format Online
Article
Text
id pubmed-5420800
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-54208002017-05-19 The microbiome of the lung and its extracellular vesicles in nonsmokers, healthy smokers and COPD patients Kim, Hyun Jung Kim, You-Sun Kim, Kang-Hyun Choi, Jun-Pyo Kim, Yoon-Keun Yun, Sunmi Sharma, Lokesh Dela Cruz, Charles S Lee, Jae Seung Oh, Yeon-Mok Lee, Sang-Do Lee, Sei Won Exp Mol Med Original Article Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease, and bacterial infection plays a role in its pathogenesis. Bacteria secrete nanometer-sized extracellular vesicles (EVs), which may induce more immune dysfunction and inflammation than the bacteria themselves. We hypothesized that the microbiome of lung EVs might have distinct characteristics depending on the presence of COPD and smoking status. We analyzed and compared the microbiomes of 13 nonsmokers with normal spirometry, 13 smokers with normal spirometry (healthy smokers) and 13 patients with COPD by using 16S ribosomal RNA gene sequencing of surgical lung tissue and lung EVs. Subjects were matched for age and sex in all groups and for smoking levels in the COPD and healthy smoker groups. Each group included 12 men and 1 woman with the same mean age of 65.5 years. In all groups, EVs consistently showed more operational taxonomic units (OTUs) than lung tissue. In the healthy smoker and COPD groups, EVs had a higher Shannon index and a lower Simpson index than lung tissue and this trend was more prominent in the COPD group. Principal component analysis (PCA) showed clusters based on sample type rather than participants' clinical characteristics. Stenotrophomonas, Propionibacterium and Alicyclobacillus were the most commonly found genera. Firmicutes were highly present in the EVs of the COPD group compared with other samples or groups. Our analysis of the lung microbiome revealed that the bacterial communities present in the EVs and in the COPD group possessed distinct characteristics with differences in the OTUs, diversity indexes and PCA clustering. Nature Publishing Group 2017-04 2017-04-14 /pmc/articles/PMC5420800/ /pubmed/28408748 http://dx.doi.org/10.1038/emm.2017.7 Text en Copyright © 2017 The Author(s) http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Original Article
Kim, Hyun Jung
Kim, You-Sun
Kim, Kang-Hyun
Choi, Jun-Pyo
Kim, Yoon-Keun
Yun, Sunmi
Sharma, Lokesh
Dela Cruz, Charles S
Lee, Jae Seung
Oh, Yeon-Mok
Lee, Sang-Do
Lee, Sei Won
The microbiome of the lung and its extracellular vesicles in nonsmokers, healthy smokers and COPD patients
title The microbiome of the lung and its extracellular vesicles in nonsmokers, healthy smokers and COPD patients
title_full The microbiome of the lung and its extracellular vesicles in nonsmokers, healthy smokers and COPD patients
title_fullStr The microbiome of the lung and its extracellular vesicles in nonsmokers, healthy smokers and COPD patients
title_full_unstemmed The microbiome of the lung and its extracellular vesicles in nonsmokers, healthy smokers and COPD patients
title_short The microbiome of the lung and its extracellular vesicles in nonsmokers, healthy smokers and COPD patients
title_sort microbiome of the lung and its extracellular vesicles in nonsmokers, healthy smokers and copd patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5420800/
https://www.ncbi.nlm.nih.gov/pubmed/28408748
http://dx.doi.org/10.1038/emm.2017.7
work_keys_str_mv AT kimhyunjung themicrobiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT kimyousun themicrobiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT kimkanghyun themicrobiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT choijunpyo themicrobiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT kimyoonkeun themicrobiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT yunsunmi themicrobiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT sharmalokesh themicrobiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT delacruzcharless themicrobiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT leejaeseung themicrobiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT ohyeonmok themicrobiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT leesangdo themicrobiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT leeseiwon themicrobiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT kimhyunjung microbiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT kimyousun microbiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT kimkanghyun microbiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT choijunpyo microbiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT kimyoonkeun microbiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT yunsunmi microbiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT sharmalokesh microbiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT delacruzcharless microbiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT leejaeseung microbiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT ohyeonmok microbiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT leesangdo microbiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients
AT leeseiwon microbiomeofthelunganditsextracellularvesiclesinnonsmokershealthysmokersandcopdpatients