Cargando…

Bench-Top Fabrication of an All-PDMS Microfluidic Electrochemical Cell Sensor Integrating Micro/Nanostructured Electrodes

In recent years, efforts in the development of lab-on-a-chip (LoC) devices for point-of-care (PoC) applications have increased to bring affordable, portable, and sensitive diagnostics to the patients’ bedside. To reach this goal, research has shifted from using traditional microfabrication methods t...

Descripción completa

Detalles Bibliográficos
Autores principales: Saem, Sokunthearath, Zhu, Yujie, Luu, Helen, Moran-Mirabal, Jose
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5421692/
https://www.ncbi.nlm.nih.gov/pubmed/28362329
http://dx.doi.org/10.3390/s17040732
_version_ 1783234624640516096
author Saem, Sokunthearath
Zhu, Yujie
Luu, Helen
Moran-Mirabal, Jose
author_facet Saem, Sokunthearath
Zhu, Yujie
Luu, Helen
Moran-Mirabal, Jose
author_sort Saem, Sokunthearath
collection PubMed
description In recent years, efforts in the development of lab-on-a-chip (LoC) devices for point-of-care (PoC) applications have increased to bring affordable, portable, and sensitive diagnostics to the patients’ bedside. To reach this goal, research has shifted from using traditional microfabrication methods to more versatile, rapid, and low-cost options. This work focuses on the benchtop fabrication of a highly sensitive, fully transparent, and flexible poly (dimethylsiloxane) (PDMS) microfluidic (μF) electrochemical cell sensor. The μF device encapsulates 3D structured gold and platinum electrodes, fabricated using a shape-memory polymer shrinking method, which are used to set up an on-chip electrochemical cell. The PDMS to PDMS-structured electrode bonding protocol to fabricate the μF chip was optimized and found to have sufficient bond strength to withstand up to 100 mL/min flow rates. The sensing capabilities of the on-chip electrochemical cell were demonstrated by using cyclic voltammetry to monitor the adhesion of murine 3T3 fibroblasts in the presence of a redox reporter. The charge transfer across the working electrode was reduced upon cell adhesion, which was used as the detection mechanism, and allowed the detection of as few as 24 cells. The effective utilization of simple and low cost bench-top fabrication methods could accelerate the prototyping and development of LoC technologies and bring PoC diagnostics and personalized medicine to the patients’ bedside.
format Online
Article
Text
id pubmed-5421692
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-54216922017-05-12 Bench-Top Fabrication of an All-PDMS Microfluidic Electrochemical Cell Sensor Integrating Micro/Nanostructured Electrodes Saem, Sokunthearath Zhu, Yujie Luu, Helen Moran-Mirabal, Jose Sensors (Basel) Article In recent years, efforts in the development of lab-on-a-chip (LoC) devices for point-of-care (PoC) applications have increased to bring affordable, portable, and sensitive diagnostics to the patients’ bedside. To reach this goal, research has shifted from using traditional microfabrication methods to more versatile, rapid, and low-cost options. This work focuses on the benchtop fabrication of a highly sensitive, fully transparent, and flexible poly (dimethylsiloxane) (PDMS) microfluidic (μF) electrochemical cell sensor. The μF device encapsulates 3D structured gold and platinum electrodes, fabricated using a shape-memory polymer shrinking method, which are used to set up an on-chip electrochemical cell. The PDMS to PDMS-structured electrode bonding protocol to fabricate the μF chip was optimized and found to have sufficient bond strength to withstand up to 100 mL/min flow rates. The sensing capabilities of the on-chip electrochemical cell were demonstrated by using cyclic voltammetry to monitor the adhesion of murine 3T3 fibroblasts in the presence of a redox reporter. The charge transfer across the working electrode was reduced upon cell adhesion, which was used as the detection mechanism, and allowed the detection of as few as 24 cells. The effective utilization of simple and low cost bench-top fabrication methods could accelerate the prototyping and development of LoC technologies and bring PoC diagnostics and personalized medicine to the patients’ bedside. MDPI 2017-03-31 /pmc/articles/PMC5421692/ /pubmed/28362329 http://dx.doi.org/10.3390/s17040732 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saem, Sokunthearath
Zhu, Yujie
Luu, Helen
Moran-Mirabal, Jose
Bench-Top Fabrication of an All-PDMS Microfluidic Electrochemical Cell Sensor Integrating Micro/Nanostructured Electrodes
title Bench-Top Fabrication of an All-PDMS Microfluidic Electrochemical Cell Sensor Integrating Micro/Nanostructured Electrodes
title_full Bench-Top Fabrication of an All-PDMS Microfluidic Electrochemical Cell Sensor Integrating Micro/Nanostructured Electrodes
title_fullStr Bench-Top Fabrication of an All-PDMS Microfluidic Electrochemical Cell Sensor Integrating Micro/Nanostructured Electrodes
title_full_unstemmed Bench-Top Fabrication of an All-PDMS Microfluidic Electrochemical Cell Sensor Integrating Micro/Nanostructured Electrodes
title_short Bench-Top Fabrication of an All-PDMS Microfluidic Electrochemical Cell Sensor Integrating Micro/Nanostructured Electrodes
title_sort bench-top fabrication of an all-pdms microfluidic electrochemical cell sensor integrating micro/nanostructured electrodes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5421692/
https://www.ncbi.nlm.nih.gov/pubmed/28362329
http://dx.doi.org/10.3390/s17040732
work_keys_str_mv AT saemsokunthearath benchtopfabricationofanallpdmsmicrofluidicelectrochemicalcellsensorintegratingmicronanostructuredelectrodes
AT zhuyujie benchtopfabricationofanallpdmsmicrofluidicelectrochemicalcellsensorintegratingmicronanostructuredelectrodes
AT luuhelen benchtopfabricationofanallpdmsmicrofluidicelectrochemicalcellsensorintegratingmicronanostructuredelectrodes
AT moranmirabaljose benchtopfabricationofanallpdmsmicrofluidicelectrochemicalcellsensorintegratingmicronanostructuredelectrodes