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The role of VosA/VelB-activated developmental gene vadA in Aspergillus nidulans

The filamentous fungus Aspergillus nidulans primarily reproduces by forming asexual spores called conidia, the integrity of which is governed by the NF-κB type velvet regulators VosA and VelB. The VosA-VelB hetero-complex regulates the expression of spore-specific structural and regulatory genes dur...

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Autores principales: Park, Hee-Soo, Lee, Mi-Kyung, Kim, Sun Chang, Yu, Jae-Hyuk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5421774/
https://www.ncbi.nlm.nih.gov/pubmed/28481894
http://dx.doi.org/10.1371/journal.pone.0177099
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author Park, Hee-Soo
Lee, Mi-Kyung
Kim, Sun Chang
Yu, Jae-Hyuk
author_facet Park, Hee-Soo
Lee, Mi-Kyung
Kim, Sun Chang
Yu, Jae-Hyuk
author_sort Park, Hee-Soo
collection PubMed
description The filamentous fungus Aspergillus nidulans primarily reproduces by forming asexual spores called conidia, the integrity of which is governed by the NF-κB type velvet regulators VosA and VelB. The VosA-VelB hetero-complex regulates the expression of spore-specific structural and regulatory genes during conidiogenesis. Here, we characterize one of the VosA/VelB-activated developmental genes, called vadA, the expression of which in conidia requires activity of both VosA and VelB. VadA (AN5709) is predicted to be a 532-amino acid length fungal-specific protein with a highly conserved domain of unknown function (DUF) at the N-terminus. This DUF was found to be conserved in many Ascomycota and some Glomeromycota species, suggesting a potential evolutionarily conserved function of this domain in fungi. Deletion studies of vadA indicate that VadA is required for proper downregulation of brlA, fksA, and rodA, and for proper expression of tpsA and orlA during sporogenesis. Moreover, vadA null mutant conidia exhibit decreased trehalose content, but increased β(1,3)-glucan levels, lower viability, and reduced tolerance to oxidative stress. We further demonstrate that the vadA null mutant shows increased production of the mycotoxin sterigmatocystin. In summary, VadA is a dual-function novel regulator that controls development and secondary metabolism, and participates in bridging differentiation and viability of newly formed conidia in A. nidulans.
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spelling pubmed-54217742017-05-14 The role of VosA/VelB-activated developmental gene vadA in Aspergillus nidulans Park, Hee-Soo Lee, Mi-Kyung Kim, Sun Chang Yu, Jae-Hyuk PLoS One Research Article The filamentous fungus Aspergillus nidulans primarily reproduces by forming asexual spores called conidia, the integrity of which is governed by the NF-κB type velvet regulators VosA and VelB. The VosA-VelB hetero-complex regulates the expression of spore-specific structural and regulatory genes during conidiogenesis. Here, we characterize one of the VosA/VelB-activated developmental genes, called vadA, the expression of which in conidia requires activity of both VosA and VelB. VadA (AN5709) is predicted to be a 532-amino acid length fungal-specific protein with a highly conserved domain of unknown function (DUF) at the N-terminus. This DUF was found to be conserved in many Ascomycota and some Glomeromycota species, suggesting a potential evolutionarily conserved function of this domain in fungi. Deletion studies of vadA indicate that VadA is required for proper downregulation of brlA, fksA, and rodA, and for proper expression of tpsA and orlA during sporogenesis. Moreover, vadA null mutant conidia exhibit decreased trehalose content, but increased β(1,3)-glucan levels, lower viability, and reduced tolerance to oxidative stress. We further demonstrate that the vadA null mutant shows increased production of the mycotoxin sterigmatocystin. In summary, VadA is a dual-function novel regulator that controls development and secondary metabolism, and participates in bridging differentiation and viability of newly formed conidia in A. nidulans. Public Library of Science 2017-05-08 /pmc/articles/PMC5421774/ /pubmed/28481894 http://dx.doi.org/10.1371/journal.pone.0177099 Text en © 2017 Park et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Park, Hee-Soo
Lee, Mi-Kyung
Kim, Sun Chang
Yu, Jae-Hyuk
The role of VosA/VelB-activated developmental gene vadA in Aspergillus nidulans
title The role of VosA/VelB-activated developmental gene vadA in Aspergillus nidulans
title_full The role of VosA/VelB-activated developmental gene vadA in Aspergillus nidulans
title_fullStr The role of VosA/VelB-activated developmental gene vadA in Aspergillus nidulans
title_full_unstemmed The role of VosA/VelB-activated developmental gene vadA in Aspergillus nidulans
title_short The role of VosA/VelB-activated developmental gene vadA in Aspergillus nidulans
title_sort role of vosa/velb-activated developmental gene vada in aspergillus nidulans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5421774/
https://www.ncbi.nlm.nih.gov/pubmed/28481894
http://dx.doi.org/10.1371/journal.pone.0177099
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