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Adenovirus-vectored novel African Swine Fever Virus antigens elicit robust immune responses in swine

African Swine Fever Virus (ASFV) is a high-consequence transboundary animal pathogen that often causes hemorrhagic disease in swine with a case fatality rate close to 100%. Lack of treatment or vaccine for the disease makes it imperative that safe and efficacious vaccines are developed to safeguard...

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Autores principales: Lokhandwala, Shehnaz, Waghela, Suryakant D., Bray, Jocelyn, Sangewar, Neha, Charendoff, Chloe, Martin, Cameron L., Hassan, Wisam S., Koynarski, Tsvetoslav, Gabbert, Lindsay, Burrage, Thomas G., Brake, David, Neilan, John, Mwangi, Waithaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5421782/
https://www.ncbi.nlm.nih.gov/pubmed/28481911
http://dx.doi.org/10.1371/journal.pone.0177007
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author Lokhandwala, Shehnaz
Waghela, Suryakant D.
Bray, Jocelyn
Sangewar, Neha
Charendoff, Chloe
Martin, Cameron L.
Hassan, Wisam S.
Koynarski, Tsvetoslav
Gabbert, Lindsay
Burrage, Thomas G.
Brake, David
Neilan, John
Mwangi, Waithaka
author_facet Lokhandwala, Shehnaz
Waghela, Suryakant D.
Bray, Jocelyn
Sangewar, Neha
Charendoff, Chloe
Martin, Cameron L.
Hassan, Wisam S.
Koynarski, Tsvetoslav
Gabbert, Lindsay
Burrage, Thomas G.
Brake, David
Neilan, John
Mwangi, Waithaka
author_sort Lokhandwala, Shehnaz
collection PubMed
description African Swine Fever Virus (ASFV) is a high-consequence transboundary animal pathogen that often causes hemorrhagic disease in swine with a case fatality rate close to 100%. Lack of treatment or vaccine for the disease makes it imperative that safe and efficacious vaccines are developed to safeguard the swine industry. In this study, we evaluated the immunogenicity of seven adenovirus-vectored novel ASFV antigens, namely A151R, B119L, B602L, EP402RΔPRR, B438L, K205R and A104R. Immunization of commercial swine with a cocktail of the recombinant adenoviruses formulated in adjuvant primed strong ASFV antigen-specific IgG responses that underwent rapid recall upon boost. Notably, most vaccinees mounted robust IgG responses against all the antigens in the cocktail. Most importantly and relevant to vaccine development, the induced antibodies recognized viral proteins from Georgia 2007/1 ASFV-infected cells by IFA and by western blot analysis. The recombinant adenovirus cocktail also induced ASFV-specific IFN-γ-secreting cells that were recalled upon boosting. Evaluation of local and systemic effects of the recombinant adenovirus cocktail post-priming and post-boosting in the immunized animals showed that the immunogen was well tolerated and no serious negative effects were observed. Taken together, these outcomes showed that the adenovirus-vectored novel ASFV antigen cocktail was capable of safely inducing strong antibody and IFN-γ(+) cell responses in commercial swine. The data will be used for selection of antigens for inclusion in a multi-antigen prototype vaccine to be evaluated for protective efficacy.
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spelling pubmed-54217822017-05-14 Adenovirus-vectored novel African Swine Fever Virus antigens elicit robust immune responses in swine Lokhandwala, Shehnaz Waghela, Suryakant D. Bray, Jocelyn Sangewar, Neha Charendoff, Chloe Martin, Cameron L. Hassan, Wisam S. Koynarski, Tsvetoslav Gabbert, Lindsay Burrage, Thomas G. Brake, David Neilan, John Mwangi, Waithaka PLoS One Research Article African Swine Fever Virus (ASFV) is a high-consequence transboundary animal pathogen that often causes hemorrhagic disease in swine with a case fatality rate close to 100%. Lack of treatment or vaccine for the disease makes it imperative that safe and efficacious vaccines are developed to safeguard the swine industry. In this study, we evaluated the immunogenicity of seven adenovirus-vectored novel ASFV antigens, namely A151R, B119L, B602L, EP402RΔPRR, B438L, K205R and A104R. Immunization of commercial swine with a cocktail of the recombinant adenoviruses formulated in adjuvant primed strong ASFV antigen-specific IgG responses that underwent rapid recall upon boost. Notably, most vaccinees mounted robust IgG responses against all the antigens in the cocktail. Most importantly and relevant to vaccine development, the induced antibodies recognized viral proteins from Georgia 2007/1 ASFV-infected cells by IFA and by western blot analysis. The recombinant adenovirus cocktail also induced ASFV-specific IFN-γ-secreting cells that were recalled upon boosting. Evaluation of local and systemic effects of the recombinant adenovirus cocktail post-priming and post-boosting in the immunized animals showed that the immunogen was well tolerated and no serious negative effects were observed. Taken together, these outcomes showed that the adenovirus-vectored novel ASFV antigen cocktail was capable of safely inducing strong antibody and IFN-γ(+) cell responses in commercial swine. The data will be used for selection of antigens for inclusion in a multi-antigen prototype vaccine to be evaluated for protective efficacy. Public Library of Science 2017-05-08 /pmc/articles/PMC5421782/ /pubmed/28481911 http://dx.doi.org/10.1371/journal.pone.0177007 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Lokhandwala, Shehnaz
Waghela, Suryakant D.
Bray, Jocelyn
Sangewar, Neha
Charendoff, Chloe
Martin, Cameron L.
Hassan, Wisam S.
Koynarski, Tsvetoslav
Gabbert, Lindsay
Burrage, Thomas G.
Brake, David
Neilan, John
Mwangi, Waithaka
Adenovirus-vectored novel African Swine Fever Virus antigens elicit robust immune responses in swine
title Adenovirus-vectored novel African Swine Fever Virus antigens elicit robust immune responses in swine
title_full Adenovirus-vectored novel African Swine Fever Virus antigens elicit robust immune responses in swine
title_fullStr Adenovirus-vectored novel African Swine Fever Virus antigens elicit robust immune responses in swine
title_full_unstemmed Adenovirus-vectored novel African Swine Fever Virus antigens elicit robust immune responses in swine
title_short Adenovirus-vectored novel African Swine Fever Virus antigens elicit robust immune responses in swine
title_sort adenovirus-vectored novel african swine fever virus antigens elicit robust immune responses in swine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5421782/
https://www.ncbi.nlm.nih.gov/pubmed/28481911
http://dx.doi.org/10.1371/journal.pone.0177007
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