Cargando…
MiR-675-5p supports hypoxia induced epithelial to mesenchymal transition in colon cancer cells
The survival rates in colon cancer patients are inversely proportional to the number of lymph node metastases. The hypoxia-induced Epithelial to Mesenchymal Transition (EMT), driven by HIF1α, is known to be involved in cancer progression and metastasis. Recently, we have reported that miR-675-5p pro...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5421847/ https://www.ncbi.nlm.nih.gov/pubmed/28061476 http://dx.doi.org/10.18632/oncotarget.14464 |
_version_ | 1783234662637764608 |
---|---|
author | Costa, Viviana Dico, Alessia Lo Rizzo, Aroldo Rajata, Francesca Tripodi, Marco Alessand, Riccardo Conigliaro, Alice |
author_facet | Costa, Viviana Dico, Alessia Lo Rizzo, Aroldo Rajata, Francesca Tripodi, Marco Alessand, Riccardo Conigliaro, Alice |
author_sort | Costa, Viviana |
collection | PubMed |
description | The survival rates in colon cancer patients are inversely proportional to the number of lymph node metastases. The hypoxia-induced Epithelial to Mesenchymal Transition (EMT), driven by HIF1α, is known to be involved in cancer progression and metastasis. Recently, we have reported that miR-675-5p promotes glioma growth by stabilizing HIF1α; here, by use of the syngeneic cell lines we investigated the role of the miR-675-5p in colon cancer metastasis. Our results show that miR-675-5p, over expressed in metastatic colon cancer cells, participates to tumour progression by regulating HIF1α induced EMT. MiR-675-5p increases Snail transcription by a dual strategy: i) stabilizing the activity of the transcription factor HIF1α and ii) and inhibiting Snail's repressor DDB2 (Damage specific DNA Binding protein 2). Moreover, transcriptional analyses on specimens from colon cancer patients confirmed, in vivo, the correlation between miR-675-5p over-expression and metastasis, thus identifying miR-675-5p as a new marker for colon cancer progression and therefore a putative target for therapeutic strategies. |
format | Online Article Text |
id | pubmed-5421847 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-54218472017-05-10 MiR-675-5p supports hypoxia induced epithelial to mesenchymal transition in colon cancer cells Costa, Viviana Dico, Alessia Lo Rizzo, Aroldo Rajata, Francesca Tripodi, Marco Alessand, Riccardo Conigliaro, Alice Oncotarget Research Paper The survival rates in colon cancer patients are inversely proportional to the number of lymph node metastases. The hypoxia-induced Epithelial to Mesenchymal Transition (EMT), driven by HIF1α, is known to be involved in cancer progression and metastasis. Recently, we have reported that miR-675-5p promotes glioma growth by stabilizing HIF1α; here, by use of the syngeneic cell lines we investigated the role of the miR-675-5p in colon cancer metastasis. Our results show that miR-675-5p, over expressed in metastatic colon cancer cells, participates to tumour progression by regulating HIF1α induced EMT. MiR-675-5p increases Snail transcription by a dual strategy: i) stabilizing the activity of the transcription factor HIF1α and ii) and inhibiting Snail's repressor DDB2 (Damage specific DNA Binding protein 2). Moreover, transcriptional analyses on specimens from colon cancer patients confirmed, in vivo, the correlation between miR-675-5p over-expression and metastasis, thus identifying miR-675-5p as a new marker for colon cancer progression and therefore a putative target for therapeutic strategies. Impact Journals LLC 2017-01-03 /pmc/articles/PMC5421847/ /pubmed/28061476 http://dx.doi.org/10.18632/oncotarget.14464 Text en Copyright: © 2017 Costa et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Costa, Viviana Dico, Alessia Lo Rizzo, Aroldo Rajata, Francesca Tripodi, Marco Alessand, Riccardo Conigliaro, Alice MiR-675-5p supports hypoxia induced epithelial to mesenchymal transition in colon cancer cells |
title | MiR-675-5p supports hypoxia induced epithelial to mesenchymal transition in colon cancer cells |
title_full | MiR-675-5p supports hypoxia induced epithelial to mesenchymal transition in colon cancer cells |
title_fullStr | MiR-675-5p supports hypoxia induced epithelial to mesenchymal transition in colon cancer cells |
title_full_unstemmed | MiR-675-5p supports hypoxia induced epithelial to mesenchymal transition in colon cancer cells |
title_short | MiR-675-5p supports hypoxia induced epithelial to mesenchymal transition in colon cancer cells |
title_sort | mir-675-5p supports hypoxia induced epithelial to mesenchymal transition in colon cancer cells |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5421847/ https://www.ncbi.nlm.nih.gov/pubmed/28061476 http://dx.doi.org/10.18632/oncotarget.14464 |
work_keys_str_mv | AT costaviviana mir6755psupportshypoxiainducedepithelialtomesenchymaltransitionincoloncancercells AT dicoalessialo mir6755psupportshypoxiainducedepithelialtomesenchymaltransitionincoloncancercells AT rizzoaroldo mir6755psupportshypoxiainducedepithelialtomesenchymaltransitionincoloncancercells AT rajatafrancesca mir6755psupportshypoxiainducedepithelialtomesenchymaltransitionincoloncancercells AT tripodimarco mir6755psupportshypoxiainducedepithelialtomesenchymaltransitionincoloncancercells AT alessandriccardo mir6755psupportshypoxiainducedepithelialtomesenchymaltransitionincoloncancercells AT conigliaroalice mir6755psupportshypoxiainducedepithelialtomesenchymaltransitionincoloncancercells |