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Miniaturized Real-Time PCR on a Q3 System for Rapid KRAS Genotyping
Colorectal cancer (CRC) is an aggressive human malignancy with a complex genomic landscape harboring KRAS mutations. In 40%–60% of patients with CRC, constantly active KRAS proteins affect the prognosis, surgical strategy, and clinical benefit from therapy with anti-epidermal growth factor receptor...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5422192/ https://www.ncbi.nlm.nih.gov/pubmed/28398227 http://dx.doi.org/10.3390/s17040831 |
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author | Guarnaccia, Maria Iemmolo, Rosario Petralia, Salvatore Conoci, Sabrina Cavallaro, Sebastiano |
author_facet | Guarnaccia, Maria Iemmolo, Rosario Petralia, Salvatore Conoci, Sabrina Cavallaro, Sebastiano |
author_sort | Guarnaccia, Maria |
collection | PubMed |
description | Colorectal cancer (CRC) is an aggressive human malignancy with a complex genomic landscape harboring KRAS mutations. In 40%–60% of patients with CRC, constantly active KRAS proteins affect the prognosis, surgical strategy, and clinical benefit from therapy with anti-epidermal growth factor receptor (EGFR) agents. For this reason, there is a greater demand for minimally-invasive diagnostic devices to characterize the genetic pattern and prevent the acquired mechanism to drug resistance. The rapid developments in cutting-edge diagnostic techniques are expected to play a growing role in medicine and represent an attractive promise to identify potential responders to personalized medicine. Here we propose a new method to simultaneously detect the main KRAS mutations on the portable real-time PCR Q3 platform. This platform is based on hybrid silicon-plastic technology implemented in a miniaturized chip able to achieve a sample-in answer-out rapid analysis, allowing a new approach to genetic counseling and testing. |
format | Online Article Text |
id | pubmed-5422192 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54221922017-05-12 Miniaturized Real-Time PCR on a Q3 System for Rapid KRAS Genotyping Guarnaccia, Maria Iemmolo, Rosario Petralia, Salvatore Conoci, Sabrina Cavallaro, Sebastiano Sensors (Basel) Article Colorectal cancer (CRC) is an aggressive human malignancy with a complex genomic landscape harboring KRAS mutations. In 40%–60% of patients with CRC, constantly active KRAS proteins affect the prognosis, surgical strategy, and clinical benefit from therapy with anti-epidermal growth factor receptor (EGFR) agents. For this reason, there is a greater demand for minimally-invasive diagnostic devices to characterize the genetic pattern and prevent the acquired mechanism to drug resistance. The rapid developments in cutting-edge diagnostic techniques are expected to play a growing role in medicine and represent an attractive promise to identify potential responders to personalized medicine. Here we propose a new method to simultaneously detect the main KRAS mutations on the portable real-time PCR Q3 platform. This platform is based on hybrid silicon-plastic technology implemented in a miniaturized chip able to achieve a sample-in answer-out rapid analysis, allowing a new approach to genetic counseling and testing. MDPI 2017-04-11 /pmc/articles/PMC5422192/ /pubmed/28398227 http://dx.doi.org/10.3390/s17040831 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Guarnaccia, Maria Iemmolo, Rosario Petralia, Salvatore Conoci, Sabrina Cavallaro, Sebastiano Miniaturized Real-Time PCR on a Q3 System for Rapid KRAS Genotyping |
title | Miniaturized Real-Time PCR on a Q3 System for Rapid KRAS Genotyping |
title_full | Miniaturized Real-Time PCR on a Q3 System for Rapid KRAS Genotyping |
title_fullStr | Miniaturized Real-Time PCR on a Q3 System for Rapid KRAS Genotyping |
title_full_unstemmed | Miniaturized Real-Time PCR on a Q3 System for Rapid KRAS Genotyping |
title_short | Miniaturized Real-Time PCR on a Q3 System for Rapid KRAS Genotyping |
title_sort | miniaturized real-time pcr on a q3 system for rapid kras genotyping |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5422192/ https://www.ncbi.nlm.nih.gov/pubmed/28398227 http://dx.doi.org/10.3390/s17040831 |
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