In Vitro Comparison of Biological Effects of Coe-Pak and Reso-Pac Periodontal Dressings

OBJECTIVES: The purpose of the present study was to compare the cytotoxicity of Reso-Pac and Coe-Pak periodontal dressing. MATERIAL AND METHODS: According to ISO-10993-12:2012, 1-, 3- and 7-day extracts of the two periodontal dressings were prepared in cell culture medium and exposed to the two cultured cell lines. Cell viability and proliferation at 24 h and 72 h following exposure were evaluated using quantitative MTT assay. RESULTS: The results showed a significant (P < 0.05) reduction in the viability of cells exposed to the 3- and 7-day Coe-Pak extracts at 24 h and 72 h compared to the control group (no exposure to the extract). Reso-Pac extracts slightly decreased cell viability compared to the control group. Understudy materials showed greater cytotoxicity against human osteoblast-like compared to the human gingival fibroblast cells. No significant (P > 0.05) difference was found in the viability of cells exposed to undiluted (100%) one-day extract and diluted (50%) extract of both understudy materials at 24 h and 72 h after exposure. CONCLUSIONS: Based on the results, Reso-Pac periodontal dressing has less cytotoxicity than Coe-Pak.