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PARV4 prevalence, phylogeny, immunology and coinfection with HIV, HBV and HCV in a multicentre African cohort
Background: The seroprevalence of human parvovirus-4 (PARV4) varies considerably by region. In sub-Saharan Africa, seroprevalence is high in the general population, but little is known about the transmission routes or the prevalence of coinfection with blood-borne viruses, HBV, HCV and HIV. Methods...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
F1000Research
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5423528/ https://www.ncbi.nlm.nih.gov/pubmed/28497124 http://dx.doi.org/10.12688/wellcomeopenres.11135.1 |
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author | Sharp, Colin P. Gregory, William F. Hattingh, Louise Malik, Amna Adland, Emily Daniels, Samantha van Zyl, Anriette Carlson, Jonathan M. Wareing, Susan Ogwu, Anthony Shapiro, Roger Riddell, Lynn Chen, Fabian Ndung'u, Thumbi Goulder, Philip J.R. Klenerman, Paul Simmonds, Peter Jooste, Pieter Matthews, Philippa C. |
author_facet | Sharp, Colin P. Gregory, William F. Hattingh, Louise Malik, Amna Adland, Emily Daniels, Samantha van Zyl, Anriette Carlson, Jonathan M. Wareing, Susan Ogwu, Anthony Shapiro, Roger Riddell, Lynn Chen, Fabian Ndung'u, Thumbi Goulder, Philip J.R. Klenerman, Paul Simmonds, Peter Jooste, Pieter Matthews, Philippa C. |
author_sort | Sharp, Colin P. |
collection | PubMed |
description | Background: The seroprevalence of human parvovirus-4 (PARV4) varies considerably by region. In sub-Saharan Africa, seroprevalence is high in the general population, but little is known about the transmission routes or the prevalence of coinfection with blood-borne viruses, HBV, HCV and HIV. Methods: To further explore the characteristics of PARV4 in this setting, with a particular focus on the prevalence and significance of coinfection, we screened a cohort of 695 individuals recruited from Durban and Kimberley (South Africa) and Gaborone (Botswana) for PARV4 IgG and DNA, as well as documenting HIV, HBV and HCV status. Results: Within these cohorts, 69% of subjects were HIV-positive. We identified no cases of HCV by PCR, but 7.4% were positive for HBsAg. PARV4 IgG was positive in 42%; seroprevalence was higher in adults (69%) compared to children (21%) (p<0.0001) and in HIV-positive (52%) compared to HIV-negative individuals (24%) (p<0.0001), but there was no association with HBsAg status. We developed an on-line tool to allow visualization of coinfection data ( https://purl.oclc.org/coinfection-viz). We identified five subjects who were PCR-positive for PARV4 genotype-3. Ex vivo CD8+ T cell responses spanned the entire PARV4 proteome and we propose a novel HLA-B*57:03-restricted epitope within the NS protein. Conclusions: This characterisation of PARV4 infection provides enhanced insights into the epidemiology of infection and co-infection in African cohorts, and provides the foundations for planning further focused studies to elucidate transmission pathways, immune responses, and the clinical significance of this organism. |
format | Online Article Text |
id | pubmed-5423528 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | F1000Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-54235282017-05-09 PARV4 prevalence, phylogeny, immunology and coinfection with HIV, HBV and HCV in a multicentre African cohort Sharp, Colin P. Gregory, William F. Hattingh, Louise Malik, Amna Adland, Emily Daniels, Samantha van Zyl, Anriette Carlson, Jonathan M. Wareing, Susan Ogwu, Anthony Shapiro, Roger Riddell, Lynn Chen, Fabian Ndung'u, Thumbi Goulder, Philip J.R. Klenerman, Paul Simmonds, Peter Jooste, Pieter Matthews, Philippa C. Wellcome Open Res Research Article Background: The seroprevalence of human parvovirus-4 (PARV4) varies considerably by region. In sub-Saharan Africa, seroprevalence is high in the general population, but little is known about the transmission routes or the prevalence of coinfection with blood-borne viruses, HBV, HCV and HIV. Methods: To further explore the characteristics of PARV4 in this setting, with a particular focus on the prevalence and significance of coinfection, we screened a cohort of 695 individuals recruited from Durban and Kimberley (South Africa) and Gaborone (Botswana) for PARV4 IgG and DNA, as well as documenting HIV, HBV and HCV status. Results: Within these cohorts, 69% of subjects were HIV-positive. We identified no cases of HCV by PCR, but 7.4% were positive for HBsAg. PARV4 IgG was positive in 42%; seroprevalence was higher in adults (69%) compared to children (21%) (p<0.0001) and in HIV-positive (52%) compared to HIV-negative individuals (24%) (p<0.0001), but there was no association with HBsAg status. We developed an on-line tool to allow visualization of coinfection data ( https://purl.oclc.org/coinfection-viz). We identified five subjects who were PCR-positive for PARV4 genotype-3. Ex vivo CD8+ T cell responses spanned the entire PARV4 proteome and we propose a novel HLA-B*57:03-restricted epitope within the NS protein. Conclusions: This characterisation of PARV4 infection provides enhanced insights into the epidemiology of infection and co-infection in African cohorts, and provides the foundations for planning further focused studies to elucidate transmission pathways, immune responses, and the clinical significance of this organism. F1000Research 2017-04-07 /pmc/articles/PMC5423528/ /pubmed/28497124 http://dx.doi.org/10.12688/wellcomeopenres.11135.1 Text en Copyright: © 2017 Sharp CP et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Sharp, Colin P. Gregory, William F. Hattingh, Louise Malik, Amna Adland, Emily Daniels, Samantha van Zyl, Anriette Carlson, Jonathan M. Wareing, Susan Ogwu, Anthony Shapiro, Roger Riddell, Lynn Chen, Fabian Ndung'u, Thumbi Goulder, Philip J.R. Klenerman, Paul Simmonds, Peter Jooste, Pieter Matthews, Philippa C. PARV4 prevalence, phylogeny, immunology and coinfection with HIV, HBV and HCV in a multicentre African cohort |
title | PARV4 prevalence, phylogeny, immunology and coinfection with HIV, HBV and HCV in a multicentre African cohort |
title_full | PARV4 prevalence, phylogeny, immunology and coinfection with HIV, HBV and HCV in a multicentre African cohort |
title_fullStr | PARV4 prevalence, phylogeny, immunology and coinfection with HIV, HBV and HCV in a multicentre African cohort |
title_full_unstemmed | PARV4 prevalence, phylogeny, immunology and coinfection with HIV, HBV and HCV in a multicentre African cohort |
title_short | PARV4 prevalence, phylogeny, immunology and coinfection with HIV, HBV and HCV in a multicentre African cohort |
title_sort | parv4 prevalence, phylogeny, immunology and coinfection with hiv, hbv and hcv in a multicentre african cohort |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5423528/ https://www.ncbi.nlm.nih.gov/pubmed/28497124 http://dx.doi.org/10.12688/wellcomeopenres.11135.1 |
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