Cargando…
Antibiofilm and antibacterial effects of specific chitosan molecules on Staphylococcus aureus isolates associated with bovine mastitis
Staphylococcus aureus is one of the major pathogens causing bovine intramammary infections (IMIs) and mastitis. Mastitis is the primary cause for the use of antibiotics in dairy farms but therapeutic failure is often observed. One of the reasons for the lack of effectiveness of antibiotic therapy de...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5423679/ https://www.ncbi.nlm.nih.gov/pubmed/28486482 http://dx.doi.org/10.1371/journal.pone.0176988 |
_version_ | 1783234992361439232 |
---|---|
author | Asli, Abdelhamid Brouillette, Eric Ster, Céline Ghinet, Mariana Gabriela Brzezinski, Ryszard Lacasse, Pierre Jacques, Mario Malouin, François |
author_facet | Asli, Abdelhamid Brouillette, Eric Ster, Céline Ghinet, Mariana Gabriela Brzezinski, Ryszard Lacasse, Pierre Jacques, Mario Malouin, François |
author_sort | Asli, Abdelhamid |
collection | PubMed |
description | Staphylococcus aureus is one of the major pathogens causing bovine intramammary infections (IMIs) and mastitis. Mastitis is the primary cause for the use of antibiotics in dairy farms but therapeutic failure is often observed. One of the reasons for the lack of effectiveness of antibiotic therapy despite the observed susceptibility of bacterial isolates in vitro are bacterial biofilms. In this study, we used chitosan of well-defined molecular weight (0.4–0.6, 1.3, 2.6 and 4.0 kDa) and investigated their antibiofilm and antibacterial activities in in vitro and in vivo models related to S. aureus IMIs. A chitosan of at least 6 units of glucosamine was necessary for maximum antibacterial activity. The 2.6 and 4.0 kDa forms were able to prevent biofilm production by the biofilm hyperproducer strain S. aureus 2117 and a bovine MRSA (methicillin-resistant S. aureus). The intramammary administration of the 2.6 kDa chitosan showed no adverse effects in mice or in cows, as opposed to the slight inflammatory effect observed in mammary glands with the 4.0 kDa derivative. The 2.6 kDa chitosan killed bacteria embedded in pre-established biofilms in a dose-dependent manner with a >3 log10 reduction in CFU at 4 mg/ml. Also, the 2.6 kDa chitosan could prevent the persistence of the internalized MRSA into the mammary epithelial cell line MAC-T. An in vitro checkerboard assay showed that the 2.6 kDa chitosan produced a synergy with the macrolide class of antibiotics (e.g., tilmicosin) and reduced the MIC of both molecules by 2–8 times. Finally, the intramammary administration of the 2.6 kDa chitosan alone (P<0.01) or in combination with tilmicosin (P<0.0001) reduced the colonization of mammary glands in a murine IMI model. Our results suggest that the use of chitosan alone or in combination with a low dose of a macrolide could help reduce antibiotic use in dairy farms. |
format | Online Article Text |
id | pubmed-5423679 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54236792017-05-15 Antibiofilm and antibacterial effects of specific chitosan molecules on Staphylococcus aureus isolates associated with bovine mastitis Asli, Abdelhamid Brouillette, Eric Ster, Céline Ghinet, Mariana Gabriela Brzezinski, Ryszard Lacasse, Pierre Jacques, Mario Malouin, François PLoS One Research Article Staphylococcus aureus is one of the major pathogens causing bovine intramammary infections (IMIs) and mastitis. Mastitis is the primary cause for the use of antibiotics in dairy farms but therapeutic failure is often observed. One of the reasons for the lack of effectiveness of antibiotic therapy despite the observed susceptibility of bacterial isolates in vitro are bacterial biofilms. In this study, we used chitosan of well-defined molecular weight (0.4–0.6, 1.3, 2.6 and 4.0 kDa) and investigated their antibiofilm and antibacterial activities in in vitro and in vivo models related to S. aureus IMIs. A chitosan of at least 6 units of glucosamine was necessary for maximum antibacterial activity. The 2.6 and 4.0 kDa forms were able to prevent biofilm production by the biofilm hyperproducer strain S. aureus 2117 and a bovine MRSA (methicillin-resistant S. aureus). The intramammary administration of the 2.6 kDa chitosan showed no adverse effects in mice or in cows, as opposed to the slight inflammatory effect observed in mammary glands with the 4.0 kDa derivative. The 2.6 kDa chitosan killed bacteria embedded in pre-established biofilms in a dose-dependent manner with a >3 log10 reduction in CFU at 4 mg/ml. Also, the 2.6 kDa chitosan could prevent the persistence of the internalized MRSA into the mammary epithelial cell line MAC-T. An in vitro checkerboard assay showed that the 2.6 kDa chitosan produced a synergy with the macrolide class of antibiotics (e.g., tilmicosin) and reduced the MIC of both molecules by 2–8 times. Finally, the intramammary administration of the 2.6 kDa chitosan alone (P<0.01) or in combination with tilmicosin (P<0.0001) reduced the colonization of mammary glands in a murine IMI model. Our results suggest that the use of chitosan alone or in combination with a low dose of a macrolide could help reduce antibiotic use in dairy farms. Public Library of Science 2017-05-09 /pmc/articles/PMC5423679/ /pubmed/28486482 http://dx.doi.org/10.1371/journal.pone.0176988 Text en © 2017 Asli et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Asli, Abdelhamid Brouillette, Eric Ster, Céline Ghinet, Mariana Gabriela Brzezinski, Ryszard Lacasse, Pierre Jacques, Mario Malouin, François Antibiofilm and antibacterial effects of specific chitosan molecules on Staphylococcus aureus isolates associated with bovine mastitis |
title | Antibiofilm and antibacterial effects of specific chitosan molecules on Staphylococcus aureus isolates associated with bovine mastitis |
title_full | Antibiofilm and antibacterial effects of specific chitosan molecules on Staphylococcus aureus isolates associated with bovine mastitis |
title_fullStr | Antibiofilm and antibacterial effects of specific chitosan molecules on Staphylococcus aureus isolates associated with bovine mastitis |
title_full_unstemmed | Antibiofilm and antibacterial effects of specific chitosan molecules on Staphylococcus aureus isolates associated with bovine mastitis |
title_short | Antibiofilm and antibacterial effects of specific chitosan molecules on Staphylococcus aureus isolates associated with bovine mastitis |
title_sort | antibiofilm and antibacterial effects of specific chitosan molecules on staphylococcus aureus isolates associated with bovine mastitis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5423679/ https://www.ncbi.nlm.nih.gov/pubmed/28486482 http://dx.doi.org/10.1371/journal.pone.0176988 |
work_keys_str_mv | AT asliabdelhamid antibiofilmandantibacterialeffectsofspecificchitosanmoleculesonstaphylococcusaureusisolatesassociatedwithbovinemastitis AT brouilletteeric antibiofilmandantibacterialeffectsofspecificchitosanmoleculesonstaphylococcusaureusisolatesassociatedwithbovinemastitis AT sterceline antibiofilmandantibacterialeffectsofspecificchitosanmoleculesonstaphylococcusaureusisolatesassociatedwithbovinemastitis AT ghinetmarianagabriela antibiofilmandantibacterialeffectsofspecificchitosanmoleculesonstaphylococcusaureusisolatesassociatedwithbovinemastitis AT brzezinskiryszard antibiofilmandantibacterialeffectsofspecificchitosanmoleculesonstaphylococcusaureusisolatesassociatedwithbovinemastitis AT lacassepierre antibiofilmandantibacterialeffectsofspecificchitosanmoleculesonstaphylococcusaureusisolatesassociatedwithbovinemastitis AT jacquesmario antibiofilmandantibacterialeffectsofspecificchitosanmoleculesonstaphylococcusaureusisolatesassociatedwithbovinemastitis AT malouinfrancois antibiofilmandantibacterialeffectsofspecificchitosanmoleculesonstaphylococcusaureusisolatesassociatedwithbovinemastitis |