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Novel Mechanisms Revealed in the Trachea Transcriptome of Resistant and Susceptible Chicken Lines following Infection with Newcastle Disease Virus

Newcastle disease virus (NDV) has a devastating impact on poultry production in developing countries. This study examined the transcriptome of tracheal epithelial cells from two inbred chicken lines that differ in NDV susceptibility after challenge with a high-titer inoculum of lentogenic NDV. The F...

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Autores principales: Deist, Melissa S., Gallardo, Rodrigo A., Bunn, David A., Kelly, Terra R., Dekkers, Jack C. M., Zhou, Huaijun, Lamont, Susan J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5424241/
https://www.ncbi.nlm.nih.gov/pubmed/28331077
http://dx.doi.org/10.1128/CVI.00027-17
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author Deist, Melissa S.
Gallardo, Rodrigo A.
Bunn, David A.
Kelly, Terra R.
Dekkers, Jack C. M.
Zhou, Huaijun
Lamont, Susan J.
author_facet Deist, Melissa S.
Gallardo, Rodrigo A.
Bunn, David A.
Kelly, Terra R.
Dekkers, Jack C. M.
Zhou, Huaijun
Lamont, Susan J.
author_sort Deist, Melissa S.
collection PubMed
description Newcastle disease virus (NDV) has a devastating impact on poultry production in developing countries. This study examined the transcriptome of tracheal epithelial cells from two inbred chicken lines that differ in NDV susceptibility after challenge with a high-titer inoculum of lentogenic NDV. The Fayoumi line had a significantly lower NDV load postchallenge than the Leghorn line, demonstrating the Fayoumi line's classification as a relatively NDV-resistant breed. Examination of the trachea transcriptome showed a large increase in immune cell infiltration in the trachea in both lines at all times postinfection. The pathways conserved across lines and at all three time points postinfection included iCOS-iCOSL signaling in T helper cells, NF-κB signaling, the role of nuclear factor of activated T cells in the regulation of the immune response, calcium-induced T lymphocyte apoptosis, phospholipase C signaling, and CD28 signaling in T helper cells. Although shared pathways were seen in the Fayoumi and Leghorn lines, each line showed unique responses as well. The downregulation of collagen and the activation of eukaryotic translation initiation factor 2 signaling in the Fayoumis relative to the Leghorns at 2 days postinfection may contribute to the resistance phenotype seen in the Fayoumis. This study provides a further understanding of host-pathogen interactions which could improve vaccine efficacy and, in combination with genome-wide association studies, has the potential to advance strategies for breeding chickens with enhanced resistance to NDV.
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spelling pubmed-54242412017-05-19 Novel Mechanisms Revealed in the Trachea Transcriptome of Resistant and Susceptible Chicken Lines following Infection with Newcastle Disease Virus Deist, Melissa S. Gallardo, Rodrigo A. Bunn, David A. Kelly, Terra R. Dekkers, Jack C. M. Zhou, Huaijun Lamont, Susan J. Clin Vaccine Immunol Clinical Immunology Newcastle disease virus (NDV) has a devastating impact on poultry production in developing countries. This study examined the transcriptome of tracheal epithelial cells from two inbred chicken lines that differ in NDV susceptibility after challenge with a high-titer inoculum of lentogenic NDV. The Fayoumi line had a significantly lower NDV load postchallenge than the Leghorn line, demonstrating the Fayoumi line's classification as a relatively NDV-resistant breed. Examination of the trachea transcriptome showed a large increase in immune cell infiltration in the trachea in both lines at all times postinfection. The pathways conserved across lines and at all three time points postinfection included iCOS-iCOSL signaling in T helper cells, NF-κB signaling, the role of nuclear factor of activated T cells in the regulation of the immune response, calcium-induced T lymphocyte apoptosis, phospholipase C signaling, and CD28 signaling in T helper cells. Although shared pathways were seen in the Fayoumi and Leghorn lines, each line showed unique responses as well. The downregulation of collagen and the activation of eukaryotic translation initiation factor 2 signaling in the Fayoumis relative to the Leghorns at 2 days postinfection may contribute to the resistance phenotype seen in the Fayoumis. This study provides a further understanding of host-pathogen interactions which could improve vaccine efficacy and, in combination with genome-wide association studies, has the potential to advance strategies for breeding chickens with enhanced resistance to NDV. American Society for Microbiology 2017-05-05 /pmc/articles/PMC5424241/ /pubmed/28331077 http://dx.doi.org/10.1128/CVI.00027-17 Text en Copyright © 2017 Deist et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Clinical Immunology
Deist, Melissa S.
Gallardo, Rodrigo A.
Bunn, David A.
Kelly, Terra R.
Dekkers, Jack C. M.
Zhou, Huaijun
Lamont, Susan J.
Novel Mechanisms Revealed in the Trachea Transcriptome of Resistant and Susceptible Chicken Lines following Infection with Newcastle Disease Virus
title Novel Mechanisms Revealed in the Trachea Transcriptome of Resistant and Susceptible Chicken Lines following Infection with Newcastle Disease Virus
title_full Novel Mechanisms Revealed in the Trachea Transcriptome of Resistant and Susceptible Chicken Lines following Infection with Newcastle Disease Virus
title_fullStr Novel Mechanisms Revealed in the Trachea Transcriptome of Resistant and Susceptible Chicken Lines following Infection with Newcastle Disease Virus
title_full_unstemmed Novel Mechanisms Revealed in the Trachea Transcriptome of Resistant and Susceptible Chicken Lines following Infection with Newcastle Disease Virus
title_short Novel Mechanisms Revealed in the Trachea Transcriptome of Resistant and Susceptible Chicken Lines following Infection with Newcastle Disease Virus
title_sort novel mechanisms revealed in the trachea transcriptome of resistant and susceptible chicken lines following infection with newcastle disease virus
topic Clinical Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5424241/
https://www.ncbi.nlm.nih.gov/pubmed/28331077
http://dx.doi.org/10.1128/CVI.00027-17
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