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Zanthoxylum alkylamides ameliorate protein metabolism disorder in STZ-induced diabetic rats

This study aimed to evaluate the protein metabolism effect of Zanthoxylum alkylamides and to explore the potential mechanism in streptozotocin (STZ)-induced diabetic rats. Diabetic rats were orally treated with 2, 4 and 8 mg per kg bw of alkylamides daily for 28 days. Alkylamides decreased the relat...

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Autores principales: Ren, Tingyuan, Zhu, Yuping, Xia, Xuejuan, Ding, Yongbo, Guo, Jing, Kan, Jianquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bioscientifica Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5424265/
https://www.ncbi.nlm.nih.gov/pubmed/28100702
http://dx.doi.org/10.1530/JME-16-0218
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author Ren, Tingyuan
Zhu, Yuping
Xia, Xuejuan
Ding, Yongbo
Guo, Jing
Kan, Jianquan
author_facet Ren, Tingyuan
Zhu, Yuping
Xia, Xuejuan
Ding, Yongbo
Guo, Jing
Kan, Jianquan
author_sort Ren, Tingyuan
collection PubMed
description This study aimed to evaluate the protein metabolism effect of Zanthoxylum alkylamides and to explore the potential mechanism in streptozotocin (STZ)-induced diabetic rats. Diabetic rats were orally treated with 2, 4 and 8 mg per kg bw of alkylamides daily for 28 days. Alkylamides decreased the relative weight of the liver and food intake, significantly increased the relative skeletal muscle weight and significantly decreased the blood urea nitrogen levels. Insulin, insulin-like growth factor 1, total protein (TP) and albumin (ALB), globular proteins and ALB proteins/globulin protein levels in serum significantly increased. TP, RNA content and RNA/DNA ratio significantly increased in the skeletal muscle of diabetic rats. Real-time quantitative polymerase chain reaction results indicated that alkylamides significantly increased the mRNA expression of insulin receptor (InR), IGF1 and insulin-like growth factor 1 receptor (IGF1R) in the liver and skeletal muscle. Moreover, the mRNA and protein expression levels of PI3K, PKB and mTOR significantly increased, whereas those of atrogin-1, muscle ring finger 1 and FOXO in the skeletal muscle significantly decreased. Alkylamides may advance protein synthesis by the PI3K/PKB/mTOR signalling pathway and attenuate the catabolism of protein through the ubiquitin–proteasome pathway. Therefore, it was possible that alkylamides ameliorate protein metabolism disorders in diabetic rats by activating the mTOR pathway.
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spelling pubmed-54242652017-05-11 Zanthoxylum alkylamides ameliorate protein metabolism disorder in STZ-induced diabetic rats Ren, Tingyuan Zhu, Yuping Xia, Xuejuan Ding, Yongbo Guo, Jing Kan, Jianquan J Mol Endocrinol Research This study aimed to evaluate the protein metabolism effect of Zanthoxylum alkylamides and to explore the potential mechanism in streptozotocin (STZ)-induced diabetic rats. Diabetic rats were orally treated with 2, 4 and 8 mg per kg bw of alkylamides daily for 28 days. Alkylamides decreased the relative weight of the liver and food intake, significantly increased the relative skeletal muscle weight and significantly decreased the blood urea nitrogen levels. Insulin, insulin-like growth factor 1, total protein (TP) and albumin (ALB), globular proteins and ALB proteins/globulin protein levels in serum significantly increased. TP, RNA content and RNA/DNA ratio significantly increased in the skeletal muscle of diabetic rats. Real-time quantitative polymerase chain reaction results indicated that alkylamides significantly increased the mRNA expression of insulin receptor (InR), IGF1 and insulin-like growth factor 1 receptor (IGF1R) in the liver and skeletal muscle. Moreover, the mRNA and protein expression levels of PI3K, PKB and mTOR significantly increased, whereas those of atrogin-1, muscle ring finger 1 and FOXO in the skeletal muscle significantly decreased. Alkylamides may advance protein synthesis by the PI3K/PKB/mTOR signalling pathway and attenuate the catabolism of protein through the ubiquitin–proteasome pathway. Therefore, it was possible that alkylamides ameliorate protein metabolism disorders in diabetic rats by activating the mTOR pathway. Bioscientifica Ltd 2017-01-18 /pmc/articles/PMC5424265/ /pubmed/28100702 http://dx.doi.org/10.1530/JME-16-0218 Text en © 2017 The authors http://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Research
Ren, Tingyuan
Zhu, Yuping
Xia, Xuejuan
Ding, Yongbo
Guo, Jing
Kan, Jianquan
Zanthoxylum alkylamides ameliorate protein metabolism disorder in STZ-induced diabetic rats
title Zanthoxylum alkylamides ameliorate protein metabolism disorder in STZ-induced diabetic rats
title_full Zanthoxylum alkylamides ameliorate protein metabolism disorder in STZ-induced diabetic rats
title_fullStr Zanthoxylum alkylamides ameliorate protein metabolism disorder in STZ-induced diabetic rats
title_full_unstemmed Zanthoxylum alkylamides ameliorate protein metabolism disorder in STZ-induced diabetic rats
title_short Zanthoxylum alkylamides ameliorate protein metabolism disorder in STZ-induced diabetic rats
title_sort zanthoxylum alkylamides ameliorate protein metabolism disorder in stz-induced diabetic rats
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5424265/
https://www.ncbi.nlm.nih.gov/pubmed/28100702
http://dx.doi.org/10.1530/JME-16-0218
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