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Quantifying capture stress in free ranging European roe deer (Capreolus capreolus)
BACKGROUND: To understand and reduce the concomitant effects of trapping and handling procedures in wildlife species, it is essential to measure their physiological impact. Here, we examined individual variation in stress levels in non-anesthetized European roe deer (Capreolus capreolus), which were...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5424289/ https://www.ncbi.nlm.nih.gov/pubmed/28490331 http://dx.doi.org/10.1186/s12917-017-1045-0 |
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author | Huber, Nikolaus Vetter, Sebastian G. Evans, Alina L. Kjellander, Petter Küker, Susanne Bergvall, Ulrika A. Arnemo, Jon M. |
author_facet | Huber, Nikolaus Vetter, Sebastian G. Evans, Alina L. Kjellander, Petter Küker, Susanne Bergvall, Ulrika A. Arnemo, Jon M. |
author_sort | Huber, Nikolaus |
collection | PubMed |
description | BACKGROUND: To understand and reduce the concomitant effects of trapping and handling procedures in wildlife species, it is essential to measure their physiological impact. Here, we examined individual variation in stress levels in non-anesthetized European roe deer (Capreolus capreolus), which were captured in box traps and physically restrained for tagging, biometrics and bio-sampling. In winter 2013, we collected venous blood samples from 28 individuals during 28 capture events and evaluated standard measurements for stress (heart rate, body temperature, neutrophil to lymphocyte ratio, lactate and total cortisol). Additionally, we assessed stress using the immunological tool, Leukocyte Coping Capacity (LCC), a real-time proxy for stress measuring oxygen radical production by leukocytes. Finally, the behavioral response to handling was recorded using a scoring system. RESULTS: LCC and therefore stress levels were negatively influenced by the time animals spent in the box trap with human presence at the capture site prior to handling. In contrast, none of the classical stress measures, including total cortisol, nor the behavioral assessment, were correlated with the stressor tested (time of human presence prior to handling) and thus did not provide a clear depiction regarding the extent of the animals short-term stress response. CONCLUSIONS: Overall our study verifies the LCC as a strong method to quantify short-term stress reactions in wildlife. Moreover, our results clearly show that human presence at the trapping site prior to handling should be kept to an absolute minimum in order to reduce stress levels. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12917-017-1045-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5424289 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-54242892017-05-10 Quantifying capture stress in free ranging European roe deer (Capreolus capreolus) Huber, Nikolaus Vetter, Sebastian G. Evans, Alina L. Kjellander, Petter Küker, Susanne Bergvall, Ulrika A. Arnemo, Jon M. BMC Vet Res Research Article BACKGROUND: To understand and reduce the concomitant effects of trapping and handling procedures in wildlife species, it is essential to measure their physiological impact. Here, we examined individual variation in stress levels in non-anesthetized European roe deer (Capreolus capreolus), which were captured in box traps and physically restrained for tagging, biometrics and bio-sampling. In winter 2013, we collected venous blood samples from 28 individuals during 28 capture events and evaluated standard measurements for stress (heart rate, body temperature, neutrophil to lymphocyte ratio, lactate and total cortisol). Additionally, we assessed stress using the immunological tool, Leukocyte Coping Capacity (LCC), a real-time proxy for stress measuring oxygen radical production by leukocytes. Finally, the behavioral response to handling was recorded using a scoring system. RESULTS: LCC and therefore stress levels were negatively influenced by the time animals spent in the box trap with human presence at the capture site prior to handling. In contrast, none of the classical stress measures, including total cortisol, nor the behavioral assessment, were correlated with the stressor tested (time of human presence prior to handling) and thus did not provide a clear depiction regarding the extent of the animals short-term stress response. CONCLUSIONS: Overall our study verifies the LCC as a strong method to quantify short-term stress reactions in wildlife. Moreover, our results clearly show that human presence at the trapping site prior to handling should be kept to an absolute minimum in order to reduce stress levels. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12917-017-1045-0) contains supplementary material, which is available to authorized users. BioMed Central 2017-05-10 /pmc/articles/PMC5424289/ /pubmed/28490331 http://dx.doi.org/10.1186/s12917-017-1045-0 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Huber, Nikolaus Vetter, Sebastian G. Evans, Alina L. Kjellander, Petter Küker, Susanne Bergvall, Ulrika A. Arnemo, Jon M. Quantifying capture stress in free ranging European roe deer (Capreolus capreolus) |
title | Quantifying capture stress in free ranging European roe deer (Capreolus capreolus) |
title_full | Quantifying capture stress in free ranging European roe deer (Capreolus capreolus) |
title_fullStr | Quantifying capture stress in free ranging European roe deer (Capreolus capreolus) |
title_full_unstemmed | Quantifying capture stress in free ranging European roe deer (Capreolus capreolus) |
title_short | Quantifying capture stress in free ranging European roe deer (Capreolus capreolus) |
title_sort | quantifying capture stress in free ranging european roe deer (capreolus capreolus) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5424289/ https://www.ncbi.nlm.nih.gov/pubmed/28490331 http://dx.doi.org/10.1186/s12917-017-1045-0 |
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