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Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis)

β-catenin is a key signalling molecule in the canonical Wnt pathway, which plays a role in cell adhesion, embryogenesis and sex determination. However, little is known about its function in teleosts. We cloned and characterized the full-length β-catenin1 gene from half-smooth tongue sole (Cynoglossu...

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Autores principales: Zhu, Ying, Hu, Qiaomu, Xu, Wenteng, Li, Hailong, Guo, Hua, Meng, Liang, Wei, Min, Lu, Sheng, Shao, Changwei, Wang, Na, Yang, Guanpin, Chen, Songlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425175/
https://www.ncbi.nlm.nih.gov/pubmed/28489928
http://dx.doi.org/10.1371/journal.pone.0176122
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author Zhu, Ying
Hu, Qiaomu
Xu, Wenteng
Li, Hailong
Guo, Hua
Meng, Liang
Wei, Min
Lu, Sheng
Shao, Changwei
Wang, Na
Yang, Guanpin
Chen, Songlin
author_facet Zhu, Ying
Hu, Qiaomu
Xu, Wenteng
Li, Hailong
Guo, Hua
Meng, Liang
Wei, Min
Lu, Sheng
Shao, Changwei
Wang, Na
Yang, Guanpin
Chen, Songlin
author_sort Zhu, Ying
collection PubMed
description β-catenin is a key signalling molecule in the canonical Wnt pathway, which plays a role in cell adhesion, embryogenesis and sex determination. However, little is known about its function in teleosts. We cloned and characterized the full-length β-catenin1 gene from half-smooth tongue sole (Cynoglossus semilaevis), which was designated CS-β-catenin1. The CS-β-catenin1 cDNA consists of 2,346 nucleotides and encodes a protein with 782 amino acids. Although CS-β-catenin1 was transcribed in the gonads of both sexes, the level was significantly higher in ovaries compared to testes. Furthermore, the mRNA level of CS-β-catenin1 was significantly upregulated at 160 days and constantly increased until 2 years of age. In situ hybridization revealed that CS-β-catenin1 mRNA was mainly localized in oocyte cells, especially in stage I, II and III oocytes. When CS-β-catenin1 expression was inhibited by injection of quercetin in the ovaries, levels of CS-Figla and CS-foxl2 mRNA were significantly down-regulated, and CS-dmrt1 was up-regulated, which suggested that CS-β-catenin1 is a potential upstream gene of CS-Figla and is involved in the development of the ovaries, i.e., folliculogenesis.
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spelling pubmed-54251752017-05-15 Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis) Zhu, Ying Hu, Qiaomu Xu, Wenteng Li, Hailong Guo, Hua Meng, Liang Wei, Min Lu, Sheng Shao, Changwei Wang, Na Yang, Guanpin Chen, Songlin PLoS One Research Article β-catenin is a key signalling molecule in the canonical Wnt pathway, which plays a role in cell adhesion, embryogenesis and sex determination. However, little is known about its function in teleosts. We cloned and characterized the full-length β-catenin1 gene from half-smooth tongue sole (Cynoglossus semilaevis), which was designated CS-β-catenin1. The CS-β-catenin1 cDNA consists of 2,346 nucleotides and encodes a protein with 782 amino acids. Although CS-β-catenin1 was transcribed in the gonads of both sexes, the level was significantly higher in ovaries compared to testes. Furthermore, the mRNA level of CS-β-catenin1 was significantly upregulated at 160 days and constantly increased until 2 years of age. In situ hybridization revealed that CS-β-catenin1 mRNA was mainly localized in oocyte cells, especially in stage I, II and III oocytes. When CS-β-catenin1 expression was inhibited by injection of quercetin in the ovaries, levels of CS-Figla and CS-foxl2 mRNA were significantly down-regulated, and CS-dmrt1 was up-regulated, which suggested that CS-β-catenin1 is a potential upstream gene of CS-Figla and is involved in the development of the ovaries, i.e., folliculogenesis. Public Library of Science 2017-05-10 /pmc/articles/PMC5425175/ /pubmed/28489928 http://dx.doi.org/10.1371/journal.pone.0176122 Text en © 2017 Zhu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhu, Ying
Hu, Qiaomu
Xu, Wenteng
Li, Hailong
Guo, Hua
Meng, Liang
Wei, Min
Lu, Sheng
Shao, Changwei
Wang, Na
Yang, Guanpin
Chen, Songlin
Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis)
title Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis)
title_full Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis)
title_fullStr Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis)
title_full_unstemmed Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis)
title_short Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis)
title_sort identification and analysis of the β-catenin1 gene in half-smooth tongue sole (cynoglossus semilaevis)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425175/
https://www.ncbi.nlm.nih.gov/pubmed/28489928
http://dx.doi.org/10.1371/journal.pone.0176122
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