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Fabrication and Evaluation of Electrospun, 3D-Bioplotted, and Combination of Electrospun/3D-Bioplotted Scaffolds for Tissue Engineering Applications

Electrospun scaffolds provide a dense framework of nanofibers with pore sizes and fiber diameters that closely resemble the architecture of native extracellular matrix. However, it generates limited three-dimensional structures of relevant physiological thicknesses. 3D printing allows digitally cont...

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Autores principales: Mellor, Liliana F., Huebner, Pedro, Cai, Shaobo, Mohiti-Asli, Mahsa, Taylor, Michael A., Spang, Jeffrey, Shirwaiker, Rohan A., Loboa, Elizabeth G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425832/
https://www.ncbi.nlm.nih.gov/pubmed/28536700
http://dx.doi.org/10.1155/2017/6956794
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author Mellor, Liliana F.
Huebner, Pedro
Cai, Shaobo
Mohiti-Asli, Mahsa
Taylor, Michael A.
Spang, Jeffrey
Shirwaiker, Rohan A.
Loboa, Elizabeth G.
author_facet Mellor, Liliana F.
Huebner, Pedro
Cai, Shaobo
Mohiti-Asli, Mahsa
Taylor, Michael A.
Spang, Jeffrey
Shirwaiker, Rohan A.
Loboa, Elizabeth G.
author_sort Mellor, Liliana F.
collection PubMed
description Electrospun scaffolds provide a dense framework of nanofibers with pore sizes and fiber diameters that closely resemble the architecture of native extracellular matrix. However, it generates limited three-dimensional structures of relevant physiological thicknesses. 3D printing allows digitally controlled fabrication of three-dimensional single/multimaterial constructs with precisely ordered fiber and pore architecture in a single build. However, this approach generally lacks the ability to achieve submicron resolution features to mimic native tissue. The goal of this study was to fabricate and evaluate 3D printed, electrospun, and combination of 3D printed/electrospun scaffolds to mimic the native architecture of heterogeneous tissue. We assessed their ability to support viability and proliferation of human adipose derived stem cells (hASC). Cells had increased proliferation and high viability over 21 days on all scaffolds. We further tested implantation of stacked-electrospun scaffold versus combined electrospun/3D scaffold on a cadaveric pig knee model and found that stacked-electrospun scaffold easily delaminated during implantation while the combined scaffold was easier to implant. Our approach combining these two commonly used scaffold fabrication technologies allows for the creation of a scaffold with more close resemblance to heterogeneous tissue architecture, holding great potential for tissue engineering and regenerative medicine applications of osteochondral tissue and other heterogeneous tissues.
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spelling pubmed-54258322017-05-23 Fabrication and Evaluation of Electrospun, 3D-Bioplotted, and Combination of Electrospun/3D-Bioplotted Scaffolds for Tissue Engineering Applications Mellor, Liliana F. Huebner, Pedro Cai, Shaobo Mohiti-Asli, Mahsa Taylor, Michael A. Spang, Jeffrey Shirwaiker, Rohan A. Loboa, Elizabeth G. Biomed Res Int Research Article Electrospun scaffolds provide a dense framework of nanofibers with pore sizes and fiber diameters that closely resemble the architecture of native extracellular matrix. However, it generates limited three-dimensional structures of relevant physiological thicknesses. 3D printing allows digitally controlled fabrication of three-dimensional single/multimaterial constructs with precisely ordered fiber and pore architecture in a single build. However, this approach generally lacks the ability to achieve submicron resolution features to mimic native tissue. The goal of this study was to fabricate and evaluate 3D printed, electrospun, and combination of 3D printed/electrospun scaffolds to mimic the native architecture of heterogeneous tissue. We assessed their ability to support viability and proliferation of human adipose derived stem cells (hASC). Cells had increased proliferation and high viability over 21 days on all scaffolds. We further tested implantation of stacked-electrospun scaffold versus combined electrospun/3D scaffold on a cadaveric pig knee model and found that stacked-electrospun scaffold easily delaminated during implantation while the combined scaffold was easier to implant. Our approach combining these two commonly used scaffold fabrication technologies allows for the creation of a scaffold with more close resemblance to heterogeneous tissue architecture, holding great potential for tissue engineering and regenerative medicine applications of osteochondral tissue and other heterogeneous tissues. Hindawi 2017 2017-04-27 /pmc/articles/PMC5425832/ /pubmed/28536700 http://dx.doi.org/10.1155/2017/6956794 Text en Copyright © 2017 Liliana F. Mellor et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mellor, Liliana F.
Huebner, Pedro
Cai, Shaobo
Mohiti-Asli, Mahsa
Taylor, Michael A.
Spang, Jeffrey
Shirwaiker, Rohan A.
Loboa, Elizabeth G.
Fabrication and Evaluation of Electrospun, 3D-Bioplotted, and Combination of Electrospun/3D-Bioplotted Scaffolds for Tissue Engineering Applications
title Fabrication and Evaluation of Electrospun, 3D-Bioplotted, and Combination of Electrospun/3D-Bioplotted Scaffolds for Tissue Engineering Applications
title_full Fabrication and Evaluation of Electrospun, 3D-Bioplotted, and Combination of Electrospun/3D-Bioplotted Scaffolds for Tissue Engineering Applications
title_fullStr Fabrication and Evaluation of Electrospun, 3D-Bioplotted, and Combination of Electrospun/3D-Bioplotted Scaffolds for Tissue Engineering Applications
title_full_unstemmed Fabrication and Evaluation of Electrospun, 3D-Bioplotted, and Combination of Electrospun/3D-Bioplotted Scaffolds for Tissue Engineering Applications
title_short Fabrication and Evaluation of Electrospun, 3D-Bioplotted, and Combination of Electrospun/3D-Bioplotted Scaffolds for Tissue Engineering Applications
title_sort fabrication and evaluation of electrospun, 3d-bioplotted, and combination of electrospun/3d-bioplotted scaffolds for tissue engineering applications
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425832/
https://www.ncbi.nlm.nih.gov/pubmed/28536700
http://dx.doi.org/10.1155/2017/6956794
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