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Recombinant production and affinity purification of the FraC pore forming toxin using hexa-His tag and pET expression cassette

OBJECTIVE(S): A newly-introduced protein toxin from a sea anemone, namely fragaceatoxin C is a protein with molecular weight of 20 kDa and pore-forming capability against cell membranes has recently grasped great attentions for its function. In this study, its coding sequence cloned as a fusion prot...

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Detalles Bibliográficos
Autores principales: Imani, Mehdi, Jaliani, Hossein Zarei, Kheirandish, Mohammad Hassan, Azadpour, Mahnaz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425919/
https://www.ncbi.nlm.nih.gov/pubmed/29026495
http://dx.doi.org/10.22038/IJBMS.2017.8577