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Monitoring the Disassembly of Virus-like Particles by (19)F-NMR
[Image: see text] Virus-like particles (VLPs) are stable protein cages derived from virus coats. They have been used extensively as biomolecular platforms, e.g., nanocarriers or vaccines, but a convenient in situ technique is lacking for tracking functional status. Here, we present a simple way to m...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425944/ https://www.ncbi.nlm.nih.gov/pubmed/28350443 http://dx.doi.org/10.1021/jacs.6b11040 |
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author | Leung, Rogen L. C. Robinson, Matthew D. M. Ajabali, Alaa A. A. Karunanithy, Gogulan Lyons, Brian Raj, Ritu Raoufmoghaddam, Saeed Mohammed, Shabaz Claridge, Timothy D. W. Baldwin, Andrew J. Davis, Benjamin G. |
author_facet | Leung, Rogen L. C. Robinson, Matthew D. M. Ajabali, Alaa A. A. Karunanithy, Gogulan Lyons, Brian Raj, Ritu Raoufmoghaddam, Saeed Mohammed, Shabaz Claridge, Timothy D. W. Baldwin, Andrew J. Davis, Benjamin G. |
author_sort | Leung, Rogen L. C. |
collection | PubMed |
description | [Image: see text] Virus-like particles (VLPs) are stable protein cages derived from virus coats. They have been used extensively as biomolecular platforms, e.g., nanocarriers or vaccines, but a convenient in situ technique is lacking for tracking functional status. Here, we present a simple way to monitor disassembly of (19)F-labeled VLPs derived from bacteriophage Qβ by (19)F NMR. Analysis of resonances, under a range of conditions, allowed determination not only of the particle as fully assembled but also as disassembled, as well as detection of a degraded state upon digestion by cells. This in turn allowed mutational redesign of disassembly and testing in both bacterial and mammalian systems as a strategy for the creation of putative, targeted-VLP delivery systems. |
format | Online Article Text |
id | pubmed-5425944 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-54259442017-05-12 Monitoring the Disassembly of Virus-like Particles by (19)F-NMR Leung, Rogen L. C. Robinson, Matthew D. M. Ajabali, Alaa A. A. Karunanithy, Gogulan Lyons, Brian Raj, Ritu Raoufmoghaddam, Saeed Mohammed, Shabaz Claridge, Timothy D. W. Baldwin, Andrew J. Davis, Benjamin G. J Am Chem Soc [Image: see text] Virus-like particles (VLPs) are stable protein cages derived from virus coats. They have been used extensively as biomolecular platforms, e.g., nanocarriers or vaccines, but a convenient in situ technique is lacking for tracking functional status. Here, we present a simple way to monitor disassembly of (19)F-labeled VLPs derived from bacteriophage Qβ by (19)F NMR. Analysis of resonances, under a range of conditions, allowed determination not only of the particle as fully assembled but also as disassembled, as well as detection of a degraded state upon digestion by cells. This in turn allowed mutational redesign of disassembly and testing in both bacterial and mammalian systems as a strategy for the creation of putative, targeted-VLP delivery systems. American Chemical Society 2017-03-28 2017-04-19 /pmc/articles/PMC5425944/ /pubmed/28350443 http://dx.doi.org/10.1021/jacs.6b11040 Text en Copyright © 2017 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
spellingShingle | Leung, Rogen L. C. Robinson, Matthew D. M. Ajabali, Alaa A. A. Karunanithy, Gogulan Lyons, Brian Raj, Ritu Raoufmoghaddam, Saeed Mohammed, Shabaz Claridge, Timothy D. W. Baldwin, Andrew J. Davis, Benjamin G. Monitoring the Disassembly of Virus-like Particles by (19)F-NMR |
title | Monitoring
the Disassembly of Virus-like Particles
by (19)F-NMR |
title_full | Monitoring
the Disassembly of Virus-like Particles
by (19)F-NMR |
title_fullStr | Monitoring
the Disassembly of Virus-like Particles
by (19)F-NMR |
title_full_unstemmed | Monitoring
the Disassembly of Virus-like Particles
by (19)F-NMR |
title_short | Monitoring
the Disassembly of Virus-like Particles
by (19)F-NMR |
title_sort | monitoring
the disassembly of virus-like particles
by (19)f-nmr |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425944/ https://www.ncbi.nlm.nih.gov/pubmed/28350443 http://dx.doi.org/10.1021/jacs.6b11040 |
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