Cargando…
Interleukin 1β (+3954; −511) Genotype Polymorphism and its Association with Severe Chronic Generalized Periodontitis in the Malaysian Population
INTRODUCTION: Single-nucleotide polymorphisms (SNPs) in interleukin 1β (IL-1β) gene have been known to be associated with increased susceptibility to chronic periodontitis among various ethnic populations. SNPs are more commonly observed at loci + 3954 and − 511. The aim of this study was to evaluat...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5426139/ https://www.ncbi.nlm.nih.gov/pubmed/28566859 http://dx.doi.org/10.4103/ccd.ccd_1177_16 |
_version_ | 1783235412177715200 |
---|---|
author | Arora, Shelly Ramachandra, Srinivas Sulugodu Abdullah, Fawzia Gundavarapu, Kalyan C. |
author_facet | Arora, Shelly Ramachandra, Srinivas Sulugodu Abdullah, Fawzia Gundavarapu, Kalyan C. |
author_sort | Arora, Shelly |
collection | PubMed |
description | INTRODUCTION: Single-nucleotide polymorphisms (SNPs) in interleukin 1β (IL-1β) gene have been known to be associated with increased susceptibility to chronic periodontitis among various ethnic populations. SNPs are more commonly observed at loci + 3954 and − 511. The aim of this study was to evaluate the role of IL-1β gene polymorphism at loci +3954 and − 511, and its association with severe chronic generalized periodontitis among the ethnic Malay, Chinese, and Indians within the Malaysian population. MATERIALS AND METHODS: Saliva samples from 120 subjects (60 cases and 60 controls) in the age group of 25–50 years were collected for isolation of genetic material using Norgen technique. Clinical attachment loss of ≥5 mm was considered as severe chronic generalized periodontitis. SNP's at loci +3954 and − 511 were identified and analyzed using Kompetitive Allele Specific Polymerase Chain Reaction Genotyping System (KASP™). Differences in the allele/genotype frequencies were assessed by Chi-square test (P < 0.05). RESULTS: On the comparison between cases and controls of IL-1β genotype polymorphism (+3954 and − 511), the difference in the genotype frequencies was statistically insignificant in all the three ethnicities. The genotype frequency in both groups in all three ethnicities of the Malaysian population was similar. CONCLUSION: IL-1β genotype polymorphism at +3954 and − 511 was found to be not associated with severe chronic generalized periodontitis among the three ethnicities in Malaysia. Studies with larger sample size should be done to confirm the findings of this study. |
format | Online Article Text |
id | pubmed-5426139 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-54261392017-05-31 Interleukin 1β (+3954; −511) Genotype Polymorphism and its Association with Severe Chronic Generalized Periodontitis in the Malaysian Population Arora, Shelly Ramachandra, Srinivas Sulugodu Abdullah, Fawzia Gundavarapu, Kalyan C. Contemp Clin Dent Original Article INTRODUCTION: Single-nucleotide polymorphisms (SNPs) in interleukin 1β (IL-1β) gene have been known to be associated with increased susceptibility to chronic periodontitis among various ethnic populations. SNPs are more commonly observed at loci + 3954 and − 511. The aim of this study was to evaluate the role of IL-1β gene polymorphism at loci +3954 and − 511, and its association with severe chronic generalized periodontitis among the ethnic Malay, Chinese, and Indians within the Malaysian population. MATERIALS AND METHODS: Saliva samples from 120 subjects (60 cases and 60 controls) in the age group of 25–50 years were collected for isolation of genetic material using Norgen technique. Clinical attachment loss of ≥5 mm was considered as severe chronic generalized periodontitis. SNP's at loci +3954 and − 511 were identified and analyzed using Kompetitive Allele Specific Polymerase Chain Reaction Genotyping System (KASP™). Differences in the allele/genotype frequencies were assessed by Chi-square test (P < 0.05). RESULTS: On the comparison between cases and controls of IL-1β genotype polymorphism (+3954 and − 511), the difference in the genotype frequencies was statistically insignificant in all the three ethnicities. The genotype frequency in both groups in all three ethnicities of the Malaysian population was similar. CONCLUSION: IL-1β genotype polymorphism at +3954 and − 511 was found to be not associated with severe chronic generalized periodontitis among the three ethnicities in Malaysia. Studies with larger sample size should be done to confirm the findings of this study. Medknow Publications & Media Pvt Ltd 2017 /pmc/articles/PMC5426139/ /pubmed/28566859 http://dx.doi.org/10.4103/ccd.ccd_1177_16 Text en Copyright: © 2017 Contemporary Clinical Dentistry http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Arora, Shelly Ramachandra, Srinivas Sulugodu Abdullah, Fawzia Gundavarapu, Kalyan C. Interleukin 1β (+3954; −511) Genotype Polymorphism and its Association with Severe Chronic Generalized Periodontitis in the Malaysian Population |
title | Interleukin 1β (+3954; −511) Genotype Polymorphism and its Association with Severe Chronic Generalized Periodontitis in the Malaysian Population |
title_full | Interleukin 1β (+3954; −511) Genotype Polymorphism and its Association with Severe Chronic Generalized Periodontitis in the Malaysian Population |
title_fullStr | Interleukin 1β (+3954; −511) Genotype Polymorphism and its Association with Severe Chronic Generalized Periodontitis in the Malaysian Population |
title_full_unstemmed | Interleukin 1β (+3954; −511) Genotype Polymorphism and its Association with Severe Chronic Generalized Periodontitis in the Malaysian Population |
title_short | Interleukin 1β (+3954; −511) Genotype Polymorphism and its Association with Severe Chronic Generalized Periodontitis in the Malaysian Population |
title_sort | interleukin 1β (+3954; −511) genotype polymorphism and its association with severe chronic generalized periodontitis in the malaysian population |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5426139/ https://www.ncbi.nlm.nih.gov/pubmed/28566859 http://dx.doi.org/10.4103/ccd.ccd_1177_16 |
work_keys_str_mv | AT arorashelly interleukin1b3954511genotypepolymorphismanditsassociationwithseverechronicgeneralizedperiodontitisinthemalaysianpopulation AT ramachandrasrinivassulugodu interleukin1b3954511genotypepolymorphismanditsassociationwithseverechronicgeneralizedperiodontitisinthemalaysianpopulation AT abdullahfawzia interleukin1b3954511genotypepolymorphismanditsassociationwithseverechronicgeneralizedperiodontitisinthemalaysianpopulation AT gundavarapukalyanc interleukin1b3954511genotypepolymorphismanditsassociationwithseverechronicgeneralizedperiodontitisinthemalaysianpopulation |