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Crystal structure of thermostable alkylsulfatase SdsAP from Pseudomonas sp. S9
A novel alkylsulfatase from bacterium Pseudomonas sp. S9 (SdsAP) was identified as a thermostable alkylsulfatases (type III), which could hydrolyze the primary alkyl sulfate such as sodium dodecyl sulfate (SDS). Thus, it has a potential application of SDS biodegradation. The crystal structure of Sds...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5426288/ https://www.ncbi.nlm.nih.gov/pubmed/28442601 http://dx.doi.org/10.1042/BSR20170001 |
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author | Sun, Lifang Chen, Pu Su, Yintao Cai, Zhixiong Ruan, Lingwei Xu, Xun Wu, Yunkun |
author_facet | Sun, Lifang Chen, Pu Su, Yintao Cai, Zhixiong Ruan, Lingwei Xu, Xun Wu, Yunkun |
author_sort | Sun, Lifang |
collection | PubMed |
description | A novel alkylsulfatase from bacterium Pseudomonas sp. S9 (SdsAP) was identified as a thermostable alkylsulfatases (type III), which could hydrolyze the primary alkyl sulfate such as sodium dodecyl sulfate (SDS). Thus, it has a potential application of SDS biodegradation. The crystal structure of SdsAP has been solved to a resolution of 1.76 Å and reveals that SdsAP contains the characteristic metallo-β-lactamase-like fold domain, dimerization domain, and C-terminal sterol carrier protein type 2 (SCP-2)-like fold domain. Kinetic characterization of SdsAP to SDS by isothermal titration calorimetry (ITC) and enzymatic activity assays of constructed mutants demonstrate that Y246 and G263 are important residues for its preference for the hydrolysis of ‘primary alkyl’ chains, confirming that SdsAP is a primary alkylsulfatase. |
format | Online Article Text |
id | pubmed-5426288 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-54262882017-05-24 Crystal structure of thermostable alkylsulfatase SdsAP from Pseudomonas sp. S9 Sun, Lifang Chen, Pu Su, Yintao Cai, Zhixiong Ruan, Lingwei Xu, Xun Wu, Yunkun Biosci Rep Research Articles A novel alkylsulfatase from bacterium Pseudomonas sp. S9 (SdsAP) was identified as a thermostable alkylsulfatases (type III), which could hydrolyze the primary alkyl sulfate such as sodium dodecyl sulfate (SDS). Thus, it has a potential application of SDS biodegradation. The crystal structure of SdsAP has been solved to a resolution of 1.76 Å and reveals that SdsAP contains the characteristic metallo-β-lactamase-like fold domain, dimerization domain, and C-terminal sterol carrier protein type 2 (SCP-2)-like fold domain. Kinetic characterization of SdsAP to SDS by isothermal titration calorimetry (ITC) and enzymatic activity assays of constructed mutants demonstrate that Y246 and G263 are important residues for its preference for the hydrolysis of ‘primary alkyl’ chains, confirming that SdsAP is a primary alkylsulfatase. Portland Press Ltd. 2017-05-11 /pmc/articles/PMC5426288/ /pubmed/28442601 http://dx.doi.org/10.1042/BSR20170001 Text en © 2017 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Sun, Lifang Chen, Pu Su, Yintao Cai, Zhixiong Ruan, Lingwei Xu, Xun Wu, Yunkun Crystal structure of thermostable alkylsulfatase SdsAP from Pseudomonas sp. S9 |
title | Crystal structure of thermostable alkylsulfatase SdsAP from Pseudomonas sp. S9
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title_full | Crystal structure of thermostable alkylsulfatase SdsAP from Pseudomonas sp. S9
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title_fullStr | Crystal structure of thermostable alkylsulfatase SdsAP from Pseudomonas sp. S9
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title_full_unstemmed | Crystal structure of thermostable alkylsulfatase SdsAP from Pseudomonas sp. S9
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title_short | Crystal structure of thermostable alkylsulfatase SdsAP from Pseudomonas sp. S9
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title_sort | crystal structure of thermostable alkylsulfatase sdsap from pseudomonas sp. s9 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5426288/ https://www.ncbi.nlm.nih.gov/pubmed/28442601 http://dx.doi.org/10.1042/BSR20170001 |
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