Cargando…

AMP-activated kinase and the endogenous endocannabinoid system might contribute to antinociceptive effects of prolonged moderate caloric restriction in mice

BACKGROUND: Caloric restriction is associated with broad therapeutic potential in various diseases and an increase in health and life span. In this study, we assessed the impact of caloric restriction on acute and inflammatory nociception in mice, which were either fed ad libitum or subjected to cal...

Descripción completa

Detalles Bibliográficos
Autores principales: King-Himmelreich, Tanya S, Möser, Christine V, Wolters, Miriam C, Schmetzer, Julia, Möller, Moritz, Schreiber, Yannik, Ferreirós, Nerea, Geisslinger, Gerd, Niederberger, Ellen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5426584/
https://www.ncbi.nlm.nih.gov/pubmed/28381108
http://dx.doi.org/10.1177/1744806917703111
_version_ 1783235501582450688
author King-Himmelreich, Tanya S
Möser, Christine V
Wolters, Miriam C
Schmetzer, Julia
Möller, Moritz
Schreiber, Yannik
Ferreirós, Nerea
Geisslinger, Gerd
Niederberger, Ellen
author_facet King-Himmelreich, Tanya S
Möser, Christine V
Wolters, Miriam C
Schmetzer, Julia
Möller, Moritz
Schreiber, Yannik
Ferreirós, Nerea
Geisslinger, Gerd
Niederberger, Ellen
author_sort King-Himmelreich, Tanya S
collection PubMed
description BACKGROUND: Caloric restriction is associated with broad therapeutic potential in various diseases and an increase in health and life span. In this study, we assessed the impact of caloric restriction on acute and inflammatory nociception in mice, which were either fed ad libitum or subjected to caloric restriction with 80% of the daily average for two weeks. RESULTS: The behavioral tests revealed that inflammatory nociception in the formalin test and in zymosan-induced mechanical hypersensitivity were significantly decreased when mice underwent caloric restriction. As potential mediators of the diet-induced antinociception, we assessed genes typically induced by inflammatory stimuli, AMP-activated kinase, and the endocannabinoid system which have all already been associated with nociceptive responses. Zymosan-induced inflammatory markers such as COX-2, TNFα, IL-1β, and c-fos in the spinal cord were not altered by caloric restriction. In contrast, AMPKα2 knock-out mice showed significant differences in comparison to C57BL/6 mice and their respective wild type littermates by missing the antinociceptive effects after caloric restriction. Endocannabinoid levels of anandamide and 2-arachidonyl glyceroldetermined in serum by LC-MS/MS were not affected by either caloric restriction alone or in combination with zymosan treatment. However, cannabinoid receptor type 1 expression in the spinal cord, which was not altered by caloric restriction in control mice, was significantly increased after caloric restriction in zymosan-induced paw inflammation. Since increased cannabinoid receptor type 1 signaling might influence AMP-activated kinase activity, we analyzed effects of anandamide on AMP-activated kinase in cell culture and observed a significant activation of AMP-activated kinase. Thus, endocannabionoid-induced AMP-activated kinase activation might be involved in antinociceptive effects after caloric restriction. CONCLUSION: Our data suggest that caloric restriction has an impact on inflammatory nociception which might involve AMP-activated kinase activation and an increased activity of the endogenous endocannabinoid system by caloric restriction-induced cannabinoid receptor type 1 upregulation.
format Online
Article
Text
id pubmed-5426584
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher SAGE Publications
record_format MEDLINE/PubMed
spelling pubmed-54265842017-05-22 AMP-activated kinase and the endogenous endocannabinoid system might contribute to antinociceptive effects of prolonged moderate caloric restriction in mice King-Himmelreich, Tanya S Möser, Christine V Wolters, Miriam C Schmetzer, Julia Möller, Moritz Schreiber, Yannik Ferreirós, Nerea Geisslinger, Gerd Niederberger, Ellen Mol Pain Research Article BACKGROUND: Caloric restriction is associated with broad therapeutic potential in various diseases and an increase in health and life span. In this study, we assessed the impact of caloric restriction on acute and inflammatory nociception in mice, which were either fed ad libitum or subjected to caloric restriction with 80% of the daily average for two weeks. RESULTS: The behavioral tests revealed that inflammatory nociception in the formalin test and in zymosan-induced mechanical hypersensitivity were significantly decreased when mice underwent caloric restriction. As potential mediators of the diet-induced antinociception, we assessed genes typically induced by inflammatory stimuli, AMP-activated kinase, and the endocannabinoid system which have all already been associated with nociceptive responses. Zymosan-induced inflammatory markers such as COX-2, TNFα, IL-1β, and c-fos in the spinal cord were not altered by caloric restriction. In contrast, AMPKα2 knock-out mice showed significant differences in comparison to C57BL/6 mice and their respective wild type littermates by missing the antinociceptive effects after caloric restriction. Endocannabinoid levels of anandamide and 2-arachidonyl glyceroldetermined in serum by LC-MS/MS were not affected by either caloric restriction alone or in combination with zymosan treatment. However, cannabinoid receptor type 1 expression in the spinal cord, which was not altered by caloric restriction in control mice, was significantly increased after caloric restriction in zymosan-induced paw inflammation. Since increased cannabinoid receptor type 1 signaling might influence AMP-activated kinase activity, we analyzed effects of anandamide on AMP-activated kinase in cell culture and observed a significant activation of AMP-activated kinase. Thus, endocannabionoid-induced AMP-activated kinase activation might be involved in antinociceptive effects after caloric restriction. CONCLUSION: Our data suggest that caloric restriction has an impact on inflammatory nociception which might involve AMP-activated kinase activation and an increased activity of the endogenous endocannabinoid system by caloric restriction-induced cannabinoid receptor type 1 upregulation. SAGE Publications 2017-04-16 /pmc/articles/PMC5426584/ /pubmed/28381108 http://dx.doi.org/10.1177/1744806917703111 Text en © The Author(s) 2017 http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Research Article
King-Himmelreich, Tanya S
Möser, Christine V
Wolters, Miriam C
Schmetzer, Julia
Möller, Moritz
Schreiber, Yannik
Ferreirós, Nerea
Geisslinger, Gerd
Niederberger, Ellen
AMP-activated kinase and the endogenous endocannabinoid system might contribute to antinociceptive effects of prolonged moderate caloric restriction in mice
title AMP-activated kinase and the endogenous endocannabinoid system might contribute to antinociceptive effects of prolonged moderate caloric restriction in mice
title_full AMP-activated kinase and the endogenous endocannabinoid system might contribute to antinociceptive effects of prolonged moderate caloric restriction in mice
title_fullStr AMP-activated kinase and the endogenous endocannabinoid system might contribute to antinociceptive effects of prolonged moderate caloric restriction in mice
title_full_unstemmed AMP-activated kinase and the endogenous endocannabinoid system might contribute to antinociceptive effects of prolonged moderate caloric restriction in mice
title_short AMP-activated kinase and the endogenous endocannabinoid system might contribute to antinociceptive effects of prolonged moderate caloric restriction in mice
title_sort amp-activated kinase and the endogenous endocannabinoid system might contribute to antinociceptive effects of prolonged moderate caloric restriction in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5426584/
https://www.ncbi.nlm.nih.gov/pubmed/28381108
http://dx.doi.org/10.1177/1744806917703111
work_keys_str_mv AT kinghimmelreichtanyas ampactivatedkinaseandtheendogenousendocannabinoidsystemmightcontributetoantinociceptiveeffectsofprolongedmoderatecaloricrestrictioninmice
AT moserchristinev ampactivatedkinaseandtheendogenousendocannabinoidsystemmightcontributetoantinociceptiveeffectsofprolongedmoderatecaloricrestrictioninmice
AT woltersmiriamc ampactivatedkinaseandtheendogenousendocannabinoidsystemmightcontributetoantinociceptiveeffectsofprolongedmoderatecaloricrestrictioninmice
AT schmetzerjulia ampactivatedkinaseandtheendogenousendocannabinoidsystemmightcontributetoantinociceptiveeffectsofprolongedmoderatecaloricrestrictioninmice
AT mollermoritz ampactivatedkinaseandtheendogenousendocannabinoidsystemmightcontributetoantinociceptiveeffectsofprolongedmoderatecaloricrestrictioninmice
AT schreiberyannik ampactivatedkinaseandtheendogenousendocannabinoidsystemmightcontributetoantinociceptiveeffectsofprolongedmoderatecaloricrestrictioninmice
AT ferreirosnerea ampactivatedkinaseandtheendogenousendocannabinoidsystemmightcontributetoantinociceptiveeffectsofprolongedmoderatecaloricrestrictioninmice
AT geisslingergerd ampactivatedkinaseandtheendogenousendocannabinoidsystemmightcontributetoantinociceptiveeffectsofprolongedmoderatecaloricrestrictioninmice
AT niederbergerellen ampactivatedkinaseandtheendogenousendocannabinoidsystemmightcontributetoantinociceptiveeffectsofprolongedmoderatecaloricrestrictioninmice