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Protective effects and functional mechanisms of Lactobacillus gasseri SBT2055 against oxidative stress

Lactobacillus gasseri SBT2055 (LG2055) is one of the probiotic lactic acid bacteria. Recently, we demonstrated that feeding with LG2055 extended the lifespan of Caenorhabditis elegans and that the prolongevity effect was dependent upon the regulation of oxidative stress response. In this study, we a...

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Autores principales: Kobatake, Eiji, Nakagawa, Hisako, Seki, Takahiro, Miyazaki, Tadaaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5426657/
https://www.ncbi.nlm.nih.gov/pubmed/28493927
http://dx.doi.org/10.1371/journal.pone.0177106
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author Kobatake, Eiji
Nakagawa, Hisako
Seki, Takahiro
Miyazaki, Tadaaki
author_facet Kobatake, Eiji
Nakagawa, Hisako
Seki, Takahiro
Miyazaki, Tadaaki
author_sort Kobatake, Eiji
collection PubMed
description Lactobacillus gasseri SBT2055 (LG2055) is one of the probiotic lactic acid bacteria. Recently, we demonstrated that feeding with LG2055 extended the lifespan of Caenorhabditis elegans and that the prolongevity effect was dependent upon the regulation of oxidative stress response. In this study, we assessed whether LG2055 regulated the oxidative stress response of mammalian cells. In NIH-3T3 cells and primary mouse embryonic fibroblast cells, low cell proliferation rates and high reactive oxygen species levels were observed following paraquat treatment. LG2055 treatment suppressed these responses in paraquat-treated cells, indicating that LG2055 protected against oxidative stress in mammalian cells. The mRNA expression of oxidative stress-related genes, total nuclear factor-erythroid-2-related factor 2 (Nrf2) protein levels, and the nuclear translocation of Nrf2 were increased by LG2055 treatment. These results suggested that the Nrf2-antioxidant response element (ARE) signaling pathway was activated by LG2055. Furthermore, c-Jun NH(2)-terminal kinase (JNK) was activated by LG2055 treatment and the inhibition of JNK suppressed the activation of the Nrf2-ARE signaling pathway in LG2055-treated cells. Together, these findings suggest that LG2055 activated the Nrf2-ARE signaling pathway by JNK activation, thus strengthening the defense system against oxidative stress in mammalian cells.
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spelling pubmed-54266572017-05-25 Protective effects and functional mechanisms of Lactobacillus gasseri SBT2055 against oxidative stress Kobatake, Eiji Nakagawa, Hisako Seki, Takahiro Miyazaki, Tadaaki PLoS One Research Article Lactobacillus gasseri SBT2055 (LG2055) is one of the probiotic lactic acid bacteria. Recently, we demonstrated that feeding with LG2055 extended the lifespan of Caenorhabditis elegans and that the prolongevity effect was dependent upon the regulation of oxidative stress response. In this study, we assessed whether LG2055 regulated the oxidative stress response of mammalian cells. In NIH-3T3 cells and primary mouse embryonic fibroblast cells, low cell proliferation rates and high reactive oxygen species levels were observed following paraquat treatment. LG2055 treatment suppressed these responses in paraquat-treated cells, indicating that LG2055 protected against oxidative stress in mammalian cells. The mRNA expression of oxidative stress-related genes, total nuclear factor-erythroid-2-related factor 2 (Nrf2) protein levels, and the nuclear translocation of Nrf2 were increased by LG2055 treatment. These results suggested that the Nrf2-antioxidant response element (ARE) signaling pathway was activated by LG2055. Furthermore, c-Jun NH(2)-terminal kinase (JNK) was activated by LG2055 treatment and the inhibition of JNK suppressed the activation of the Nrf2-ARE signaling pathway in LG2055-treated cells. Together, these findings suggest that LG2055 activated the Nrf2-ARE signaling pathway by JNK activation, thus strengthening the defense system against oxidative stress in mammalian cells. Public Library of Science 2017-05-11 /pmc/articles/PMC5426657/ /pubmed/28493927 http://dx.doi.org/10.1371/journal.pone.0177106 Text en © 2017 Kobatake et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kobatake, Eiji
Nakagawa, Hisako
Seki, Takahiro
Miyazaki, Tadaaki
Protective effects and functional mechanisms of Lactobacillus gasseri SBT2055 against oxidative stress
title Protective effects and functional mechanisms of Lactobacillus gasseri SBT2055 against oxidative stress
title_full Protective effects and functional mechanisms of Lactobacillus gasseri SBT2055 against oxidative stress
title_fullStr Protective effects and functional mechanisms of Lactobacillus gasseri SBT2055 against oxidative stress
title_full_unstemmed Protective effects and functional mechanisms of Lactobacillus gasseri SBT2055 against oxidative stress
title_short Protective effects and functional mechanisms of Lactobacillus gasseri SBT2055 against oxidative stress
title_sort protective effects and functional mechanisms of lactobacillus gasseri sbt2055 against oxidative stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5426657/
https://www.ncbi.nlm.nih.gov/pubmed/28493927
http://dx.doi.org/10.1371/journal.pone.0177106
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