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Isolation and characterization of phthalates from Brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest

Bacteria belonging to the family Brevibacterieae are ubiquitous Gram positive organisms that are responsible for the feet odour and cheese aroma. Brevibacterium mcbrellneri is a relatively new member belonging to Brevibacterieae. In the current manuscript we discuss isolation of biologically active...

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Autores principales: Rajamanikyam, Maheshwari, Vadlapudi, Varahalarao, Parvathaneni, Sai Prathima, Koude, Dhevendar, Sripadi, Prabhakar, Misra, Sunil, Amanchy, Ramars, Upadhyayula, Suryanarayana Murty
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Leibniz Research Centre for Working Environment and Human Factors 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5427467/
https://www.ncbi.nlm.nih.gov/pubmed/28507481
http://dx.doi.org/10.17179/excli2017-145
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author Rajamanikyam, Maheshwari
Vadlapudi, Varahalarao
Parvathaneni, Sai Prathima
Koude, Dhevendar
Sripadi, Prabhakar
Misra, Sunil
Amanchy, Ramars
Upadhyayula, Suryanarayana Murty
author_facet Rajamanikyam, Maheshwari
Vadlapudi, Varahalarao
Parvathaneni, Sai Prathima
Koude, Dhevendar
Sripadi, Prabhakar
Misra, Sunil
Amanchy, Ramars
Upadhyayula, Suryanarayana Murty
author_sort Rajamanikyam, Maheshwari
collection PubMed
description Bacteria belonging to the family Brevibacterieae are ubiquitous Gram positive organisms that are responsible for the feet odour and cheese aroma. Brevibacterium mcbrellneri is a relatively new member belonging to Brevibacterieae. In the current manuscript we discuss isolation of biologically active metabolites from Brevibacterium mcbrellneri. Two aromatic esters were isolated from Brevibacterium mcbrellneri by “Bioassay guided fractionation strategy” and identified as di-(2-ethylhexyl) phthalate and dibutyl phthalate by chemical characterization using biophysical techniques. The phthalate compounds show broad spectrum antibacterial activity and mosquito larvicidal activity. Mosquito larvicidal activity has been attributed to inhibition of acetylcholinesterase enzyme activity. These compounds were found to be cytotoxic in multiple cell lines causing cell cycle arrest in G1 phase.
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spelling pubmed-54274672017-05-15 Isolation and characterization of phthalates from Brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest Rajamanikyam, Maheshwari Vadlapudi, Varahalarao Parvathaneni, Sai Prathima Koude, Dhevendar Sripadi, Prabhakar Misra, Sunil Amanchy, Ramars Upadhyayula, Suryanarayana Murty EXCLI J Original Article Bacteria belonging to the family Brevibacterieae are ubiquitous Gram positive organisms that are responsible for the feet odour and cheese aroma. Brevibacterium mcbrellneri is a relatively new member belonging to Brevibacterieae. In the current manuscript we discuss isolation of biologically active metabolites from Brevibacterium mcbrellneri. Two aromatic esters were isolated from Brevibacterium mcbrellneri by “Bioassay guided fractionation strategy” and identified as di-(2-ethylhexyl) phthalate and dibutyl phthalate by chemical characterization using biophysical techniques. The phthalate compounds show broad spectrum antibacterial activity and mosquito larvicidal activity. Mosquito larvicidal activity has been attributed to inhibition of acetylcholinesterase enzyme activity. These compounds were found to be cytotoxic in multiple cell lines causing cell cycle arrest in G1 phase. Leibniz Research Centre for Working Environment and Human Factors 2017-03-24 /pmc/articles/PMC5427467/ /pubmed/28507481 http://dx.doi.org/10.17179/excli2017-145 Text en Copyright © 2017 Rajamanikyam et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/) You are free to copy, distribute and transmit the work, provided the original author and source are credited.
spellingShingle Original Article
Rajamanikyam, Maheshwari
Vadlapudi, Varahalarao
Parvathaneni, Sai Prathima
Koude, Dhevendar
Sripadi, Prabhakar
Misra, Sunil
Amanchy, Ramars
Upadhyayula, Suryanarayana Murty
Isolation and characterization of phthalates from Brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest
title Isolation and characterization of phthalates from Brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest
title_full Isolation and characterization of phthalates from Brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest
title_fullStr Isolation and characterization of phthalates from Brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest
title_full_unstemmed Isolation and characterization of phthalates from Brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest
title_short Isolation and characterization of phthalates from Brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest
title_sort isolation and characterization of phthalates from brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5427467/
https://www.ncbi.nlm.nih.gov/pubmed/28507481
http://dx.doi.org/10.17179/excli2017-145
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