Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable Phenotype

CRISPR/Cas9 genome engineering strategies allow the directed modification of the Caenorhabditis elegans genome to introduce point mutations, generate knock-out mutants, and insert coding sequences for epitope or fluorescent tags. Three practical aspects, however, complicate such experiments. First,...

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Detalles Bibliográficos
Autores principales: El Mouridi, Sonia, Lecroisey, Claire, Tardy, Philippe, Mercier, Marine, Leclercq-Blondel, Alice, Zariohi, Nora, Boulin, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2017
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5427500/
https://www.ncbi.nlm.nih.gov/pubmed/28280211
http://dx.doi.org/10.1534/g3.117.040824

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5427500/
https://www.ncbi.nlm.nih.gov/pubmed/28280211
http://dx.doi.org/10.1534/g3.117.040824

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