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Expression and function of HSP110 family in mouse testis after vasectomy

HSP110 functions to protect cells, tissues, and organs from noxious conditions. Vasectomy induces apoptosis in the testis; however, little is known about the reason leading to this outcome. The aim of the present study was to evaluate the expression and function of HSP110 in mouse testis after vasec...

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Autores principales: Du, Zhen-Ning, Rong, Cheng-Ting, Hui, Shi, Peng, Zhu, Jin, Shao-Hua, Li, Shi-Jia, Wang, Hai-Yan, Li, Jian-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5427794/
https://www.ncbi.nlm.nih.gov/pubmed/26952955
http://dx.doi.org/10.4103/1008-682X.164197
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author Du, Zhen-Ning
Rong, Cheng-Ting
Hui, Shi
Peng, Zhu
Jin, Shao-Hua
Li, Shi-Jia
Wang, Hai-Yan
Li, Jian-Yuan
author_facet Du, Zhen-Ning
Rong, Cheng-Ting
Hui, Shi
Peng, Zhu
Jin, Shao-Hua
Li, Shi-Jia
Wang, Hai-Yan
Li, Jian-Yuan
author_sort Du, Zhen-Ning
collection PubMed
description HSP110 functions to protect cells, tissues, and organs from noxious conditions. Vasectomy induces apoptosis in the testis; however, little is known about the reason leading to this outcome. The aim of the present study was to evaluate the expression and function of HSP110 in mouse testis after vasectomy. Following bilateral vasectomy, we used fluorescent Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) to detect apoptosis, Western blotting and immunohistochemistry to examine HSP110 expression and localization. Serum antisperm antibody (AsAb) and testosterone were measured by Enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay, respectively. Expression of endoplasmic reticulum stress (ERS) sensors and downstream signaling components was measured by Reverse Transcription-Polymerase Chain Reaction (RT-PCR), and the phosphorylation of eIF2α and JNK was detected by Western blotting. Vasectomy induced morphologic changes, increased apoptosis in the testis, increased serum AsAb, and decreased testosterone levels. After vasectomy, ORP150 mRNA level was increased first and then decreased, Bcl-2 was decreased, and the expression of HSPA4l, GRP78, GADD153, PERK, ATF6, IRE-1, XBP-1s, Bax, Bak, and caspases and the phosphorylation of eIF2α and JNK were increased. We present that an ER stress-mediated pathway is activated and involved in apoptosis in the testis after vasectomy. HSPA4l and ORP150 may play important roles in maintaining the normal structure and function of testis.
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spelling pubmed-54277942017-05-26 Expression and function of HSP110 family in mouse testis after vasectomy Du, Zhen-Ning Rong, Cheng-Ting Hui, Shi Peng, Zhu Jin, Shao-Hua Li, Shi-Jia Wang, Hai-Yan Li, Jian-Yuan Asian J Androl Original Article HSP110 functions to protect cells, tissues, and organs from noxious conditions. Vasectomy induces apoptosis in the testis; however, little is known about the reason leading to this outcome. The aim of the present study was to evaluate the expression and function of HSP110 in mouse testis after vasectomy. Following bilateral vasectomy, we used fluorescent Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) to detect apoptosis, Western blotting and immunohistochemistry to examine HSP110 expression and localization. Serum antisperm antibody (AsAb) and testosterone were measured by Enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay, respectively. Expression of endoplasmic reticulum stress (ERS) sensors and downstream signaling components was measured by Reverse Transcription-Polymerase Chain Reaction (RT-PCR), and the phosphorylation of eIF2α and JNK was detected by Western blotting. Vasectomy induced morphologic changes, increased apoptosis in the testis, increased serum AsAb, and decreased testosterone levels. After vasectomy, ORP150 mRNA level was increased first and then decreased, Bcl-2 was decreased, and the expression of HSPA4l, GRP78, GADD153, PERK, ATF6, IRE-1, XBP-1s, Bax, Bak, and caspases and the phosphorylation of eIF2α and JNK were increased. We present that an ER stress-mediated pathway is activated and involved in apoptosis in the testis after vasectomy. HSPA4l and ORP150 may play important roles in maintaining the normal structure and function of testis. Medknow Publications & Media Pvt Ltd 2017 2016-03-04 /pmc/articles/PMC5427794/ /pubmed/26952955 http://dx.doi.org/10.4103/1008-682X.164197 Text en Copyright: © The Author(s)(2017) http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Du, Zhen-Ning
Rong, Cheng-Ting
Hui, Shi
Peng, Zhu
Jin, Shao-Hua
Li, Shi-Jia
Wang, Hai-Yan
Li, Jian-Yuan
Expression and function of HSP110 family in mouse testis after vasectomy
title Expression and function of HSP110 family in mouse testis after vasectomy
title_full Expression and function of HSP110 family in mouse testis after vasectomy
title_fullStr Expression and function of HSP110 family in mouse testis after vasectomy
title_full_unstemmed Expression and function of HSP110 family in mouse testis after vasectomy
title_short Expression and function of HSP110 family in mouse testis after vasectomy
title_sort expression and function of hsp110 family in mouse testis after vasectomy
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5427794/
https://www.ncbi.nlm.nih.gov/pubmed/26952955
http://dx.doi.org/10.4103/1008-682X.164197
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