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Community-Associated Staphylococcus aureus from Sub-Saharan Africa and Germany: A Cross-Sectional Geographic Correlation Study
Clonal clusters and gene repertoires of Staphylococcus aureus are essential to understand disease and are well characterized in industrialized countries but poorly analysed in developing regions. The objective of this study was to compare the molecular-epidemiologic profiles of S. aureus isolates fr...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428059/ https://www.ncbi.nlm.nih.gov/pubmed/28273954 http://dx.doi.org/10.1038/s41598-017-00214-8 |
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author | Ruffing, Ulla Alabi, Abraham Kazimoto, Theckla Vubil, Delfino C. Akulenko, Ruslan Abdulla, Salim Alonso, Pedro Bischoff, Markus Germann, Anja Grobusch, Martin P. Helms, Volkhard Hoffmann, Jonas Kern, Winfried V. Kremsner, Peter G. Mandomando, Inacio Mellmann, Alexander Peters, Georg Schaumburg, Frieder Schubert, Sabine Strauß, Lena Tanner, Marcel Briesen, Hagen von Wende, Laura Müller, Lutz von Herrmann, Mathias |
author_facet | Ruffing, Ulla Alabi, Abraham Kazimoto, Theckla Vubil, Delfino C. Akulenko, Ruslan Abdulla, Salim Alonso, Pedro Bischoff, Markus Germann, Anja Grobusch, Martin P. Helms, Volkhard Hoffmann, Jonas Kern, Winfried V. Kremsner, Peter G. Mandomando, Inacio Mellmann, Alexander Peters, Georg Schaumburg, Frieder Schubert, Sabine Strauß, Lena Tanner, Marcel Briesen, Hagen von Wende, Laura Müller, Lutz von Herrmann, Mathias |
author_sort | Ruffing, Ulla |
collection | PubMed |
description | Clonal clusters and gene repertoires of Staphylococcus aureus are essential to understand disease and are well characterized in industrialized countries but poorly analysed in developing regions. The objective of this study was to compare the molecular-epidemiologic profiles of S. aureus isolates from Sub-Saharan Africa and Germany. S. aureus isolates from 600 staphylococcal carriers and 600 patients with community-associated staphylococcal disease were characterized by DNA hybridization, clonal complex (CC) attribution, and principal component (PCA)-based gene repertoire analysis. 73% of all CCs identified representing 77% of the isolates contained in these CCs were predominant in either African or German region. Significant differences between African versus German isolates were found for alleles encoding the accessory gene regulator type, enterotoxins, the Panton-Valentine leukocidin, immune evasion gene cluster, and adhesins. PCA in conjunction with silhouette analysis distinguished nine separable PCA clusters, with five clusters primarily comprising of African and two clusters of German isolates. Significant differences between S. aureus lineages in Africa and Germany may be a clue to explain the apparent difference in disease between tropical/(so-called) developing and temperate/industrialized regions. In low-resource countries further clinical-epidemiologic research is warranted not only for neglected tropical diseases but also for major bacterial infections. |
format | Online Article Text |
id | pubmed-5428059 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54280592017-05-15 Community-Associated Staphylococcus aureus from Sub-Saharan Africa and Germany: A Cross-Sectional Geographic Correlation Study Ruffing, Ulla Alabi, Abraham Kazimoto, Theckla Vubil, Delfino C. Akulenko, Ruslan Abdulla, Salim Alonso, Pedro Bischoff, Markus Germann, Anja Grobusch, Martin P. Helms, Volkhard Hoffmann, Jonas Kern, Winfried V. Kremsner, Peter G. Mandomando, Inacio Mellmann, Alexander Peters, Georg Schaumburg, Frieder Schubert, Sabine Strauß, Lena Tanner, Marcel Briesen, Hagen von Wende, Laura Müller, Lutz von Herrmann, Mathias Sci Rep Article Clonal clusters and gene repertoires of Staphylococcus aureus are essential to understand disease and are well characterized in industrialized countries but poorly analysed in developing regions. The objective of this study was to compare the molecular-epidemiologic profiles of S. aureus isolates from Sub-Saharan Africa and Germany. S. aureus isolates from 600 staphylococcal carriers and 600 patients with community-associated staphylococcal disease were characterized by DNA hybridization, clonal complex (CC) attribution, and principal component (PCA)-based gene repertoire analysis. 73% of all CCs identified representing 77% of the isolates contained in these CCs were predominant in either African or German region. Significant differences between African versus German isolates were found for alleles encoding the accessory gene regulator type, enterotoxins, the Panton-Valentine leukocidin, immune evasion gene cluster, and adhesins. PCA in conjunction with silhouette analysis distinguished nine separable PCA clusters, with five clusters primarily comprising of African and two clusters of German isolates. Significant differences between S. aureus lineages in Africa and Germany may be a clue to explain the apparent difference in disease between tropical/(so-called) developing and temperate/industrialized regions. In low-resource countries further clinical-epidemiologic research is warranted not only for neglected tropical diseases but also for major bacterial infections. Nature Publishing Group UK 2017-03-13 /pmc/articles/PMC5428059/ /pubmed/28273954 http://dx.doi.org/10.1038/s41598-017-00214-8 Text en © The Author(s) 2017 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Ruffing, Ulla Alabi, Abraham Kazimoto, Theckla Vubil, Delfino C. Akulenko, Ruslan Abdulla, Salim Alonso, Pedro Bischoff, Markus Germann, Anja Grobusch, Martin P. Helms, Volkhard Hoffmann, Jonas Kern, Winfried V. Kremsner, Peter G. Mandomando, Inacio Mellmann, Alexander Peters, Georg Schaumburg, Frieder Schubert, Sabine Strauß, Lena Tanner, Marcel Briesen, Hagen von Wende, Laura Müller, Lutz von Herrmann, Mathias Community-Associated Staphylococcus aureus from Sub-Saharan Africa and Germany: A Cross-Sectional Geographic Correlation Study |
title | Community-Associated Staphylococcus aureus from Sub-Saharan Africa and Germany: A Cross-Sectional Geographic Correlation Study |
title_full | Community-Associated Staphylococcus aureus from Sub-Saharan Africa and Germany: A Cross-Sectional Geographic Correlation Study |
title_fullStr | Community-Associated Staphylococcus aureus from Sub-Saharan Africa and Germany: A Cross-Sectional Geographic Correlation Study |
title_full_unstemmed | Community-Associated Staphylococcus aureus from Sub-Saharan Africa and Germany: A Cross-Sectional Geographic Correlation Study |
title_short | Community-Associated Staphylococcus aureus from Sub-Saharan Africa and Germany: A Cross-Sectional Geographic Correlation Study |
title_sort | community-associated staphylococcus aureus from sub-saharan africa and germany: a cross-sectional geographic correlation study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428059/ https://www.ncbi.nlm.nih.gov/pubmed/28273954 http://dx.doi.org/10.1038/s41598-017-00214-8 |
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