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Arterial graft with elastic layer structure grown from cells

Shortage of autologous blood vessel sources and disadvantages of synthetic grafts have increased interest in the development of tissue-engineered vascular grafts. However, tunica media, which comprises layered elastic laminae, largely determines arterial elasticity, and is difficult to synthesize. H...

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Autores principales: Yokoyama, Utako, Tonooka, Yuta, Koretake, Ryoma, Akimoto, Taisuke, Gonda, Yuki, Saito, Junichi, Umemura, Masanari, Fujita, Takayuki, Sakuma, Shinya, Arai, Fumihito, Kaneko, Makoto, Ishikawa, Yoshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428065/
https://www.ncbi.nlm.nih.gov/pubmed/28273941
http://dx.doi.org/10.1038/s41598-017-00237-1
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author Yokoyama, Utako
Tonooka, Yuta
Koretake, Ryoma
Akimoto, Taisuke
Gonda, Yuki
Saito, Junichi
Umemura, Masanari
Fujita, Takayuki
Sakuma, Shinya
Arai, Fumihito
Kaneko, Makoto
Ishikawa, Yoshihiro
author_facet Yokoyama, Utako
Tonooka, Yuta
Koretake, Ryoma
Akimoto, Taisuke
Gonda, Yuki
Saito, Junichi
Umemura, Masanari
Fujita, Takayuki
Sakuma, Shinya
Arai, Fumihito
Kaneko, Makoto
Ishikawa, Yoshihiro
author_sort Yokoyama, Utako
collection PubMed
description Shortage of autologous blood vessel sources and disadvantages of synthetic grafts have increased interest in the development of tissue-engineered vascular grafts. However, tunica media, which comprises layered elastic laminae, largely determines arterial elasticity, and is difficult to synthesize. Here, we describe a method for fabrication of arterial grafts with elastic layer structure from cultured human vascular SMCs by periodic exposure to extremely high hydrostatic pressure (HP) during repeated cell seeding. Repeated slow cycles (0.002 Hz) between 110 and 180 kPa increased stress-fiber polymerization and fibronectin fibrillogenesis on SMCs, which is required for elastic fiber formation. To fabricate arterial grafts, seeding of rat vascular SMCs and exposure to the periodic HP were repeated alternatively ten times. The obtained medial grafts were highly elastic and tensile rupture strength was 1451 ± 159 mmHg, in which elastic fibers were abundantly formed. The patch medial grafts were sutured at the rat aorta and found to be completely patent and endothelialized after 2.5 months, although tubular medial constructs implanted in rats as interpositional aortic grafts withstood arterial blood pressure only in early acute phase. This novel organized self-assembly method would enable mass production of scaffold-free arterial grafts in vitro and have potential therapeutic applications for cardiovascular diseases.
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spelling pubmed-54280652017-05-15 Arterial graft with elastic layer structure grown from cells Yokoyama, Utako Tonooka, Yuta Koretake, Ryoma Akimoto, Taisuke Gonda, Yuki Saito, Junichi Umemura, Masanari Fujita, Takayuki Sakuma, Shinya Arai, Fumihito Kaneko, Makoto Ishikawa, Yoshihiro Sci Rep Article Shortage of autologous blood vessel sources and disadvantages of synthetic grafts have increased interest in the development of tissue-engineered vascular grafts. However, tunica media, which comprises layered elastic laminae, largely determines arterial elasticity, and is difficult to synthesize. Here, we describe a method for fabrication of arterial grafts with elastic layer structure from cultured human vascular SMCs by periodic exposure to extremely high hydrostatic pressure (HP) during repeated cell seeding. Repeated slow cycles (0.002 Hz) between 110 and 180 kPa increased stress-fiber polymerization and fibronectin fibrillogenesis on SMCs, which is required for elastic fiber formation. To fabricate arterial grafts, seeding of rat vascular SMCs and exposure to the periodic HP were repeated alternatively ten times. The obtained medial grafts were highly elastic and tensile rupture strength was 1451 ± 159 mmHg, in which elastic fibers were abundantly formed. The patch medial grafts were sutured at the rat aorta and found to be completely patent and endothelialized after 2.5 months, although tubular medial constructs implanted in rats as interpositional aortic grafts withstood arterial blood pressure only in early acute phase. This novel organized self-assembly method would enable mass production of scaffold-free arterial grafts in vitro and have potential therapeutic applications for cardiovascular diseases. Nature Publishing Group UK 2017-03-10 /pmc/articles/PMC5428065/ /pubmed/28273941 http://dx.doi.org/10.1038/s41598-017-00237-1 Text en © The Author(s) 2017 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Yokoyama, Utako
Tonooka, Yuta
Koretake, Ryoma
Akimoto, Taisuke
Gonda, Yuki
Saito, Junichi
Umemura, Masanari
Fujita, Takayuki
Sakuma, Shinya
Arai, Fumihito
Kaneko, Makoto
Ishikawa, Yoshihiro
Arterial graft with elastic layer structure grown from cells
title Arterial graft with elastic layer structure grown from cells
title_full Arterial graft with elastic layer structure grown from cells
title_fullStr Arterial graft with elastic layer structure grown from cells
title_full_unstemmed Arterial graft with elastic layer structure grown from cells
title_short Arterial graft with elastic layer structure grown from cells
title_sort arterial graft with elastic layer structure grown from cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428065/
https://www.ncbi.nlm.nih.gov/pubmed/28273941
http://dx.doi.org/10.1038/s41598-017-00237-1
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