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miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2
Many reports suggest that the discovery of microRNAs (miRNAs) might provide a novel therapeutical target for many diseases, even of human cancers; however, there are no reports on the role of miR-597 in human cancers. In the present study, by detecting mRNA expression with qRT-PCR, compared with the...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428280/ https://www.ncbi.nlm.nih.gov/pubmed/28393251 http://dx.doi.org/10.3892/or.2017.5558 |
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author | He, Jiangtu Mai, Jieying Li, Yan Chen, Linyi Xu, Hui Zhu, Xiaofei Pan, Qiuhui |
author_facet | He, Jiangtu Mai, Jieying Li, Yan Chen, Linyi Xu, Hui Zhu, Xiaofei Pan, Qiuhui |
author_sort | He, Jiangtu |
collection | PubMed |
description | Many reports suggest that the discovery of microRNAs (miRNAs) might provide a novel therapeutical target for many diseases, even of human cancers; however, there are no reports on the role of miR-597 in human cancers. In the present study, by detecting mRNA expression with qRT-PCR, compared with the adjacent normal tissues we found that miR-597 was significantly downregulated in breast cancer tissues. By using the MTT assay, the cell wound-healing assay and the cell invasion assay, we demonstrated that miR-597 mimics were able to suppress breast cancer cell proliferation, migration and invasion. Additionally, with flow cytometry, we found that miR-597 influenced the growth of breast cancer cells through regulating the G1-S phase transition. Furthermore, we identified one binding site for miR-597 at the 3′UTR of the FOSL2 gene, using bioinformatics methods and the luciferase reporter assay, it was confirmed that FOSL2 was a direct target of miR-597. Moreover, overexpression of FOSL2 in MDA-MB-231 and SK-BR-3 cells can block the vast majority of the miR-597 roles, suggesting that miR-597 acts as a tumor suppressor in breast cancer cells by the downregulation of FOSL2. Additionally, we also found a negative correlation between the expression of FOSL2 and miR-597 in the tumor samples. This new regulatory mechanism in breast cancer may provide another method for diagnosis and therapy. |
format | Online Article Text |
id | pubmed-5428280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-54282802017-05-15 miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2 He, Jiangtu Mai, Jieying Li, Yan Chen, Linyi Xu, Hui Zhu, Xiaofei Pan, Qiuhui Oncol Rep Articles Many reports suggest that the discovery of microRNAs (miRNAs) might provide a novel therapeutical target for many diseases, even of human cancers; however, there are no reports on the role of miR-597 in human cancers. In the present study, by detecting mRNA expression with qRT-PCR, compared with the adjacent normal tissues we found that miR-597 was significantly downregulated in breast cancer tissues. By using the MTT assay, the cell wound-healing assay and the cell invasion assay, we demonstrated that miR-597 mimics were able to suppress breast cancer cell proliferation, migration and invasion. Additionally, with flow cytometry, we found that miR-597 influenced the growth of breast cancer cells through regulating the G1-S phase transition. Furthermore, we identified one binding site for miR-597 at the 3′UTR of the FOSL2 gene, using bioinformatics methods and the luciferase reporter assay, it was confirmed that FOSL2 was a direct target of miR-597. Moreover, overexpression of FOSL2 in MDA-MB-231 and SK-BR-3 cells can block the vast majority of the miR-597 roles, suggesting that miR-597 acts as a tumor suppressor in breast cancer cells by the downregulation of FOSL2. Additionally, we also found a negative correlation between the expression of FOSL2 and miR-597 in the tumor samples. This new regulatory mechanism in breast cancer may provide another method for diagnosis and therapy. D.A. Spandidos 2017-05 2017-04-05 /pmc/articles/PMC5428280/ /pubmed/28393251 http://dx.doi.org/10.3892/or.2017.5558 Text en Copyright: © He et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles He, Jiangtu Mai, Jieying Li, Yan Chen, Linyi Xu, Hui Zhu, Xiaofei Pan, Qiuhui miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2 |
title | miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2 |
title_full | miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2 |
title_fullStr | miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2 |
title_full_unstemmed | miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2 |
title_short | miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2 |
title_sort | mir-597 inhibits breast cancer cell proliferation, migration and invasion through fosl2 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428280/ https://www.ncbi.nlm.nih.gov/pubmed/28393251 http://dx.doi.org/10.3892/or.2017.5558 |
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