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The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs

Bone marrow mesenchymal stem cells (BMSCs) are a good candidate for tissue engineering and clinical application. One of the challenges in its cell therapy is how to quickly obtain an adequate number of seed cells and meanwhile maintain suitable differentiation potential. In this study we combined th...

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Autores principales: Tang, Yilong, Xu, Yan, Xiao, Zhifeng, Zhao, Yannan, Li, Jing, Han, Sufang, Chen, Lei, Dai, Bin, Wang, Ling, Chen, Bing, Wang, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428343/
https://www.ncbi.nlm.nih.gov/pubmed/28298644
http://dx.doi.org/10.1038/s41598-017-00087-x
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author Tang, Yilong
Xu, Yan
Xiao, Zhifeng
Zhao, Yannan
Li, Jing
Han, Sufang
Chen, Lei
Dai, Bin
Wang, Ling
Chen, Bing
Wang, Hong
author_facet Tang, Yilong
Xu, Yan
Xiao, Zhifeng
Zhao, Yannan
Li, Jing
Han, Sufang
Chen, Lei
Dai, Bin
Wang, Ling
Chen, Bing
Wang, Hong
author_sort Tang, Yilong
collection PubMed
description Bone marrow mesenchymal stem cells (BMSCs) are a good candidate for tissue engineering and clinical application. One of the challenges in its cell therapy is how to quickly obtain an adequate number of seed cells and meanwhile maintain suitable differentiation potential. In this study we combined three-dimensional (3D) collagen porous scaffolds with rotary cell culture system (RCCS) (RCCS-3D) to create a stereoscopic dynamic environment for the amplification of rat BMSCs in vitro. The results revealed that this RCCS-3D system could enhance BMSCs’ proliferation and colony formation, as well as maintain the differentiation potential compared with conventional static two-dimensional (2D) and 3D cell culture conditions. In addition, high-throughput microarray analysis showed that gene expressions of RCCS-3D system displayed significant differences in cell proliferation and differentiation compared with static-2D conditions. Thus, RCCS-3D system could provide an effective means for BMSCs cell proliferation in vitro and meanwhile maintain differentiation potential in tissue engineering.
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spelling pubmed-54283432017-05-15 The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs Tang, Yilong Xu, Yan Xiao, Zhifeng Zhao, Yannan Li, Jing Han, Sufang Chen, Lei Dai, Bin Wang, Ling Chen, Bing Wang, Hong Sci Rep Article Bone marrow mesenchymal stem cells (BMSCs) are a good candidate for tissue engineering and clinical application. One of the challenges in its cell therapy is how to quickly obtain an adequate number of seed cells and meanwhile maintain suitable differentiation potential. In this study we combined three-dimensional (3D) collagen porous scaffolds with rotary cell culture system (RCCS) (RCCS-3D) to create a stereoscopic dynamic environment for the amplification of rat BMSCs in vitro. The results revealed that this RCCS-3D system could enhance BMSCs’ proliferation and colony formation, as well as maintain the differentiation potential compared with conventional static two-dimensional (2D) and 3D cell culture conditions. In addition, high-throughput microarray analysis showed that gene expressions of RCCS-3D system displayed significant differences in cell proliferation and differentiation compared with static-2D conditions. Thus, RCCS-3D system could provide an effective means for BMSCs cell proliferation in vitro and meanwhile maintain differentiation potential in tissue engineering. Nature Publishing Group UK 2017-03-15 /pmc/articles/PMC5428343/ /pubmed/28298644 http://dx.doi.org/10.1038/s41598-017-00087-x Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Tang, Yilong
Xu, Yan
Xiao, Zhifeng
Zhao, Yannan
Li, Jing
Han, Sufang
Chen, Lei
Dai, Bin
Wang, Ling
Chen, Bing
Wang, Hong
The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs
title The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs
title_full The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs
title_fullStr The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs
title_full_unstemmed The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs
title_short The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs
title_sort combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat bmscs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428343/
https://www.ncbi.nlm.nih.gov/pubmed/28298644
http://dx.doi.org/10.1038/s41598-017-00087-x
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