Cargando…
Transcriptomic profile of tobacco in response to Phytophthora nicotianae infection
Black shank, caused by Phytophthora nicotianae (P. nicotianae), is a serious disease of cultivated tobacco (Nicotiana tabacum) worldwide. The interactions between tobacco and P. nicotianae are complex and the outcomes of the interactions depend on the tobacco genotype, P. nicotianae strain, and envi...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428407/ https://www.ncbi.nlm.nih.gov/pubmed/28341825 http://dx.doi.org/10.1038/s41598-017-00481-5 |
_version_ | 1783235812620500992 |
---|---|
author | Yang, Jian-Kang Tong, Zhi-Jun Fang, Dun-Huang Chen, Xue-Jun Zhang, Ke-Qin Xiao, Bing-Guang |
author_facet | Yang, Jian-Kang Tong, Zhi-Jun Fang, Dun-Huang Chen, Xue-Jun Zhang, Ke-Qin Xiao, Bing-Guang |
author_sort | Yang, Jian-Kang |
collection | PubMed |
description | Black shank, caused by Phytophthora nicotianae (P. nicotianae), is a serious disease of cultivated tobacco (Nicotiana tabacum) worldwide. The interactions between tobacco and P. nicotianae are complex and the outcomes of the interactions depend on the tobacco genotype, P. nicotianae strain, and environmental conditions. In this study, we used RNA-sequencing (RNA-Seq) to investigate and compare transcriptional changes in the stems of tobacco upon inoculation with P. nicotianae strain race 0. We used two tobacco varieties: RBST (named from resistance to black shank and tobacco mosaic virus), which was resistant to the P. nicotianae strain race 0, and Honghuadajinyuan (HD), which was susceptible to P. nicotianae race 0. Samples were collected 12 and 72-hour post inoculation (hpi). Analysis of differentially expressed genes (DEGs) and significantly enriched GO terms indicated that several basic defense mechanisms were suppressed in both varieties, which included response to wounding (GO: 0009611), and defense response to fungus (GO: 0050832). We also found some genes that may especially be related to mechanisms of resistance in RBST, such as the one encoding a chitinase. These results will provide a valuable resource for understanding the interactions between P. nicotianae and tobacco plants. |
format | Online Article Text |
id | pubmed-5428407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54284072017-05-15 Transcriptomic profile of tobacco in response to Phytophthora nicotianae infection Yang, Jian-Kang Tong, Zhi-Jun Fang, Dun-Huang Chen, Xue-Jun Zhang, Ke-Qin Xiao, Bing-Guang Sci Rep Article Black shank, caused by Phytophthora nicotianae (P. nicotianae), is a serious disease of cultivated tobacco (Nicotiana tabacum) worldwide. The interactions between tobacco and P. nicotianae are complex and the outcomes of the interactions depend on the tobacco genotype, P. nicotianae strain, and environmental conditions. In this study, we used RNA-sequencing (RNA-Seq) to investigate and compare transcriptional changes in the stems of tobacco upon inoculation with P. nicotianae strain race 0. We used two tobacco varieties: RBST (named from resistance to black shank and tobacco mosaic virus), which was resistant to the P. nicotianae strain race 0, and Honghuadajinyuan (HD), which was susceptible to P. nicotianae race 0. Samples were collected 12 and 72-hour post inoculation (hpi). Analysis of differentially expressed genes (DEGs) and significantly enriched GO terms indicated that several basic defense mechanisms were suppressed in both varieties, which included response to wounding (GO: 0009611), and defense response to fungus (GO: 0050832). We also found some genes that may especially be related to mechanisms of resistance in RBST, such as the one encoding a chitinase. These results will provide a valuable resource for understanding the interactions between P. nicotianae and tobacco plants. Nature Publishing Group UK 2017-03-24 /pmc/articles/PMC5428407/ /pubmed/28341825 http://dx.doi.org/10.1038/s41598-017-00481-5 Text en © The Author(s) 2017 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Yang, Jian-Kang Tong, Zhi-Jun Fang, Dun-Huang Chen, Xue-Jun Zhang, Ke-Qin Xiao, Bing-Guang Transcriptomic profile of tobacco in response to Phytophthora nicotianae infection |
title | Transcriptomic profile of tobacco in response to Phytophthora nicotianae infection |
title_full | Transcriptomic profile of tobacco in response to Phytophthora nicotianae infection |
title_fullStr | Transcriptomic profile of tobacco in response to Phytophthora nicotianae infection |
title_full_unstemmed | Transcriptomic profile of tobacco in response to Phytophthora nicotianae infection |
title_short | Transcriptomic profile of tobacco in response to Phytophthora nicotianae infection |
title_sort | transcriptomic profile of tobacco in response to phytophthora nicotianae infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428407/ https://www.ncbi.nlm.nih.gov/pubmed/28341825 http://dx.doi.org/10.1038/s41598-017-00481-5 |
work_keys_str_mv | AT yangjiankang transcriptomicprofileoftobaccoinresponsetophytophthoranicotianaeinfection AT tongzhijun transcriptomicprofileoftobaccoinresponsetophytophthoranicotianaeinfection AT fangdunhuang transcriptomicprofileoftobaccoinresponsetophytophthoranicotianaeinfection AT chenxuejun transcriptomicprofileoftobaccoinresponsetophytophthoranicotianaeinfection AT zhangkeqin transcriptomicprofileoftobaccoinresponsetophytophthoranicotianaeinfection AT xiaobingguang transcriptomicprofileoftobaccoinresponsetophytophthoranicotianaeinfection |