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Drosophila dyskerin is required for somatic stem cell homeostasis
Drosophila represents an excellent model to dissect the roles played by the evolutionary conserved family of eukaryotic dyskerins. These multifunctional proteins are involved in the formation of H/ACA snoRNP and telomerase complexes, both involved in essential cellular tasks. Since fly telomere inte...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428438/ https://www.ncbi.nlm.nih.gov/pubmed/28337032 http://dx.doi.org/10.1038/s41598-017-00446-8 |
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author | Vicidomini, Rosario Petrizzo, Arianna di Giovanni, Annamaria Cassese, Laura Lombardi, Antonella Anna Pragliola, Caterina Furia, Maria |
author_facet | Vicidomini, Rosario Petrizzo, Arianna di Giovanni, Annamaria Cassese, Laura Lombardi, Antonella Anna Pragliola, Caterina Furia, Maria |
author_sort | Vicidomini, Rosario |
collection | PubMed |
description | Drosophila represents an excellent model to dissect the roles played by the evolutionary conserved family of eukaryotic dyskerins. These multifunctional proteins are involved in the formation of H/ACA snoRNP and telomerase complexes, both involved in essential cellular tasks. Since fly telomere integrity is guaranteed by a different mechanism, we used this organism to investigate the specific role played by dyskerin in somatic stem cell maintenance. To this aim, we focussed on Drosophila midgut, a hierarchically organized and well characterized model for stemness analysis. Surprisingly, the ubiquitous loss of the protein uniquely affects the formation of the larval stem cell niches, without altering other midgut cell types. The number of adult midgut precursor stem cells is dramatically reduced, and this effect is not caused by premature differentiation and is cell-autonomous. Moreover, a few dispersed precursors found in the depleted midguts can maintain stem identity and the ability to divide asymmetrically, nor show cell-growth defects or undergo apoptosis. Instead, their loss is mainly specifically dependent on defective amplification. These studies establish a strict link between dyskerin and somatic stem cell maintenance in a telomerase-lacking organism, indicating that loss of stemness can be regarded as a conserved, telomerase-independent effect of dyskerin dysfunction. |
format | Online Article Text |
id | pubmed-5428438 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54284382017-05-15 Drosophila dyskerin is required for somatic stem cell homeostasis Vicidomini, Rosario Petrizzo, Arianna di Giovanni, Annamaria Cassese, Laura Lombardi, Antonella Anna Pragliola, Caterina Furia, Maria Sci Rep Article Drosophila represents an excellent model to dissect the roles played by the evolutionary conserved family of eukaryotic dyskerins. These multifunctional proteins are involved in the formation of H/ACA snoRNP and telomerase complexes, both involved in essential cellular tasks. Since fly telomere integrity is guaranteed by a different mechanism, we used this organism to investigate the specific role played by dyskerin in somatic stem cell maintenance. To this aim, we focussed on Drosophila midgut, a hierarchically organized and well characterized model for stemness analysis. Surprisingly, the ubiquitous loss of the protein uniquely affects the formation of the larval stem cell niches, without altering other midgut cell types. The number of adult midgut precursor stem cells is dramatically reduced, and this effect is not caused by premature differentiation and is cell-autonomous. Moreover, a few dispersed precursors found in the depleted midguts can maintain stem identity and the ability to divide asymmetrically, nor show cell-growth defects or undergo apoptosis. Instead, their loss is mainly specifically dependent on defective amplification. These studies establish a strict link between dyskerin and somatic stem cell maintenance in a telomerase-lacking organism, indicating that loss of stemness can be regarded as a conserved, telomerase-independent effect of dyskerin dysfunction. Nature Publishing Group UK 2017-03-23 /pmc/articles/PMC5428438/ /pubmed/28337032 http://dx.doi.org/10.1038/s41598-017-00446-8 Text en © The Author(s) 2017 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Vicidomini, Rosario Petrizzo, Arianna di Giovanni, Annamaria Cassese, Laura Lombardi, Antonella Anna Pragliola, Caterina Furia, Maria Drosophila dyskerin is required for somatic stem cell homeostasis |
title | Drosophila dyskerin is required for somatic stem cell homeostasis |
title_full | Drosophila dyskerin is required for somatic stem cell homeostasis |
title_fullStr | Drosophila dyskerin is required for somatic stem cell homeostasis |
title_full_unstemmed | Drosophila dyskerin is required for somatic stem cell homeostasis |
title_short | Drosophila dyskerin is required for somatic stem cell homeostasis |
title_sort | drosophila dyskerin is required for somatic stem cell homeostasis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428438/ https://www.ncbi.nlm.nih.gov/pubmed/28337032 http://dx.doi.org/10.1038/s41598-017-00446-8 |
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