Cargando…
SLC3A2 is upregulated in human osteosarcoma and promotes tumor growth through the PI3K/Akt signaling pathway
Growing evidence indicates that SLC3A2 (solute carrier family 3 member 2) is upregulated and correlates with tumor growth in multiple types of cancers, while the role of SLC3A2 in human osteosarcoma (OS) is rarely discussed. Thus, the aim of the present study was to demonstrate the expression of SLC...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428444/ https://www.ncbi.nlm.nih.gov/pubmed/28350098 http://dx.doi.org/10.3892/or.2017.5530 |
_version_ | 1783235821922418688 |
---|---|
author | Zhu, Bin Cheng, Dongdong Hou, Lei Zhou, Shumin Ying, Tao Yang, Qingcheng |
author_facet | Zhu, Bin Cheng, Dongdong Hou, Lei Zhou, Shumin Ying, Tao Yang, Qingcheng |
author_sort | Zhu, Bin |
collection | PubMed |
description | Growing evidence indicates that SLC3A2 (solute carrier family 3 member 2) is upregulated and correlates with tumor growth in multiple types of cancers, while the role of SLC3A2 in human osteosarcoma (OS) is rarely discussed. Thus, the aim of the present study was to demonstrate the expression of SLC3A2 in human osteosarcoma and reveal its biological function and the underlying mechanisms. RT-PCR, western blot analysis and immunohistochemistry (IHC) were used to assess the expression of SLC3A2 in OS samples and cell lines. Cell cycle, Cell Counting Kit-8 (CCK-8) and colony formation assays were used to test the cell survival capacity. To investigate the potential mechanism by which SLC3A2 regulates OS growth, we used a slide-based antibody array. We demonstrated that SLC3A2 was upregulated in OS cell lines as well as OS tissues. High expression of SLC3A2 was correlated with clinical stage and tumor size in OS. Reduced expression of SLC3A2 inhibited OS cell proliferation through G2/M phase arrest. Most importantly, we found that SLC3A2 may regulate OS growth through the PI3K/Akt signaling pathway. In conclusion, SLC3A2 is upregulated in OS and plays a crucial role in tumor growth. Targeting SLC3A2 may provide a new therapeutic strategy for OS. |
format | Online Article Text |
id | pubmed-5428444 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-54284442017-05-15 SLC3A2 is upregulated in human osteosarcoma and promotes tumor growth through the PI3K/Akt signaling pathway Zhu, Bin Cheng, Dongdong Hou, Lei Zhou, Shumin Ying, Tao Yang, Qingcheng Oncol Rep Articles Growing evidence indicates that SLC3A2 (solute carrier family 3 member 2) is upregulated and correlates with tumor growth in multiple types of cancers, while the role of SLC3A2 in human osteosarcoma (OS) is rarely discussed. Thus, the aim of the present study was to demonstrate the expression of SLC3A2 in human osteosarcoma and reveal its biological function and the underlying mechanisms. RT-PCR, western blot analysis and immunohistochemistry (IHC) were used to assess the expression of SLC3A2 in OS samples and cell lines. Cell cycle, Cell Counting Kit-8 (CCK-8) and colony formation assays were used to test the cell survival capacity. To investigate the potential mechanism by which SLC3A2 regulates OS growth, we used a slide-based antibody array. We demonstrated that SLC3A2 was upregulated in OS cell lines as well as OS tissues. High expression of SLC3A2 was correlated with clinical stage and tumor size in OS. Reduced expression of SLC3A2 inhibited OS cell proliferation through G2/M phase arrest. Most importantly, we found that SLC3A2 may regulate OS growth through the PI3K/Akt signaling pathway. In conclusion, SLC3A2 is upregulated in OS and plays a crucial role in tumor growth. Targeting SLC3A2 may provide a new therapeutic strategy for OS. D.A. Spandidos 2017-05 2017-03-27 /pmc/articles/PMC5428444/ /pubmed/28350098 http://dx.doi.org/10.3892/or.2017.5530 Text en Copyright: © Zhu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhu, Bin Cheng, Dongdong Hou, Lei Zhou, Shumin Ying, Tao Yang, Qingcheng SLC3A2 is upregulated in human osteosarcoma and promotes tumor growth through the PI3K/Akt signaling pathway |
title | SLC3A2 is upregulated in human osteosarcoma and promotes tumor growth through the PI3K/Akt signaling pathway |
title_full | SLC3A2 is upregulated in human osteosarcoma and promotes tumor growth through the PI3K/Akt signaling pathway |
title_fullStr | SLC3A2 is upregulated in human osteosarcoma and promotes tumor growth through the PI3K/Akt signaling pathway |
title_full_unstemmed | SLC3A2 is upregulated in human osteosarcoma and promotes tumor growth through the PI3K/Akt signaling pathway |
title_short | SLC3A2 is upregulated in human osteosarcoma and promotes tumor growth through the PI3K/Akt signaling pathway |
title_sort | slc3a2 is upregulated in human osteosarcoma and promotes tumor growth through the pi3k/akt signaling pathway |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428444/ https://www.ncbi.nlm.nih.gov/pubmed/28350098 http://dx.doi.org/10.3892/or.2017.5530 |
work_keys_str_mv | AT zhubin slc3a2isupregulatedinhumanosteosarcomaandpromotestumorgrowththroughthepi3kaktsignalingpathway AT chengdongdong slc3a2isupregulatedinhumanosteosarcomaandpromotestumorgrowththroughthepi3kaktsignalingpathway AT houlei slc3a2isupregulatedinhumanosteosarcomaandpromotestumorgrowththroughthepi3kaktsignalingpathway AT zhoushumin slc3a2isupregulatedinhumanosteosarcomaandpromotestumorgrowththroughthepi3kaktsignalingpathway AT yingtao slc3a2isupregulatedinhumanosteosarcomaandpromotestumorgrowththroughthepi3kaktsignalingpathway AT yangqingcheng slc3a2isupregulatedinhumanosteosarcomaandpromotestumorgrowththroughthepi3kaktsignalingpathway |